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Molecular and Cellular Biology, March 2002, p. 1778-1791, Vol. 22, No. 6
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.6.1778-1791.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

A Novel and Conserved Protein-Protein Interaction Domain of Mammalian Lin-2/CASK Binds and Recruits SAP97 to the Lateral Surface of Epithelia

Seonok Lee,1 Shuling Fan,2 Olya Makarova,2 Samuel Straight,2 and Ben Margolis1,2,3*

Howard Hughes Medical InstituteDepartments of,2 Biological Chemistry,1 Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 481093

Received 17 July 2001/ Returned for modification 18 September 2001/ Accepted 5 December 2001

Mammalian Lin-2 (mLin-2)/CASK is a membrane-associated guanylate kinase (MAGUK) and contains multidomain modules that mediate protein-protein interactions important for the establishment and maintenance of neuronal and epithelial cell polarization. The importance of mLin-2/CASK in mammalian development is demonstrated by the fact that mutations in mLin-2/CASK or SAP97, another MAGUK protein, lead to cleft palate in mice. We recently identified a new protein-protein interaction domain, called the L27 domain, which is present twice in mLin-2/CASK. In this report, we further define the binding of the L27C domain of mLin-2/CASK to the L27 domain of mLin-7 and identify the binding partner for L27N of mLin-2/CASK. Biochemical analysis reveals that this L27N domain binds to the N terminus of SAP97, a region that was previously reported to be essential for the lateral membrane recruitment of SAP97 in epithelia. Our colocalization studies, using dominant-negative mLin-2/CASK, show that the association with mLin-2/CASK is crucial for lateral localization of SAP97 in MDCK cells. We also report the identification of a novel isoform of Discs Large, a Drosophila melanogaster orthologue of SAP97, which contains a region highly related to the SAP97 N terminus and which binds Camguk, a Drosophila orthologue of mLin-2/CASK. Our data identify evolutionarily conserved protein-protein interaction domains that link mLin-2/CASK to SAP97 and account for their common phenotype when mutated in mice.

* Corresponding author. Mailing address: Howard Hughes Medical Institute, University of Michigan Medical Center, 4570 MSRB II, Box 0650, 1150 W. Medical Center Dr., Ann Arbor, MI 48109-0650. Phone: (734) 764-3567. Fax: (734) 763-9323. E-mail: bmargoli{at}umich.edu.

Molecular and Cellular Biology, March 2002, p. 1778-1791, Vol. 22, No. 6
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.6.1778-1791.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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