This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nishita, M.
Right arrow Articles by Mizuno, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nishita, M.
Right arrow Articles by Mizuno, K.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, February 2002, p. 774-783, Vol. 22, No. 3
0270-7306/01/$04.00+0     DOI: 10.1128/MCB.22.3.774-783.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Stromal Cell-Derived Factor 1{alpha} Activates LIM Kinase 1 and Induces Cofilin Phosphorylation for T-Cell Chemotaxis

Michiru Nishita,1 Hiroyuki Aizawa,2 and Kensaku Mizuno1*

Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Sendai 980-8578, Japan,1 Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 212052

Received 9 July 2001/ Returned for modification 6 August 2001/ Accepted 15 October 2001

Stromal cell-derived factor 1 {alpha} (SDF-1{alpha}), the ligand for G-protein-coupled receptor CXCR4, is a chemotactic factor for T lymphocytes. LIM kinase 1 (LIMK1) phosphorylates cofilin, an actin-depolymerizing and -severing protein, at Ser-3 and regulates actin reorganization. We investigated the role of cofilin phosphorylation by LIMK1 in SDF-1{alpha}-induced chemotaxis of T lymphocytes. SDF-1{alpha} significantly induced the activation of LIMK1 in Jurkat human leukemic T cells and peripheral blood lymphocytes. SDF-1{alpha} also induced cofilin phosphorylation, actin reorganization, and activation of small GTPases, Rho, Rac, and Cdc42, in Jurkat cells. Pretreatment with pertussis toxin inhibited SDF-1{alpha}-induced LIMK1 activation, thus indicating that Gi protein is involved in LIMK1 activation. Expression of dominant negative Rac (DN-Rac), but not DN-Rho or DN-Cdc42, blocked SDF-1{alpha}-induced activation of LIMK1, which means that SDF-1{alpha}-induced LIMK1 activation is mediated by Rac but not by Rho or Cdc42. We used a cell-permeable peptide (S3 peptide) that contains the phosphorylation site (Ser-3) of cofilin to inhibit the cellular function of LIMK1. S3 peptide inhibited the kinase activity of LIMK1 in vitro. Treatment of Jurkat cells with S3 peptide inhibited the SDF-1{alpha}-induced cofilin phosphorylation, actin reorganization, and chemotactic response of Jurkat cells. These results suggest that the phosphorylation of cofilin by LIMK1 plays a critical role in the SDF-1{alpha}-induced chemotactic response of T lymphocytes.

* Corresponding author. Mailing address: Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Aramaki-Aza-Aoba, Aoba-ku, Sendai 980-8578, Japan. Phone: 81-22-217-6676. Fax: 81-22-217-6678. E-mail: kmizuno{at}biology.tohoku.ac.jp.

Molecular and Cellular Biology, February 2002, p. 774-783, Vol. 22, No. 3
0022-538X/01/$04.00+0     DOI: 10.1128/MCB.22.3.774-783.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Vongsa, R. A., Zimmerman, N. P., Dwinell, M. B. (2009). CCR6 Regulation of the Actin Cytoskeleton Orchestrates Human Beta Defensin-2- and CCL20-mediated Restitution of Colonic Epithelial Cells. J. Biol. Chem. 284: 10034-10045 [Abstract] [Full Text]  
  • Horita, Y., Ohashi, K., Mukai, M., Inoue, M., Mizuno, K. (2008). Suppression of the Invasive Capacity of Rat Ascites Hepatoma Cells by Knockdown of Slingshot or LIM Kinase. J. Biol. Chem. 283: 6013-6021 [Abstract] [Full Text]  
  • Kaji, N., Muramoto, A., Mizuno, K. (2008). LIM Kinase-mediated Cofilin Phosphorylation during Mitosis Is Required for Precise Spindle Positioning. J. Biol. Chem. 283: 4983-4992 [Abstract] [Full Text]  
  • Wen, Z., Han, L., Bamburg, J. R., Shim, S., Ming, G.-l., Zheng, J. Q. (2007). BMP gradients steer nerve growth cones by a balancing act of LIM kinase and Slingshot phosphatase on ADF/cofilin. JCB 178: 107-119 [Abstract] [Full Text]  
  • Prasad, A., Qamri, Z., Wu, J., Ganju, R. K. (2007). Slit-2/Robo-1 modulates the CXCL12/CXCR4-induced chemotaxis of T cells. J. Leukoc. Biol. 82: 465-476 [Abstract] [Full Text]  
  • Endo, M., Ohashi, K., Mizuno, K. (2007). LIM Kinase and Slingshot Are Critical for Neurite Extension. J. Biol. Chem. 282: 13692-13702 [Abstract] [Full Text]  
  • Kurita, S., Gunji, E., Ohashi, K., Mizuno, K. (2007). Actin filaments-stabilizing and -bundling activities of cofilin-phosphatase Slingshot-1. GENES CELLS 12: 663-676 [Abstract] [Full Text]  
  • Hirayama, A., Adachi, R., Otani, S., Kasahara, T., Suzuki, K. (2007). Cofilin plays a critical role in IL-8-dependent chemotaxis of neutrophilic HL-60 cells through changes in phosphorylation. J. Leukoc. Biol. 81: 720-728 [Abstract] [Full Text]  
  • Tan, W., Martin, D., Gutkind, J. S. (2006). The G{alpha}13-Rho Signaling Axis Is Required for SDF-1-induced Migration through CXCR4. J. Biol. Chem. 281: 39542-39549 [Abstract] [Full Text]  
  • Song, X., Chen, X., Yamaguchi, H., Mouneimne, G., Condeelis, J. S., Eddy, R. J. (2006). Initiation of cofilin activity in response to EGF is uncoupled from cofilin phosphorylation and dephosphorylation in carcinoma cells. J. Cell Sci. 119: 2871-2881 [Abstract] [Full Text]  
  • Gallego, M. D., de la Fuente, M. A., Anton, I. M., Snapper, S., Fuhlbrigge, R., Geha, R. S. (2006). WIP and WASP play complementary roles in T cell homing and chemotaxis to SDF-1{alpha}. Int Immunol 18: 221-232 [Abstract] [Full Text]  
  • Nishita, M., Tomizawa, C., Yamamoto, M., Horita, Y., Ohashi, K., Mizuno, K. (2005). Spatial and temporal regulation of cofilin activity by LIM kinase and Slingshot is critical for directional cell migration. JCB 171: 349-359 [Abstract] [Full Text]  
  • Garcia-Bernal, D., Wright, N., Sotillo-Mallo, E., Nombela-Arrieta, C., Stein, J. V., Bustelo, X. R., Teixido, J. (2005). Vav1 and Rac Control Chemokine-promoted T Lymphocyte Adhesion Mediated by the Integrin {alpha}4{beta}1. Mol. Biol. Cell 16: 3223-3235 [Abstract] [Full Text]  
  • Wang, Y., Shibasaki, F., Mizuno, K. (2005). Calcium Signal-induced Cofilin Dephosphorylation Is Mediated by Slingshot via Calcineurin. J. Biol. Chem. 280: 12683-12689 [Abstract] [Full Text]  
  • Ghilardi, G., Biondi, M. L., La Torre, A., Battaglioli, L., Scorza, R. (2005). Breast Cancer Progression and Host Polymorphisms in the Chemokine System: Role of the Macrophage Chemoattractant Protein-1 (MCP-1) -2518 G Allele. Clin. Chem. 51: 452-455 [Full Text]  
  • Bonig, H., Priestley, G. V., Nilsson, L. M., Jiang, Y., Papayannopoulou, T. (2004). PTX-sensitive signals in bone marrow homing of fetal and adult hematopoietic progenitor cells. Blood 104: 2299-2306 [Abstract] [Full Text]  
  • Weiss-Haljiti, C., Pasquali, C., Ji, H., Gillieron, C., Chabert, C., Curchod, M.-L., Hirsch, E., Ridley, A. J., van Huijsduijnen, R. H., Camps, M., Rommel, C. (2004). Involvement of Phosphoinositide 3-Kinase {gamma}, Rac, and PAK Signaling in Chemokine-induced Macrophage Migration. J. Biol. Chem. 279: 43273-43284 [Abstract] [Full Text]  
  • Fischer, A. M., Mercer, J. C., Iyer, A., Ragin, M. J., August, A. (2004). Regulation of CXC Chemokine Receptor 4-mediated Migration by the Tec Family Tyrosine Kinase ITK. J. Biol. Chem. 279: 29816-29820 [Abstract] [Full Text]  
  • Pontow, S. E., Heyden, N. V., Wei, S., Ratner, L. (2004). Actin Cytoskeletal Reorganizations and Coreceptor-Mediated Activation of Rac during Human Immunodeficiency Virus-Induced Cell Fusion. J. Virol. 78: 7138-7147 [Abstract] [Full Text]  
  • Tomiyoshi, G., Horita, Y., Nishita, M., Ohashi, K., Mizuno, K. (2004). Caspase-mediated cleavage and activation of LIM-kinase 1 and its role in apoptotic membrane blebbing. GENES CELLS 9: 591-600 [Abstract] [Full Text]  
  • Wu, X., Li, D.-J., Yuan, M.-M., Zhu, Y., Wang, M.-Y. (2004). The Expression of CXCR4/CXCL12 in First-Trimester Human Trophoblast Cells. Biol. Reprod. 70: 1877-1885 [Abstract] [Full Text]  
  • Nagata-Ohashi, K., Ohta, Y., Goto, K., Chiba, S., Mori, R., Nishita, M., Ohashi, K., Kousaka, K., Iwamatsu, A., Niwa, R., Uemura, T., Mizuno, K. (2004). A pathway of neuregulin-induced activation of cofilin-phosphatase Slingshot and cofilin in lamellipodia. JCB 165: 465-471 [Abstract] [Full Text]  
  • Oonakahara, K.-i., Matsuyama, W., Higashimoto, I., Kawabata, M., Arimura, K., Osame, M. (2004). Stromal-Derived Factor-1{alpha}/CXCL12-CXCR 4 Axis Is Involved in the Dissemination of NSCLC Cells into Pleural Space. Am. J. Respir. Cell Mol. Bio. 30: 671-677 [Abstract] [Full Text]  
  • Ghosh, M., Song, X., Mouneimne, G., Sidani, M., Lawrence, D. S., Condeelis, J. S. (2004). Cofilin Promotes Actin Polymerization and Defines the Direction of Cell Motility. Science 304: 743-746 [Abstract] [Full Text]  
  • Sanchez-Martin, L., Sanchez-Sanchez, N., Gutierrez-Lopez, M. D., Rojo, A. I., Vicente-Manzanares, M., Perez-Alvarez, M. J., Sanchez-Mateos, P., Bustelo, X. R., Cuadrado, A., Sanchez-Madrid, F., Rodriguez-Fernandez, J. L., Cabanas, C. (2004). Signaling through the Leukocyte Integrin LFA-1 in T Cells Induces a Transient Activation of Rac-1 That Is Regulated by Vav and PI3K/Akt-1. J. Biol. Chem. 279: 16194-16205 [Abstract] [Full Text]  
  • Konakahara, S., Ohashi, K., Mizuno, K., Itoh, K., Tsuji, T. (2004). CD29 integrin- and LIMK1/cofilin-mediated actin reorganization regulates the migration of haematopoietic progenitor cells underneath bone marrow stromal cells. GENES CELLS 9: 345-358 [Abstract] [Full Text]  
  • Nishita, M., Wang, Y., Tomizawa, C., Suzuki, A., Niwa, R., Uemura, T., Mizuno, K. (2004). Phosphoinositide 3-Kinase-mediated Activation of Cofilin Phosphatase Slingshot and Its Role for Insulin-induced Membrane Protrusion. J. Biol. Chem. 279: 7193-7198 [Abstract] [Full Text]  
  • Yoshioka, K., Foletta, V., Bernard, O., Itoh, K. (2003). A role for LIM kinase in cancer invasion. Proc. Natl. Acad. Sci. USA 100: 7247-7252 [Abstract] [Full Text]  
  • Lui, W.-y., Lee, W. M., Cheng, C. Y. (2003). Sertoli-Germ Cell Adherens Junction Dynamics in the Testis Are Regulated by RhoB GTPase via the ROCK/LIMK Signaling Pathway. Biol. Reprod. 68: 2189-2206 [Abstract] [Full Text]  
  • Hall, J. M., Korach, K. S. (2003). Stromal Cell-Derived Factor 1, a Novel Target of Estrogen Receptor Action, Mediates the Mitogenic Effects of Estradiol in Ovarian and Breast Cancer Cells. Mol. Endocrinol. 17: 792-803 [Abstract] [Full Text]  
  • Samstag, Y., Eibert, S. M., Klemke, M., Wabnitz, G. H. (2003). Actin cytoskeletal dynamics in T lymphocyte activation and migration. J. Leukoc. Biol. 73: 30-48 [Abstract] [Full Text]  
  • Adachi, R., Takeuchi, K., Suzuki, K. (2002). Antisense Oligonucleotide to Cofilin Enhances Respiratory Burst and Phagocytosis in Opsonized Zymosan-stimulated Mouse Macrophage J774.1 Cells. J. Biol. Chem. 277: 45566-45571 [Abstract] [Full Text]  
  • Amano, T., Kaji, N., Ohashi, K., Mizuno, K. (2002). Mitosis-specific Activation of LIM Motif-containing Protein Kinase and Roles of Cofilin Phosphorylation and Dephosphorylation in Mitosis. J. Biol. Chem. 277: 22093-22102 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»