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Molecular and Cellular Biology, February 2002, p. 856-865, Vol. 22, No. 3
0270-7306/01/$04.00+0     DOI: 10.1128/MCB.22.3.856-865.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Retinoblastoma Protein Transcriptional Repression through Histone Deacetylation of a Single Nucleosome

Ashby J. Morrison,1 Claude Sardet,2 and Rafael E. Herrera1*

Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030,1 Institut de Génétique Moléculaire, UMR 5535, CNRS, 34293 Montpellier Cedex 5, France2

Received 10 September 2001/ Returned for modification 11 October 2001/ Accepted 16 October 2001

The retinoblastoma protein, pRb, controls transcription through recruitment of histone deacetylase to particular E2F-responsive genes. We determined the acetylation level of individual nucleosomes present in the cyclin E promoter of RB+/+ and RB-/- mouse embryo fibroblasts. We also determined the effects of pRb on nucleosomal conformation by examining the thiol reactivity of histone H3 of individual nucleosomes. We found that pRb represses the cyclin E promoter through histone deacetylation of a single nucleosome, to which it and histone deacetylase 1 bind. In addition, the conformation of this nucleosome is modulated by pRb-directed histone deacetylase activity. Thus, the repressive role of pRb in cyclin E transcription and therefore cell cycle progression can be mapped to its control of the acetylation status and conformation of a single nucleosome.

* Corresponding author. Mailing address: Baylor College of Medicine, Department of Molecular and Cellular Biology, The Breast Center, One Baylor Plaza, Houston, TX 77030. Phone: (713) 798-1600. Fax: (713) 798-1642. E-mail: rherrera{at}bcm.tmc.edu.

Molecular and Cellular Biology, February 2002, p. 856-865, Vol. 22, No. 3
0022-538X/01/$04.00+0     DOI: 10.1128/MCB.22.3.856-865.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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