MCB
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hauck, L.
Right arrow Articles by von Harsdorf, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hauck, L.
Right arrow Articles by von Harsdorf, R.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, April 2002, p. 2147-2158, Vol. 22, No. 7
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.7.2147-2158.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Regulation of E2F1-Dependent Gene Transcription and Apoptosis by the ETS-Related Transcription Factor GABP{gamma}1

Ludger Hauck,1,2 Rudolf G. Kaba,2 Martin Lipp,2 Rainer Dietz,1,2 and Rüdiger von Harsdorf1,2*

Department of Cardiology, Campus Virchow Clinic, Charité, Humboldt University,1 Max Delbrück Center for Molecular Medicine, Berlin, Germany2

Received 26 November 2001/ Accepted 14 December 2001

The E2F family of transcription factors comprises six related members which are involved in the control of the coordinated progression through the G1/S-phase transition of cell cycle or in cell fate decision. Their activity is regulated by pocket proteins, including pRb, p107, and p130. Here we show that E2F1 directly interacts with the ETS-related transcription factor GABP{gamma}1 in vitro and in vivo. The binding domain interacting with GABP{gamma}1 was mapped to the C-terminal amino acids 310 to 437 of E2F1, which include its transactivation and pRb binding domain. Among the E2F family of transcription factors, the interaction with GABP{gamma}1 is restricted to E2F1. DNA-binding E2F1 complexes containing GABP{gamma}1 are characterized by enhanced E2F1-dependent transcriptional activity. Moreover, GABP{gamma}1 suppresses E2F1-dependent apoptosis by mechanisms other than the inhibition of the transactivation capacity of E2F1. In summary, our results provide evidence for a novel pRb-independent mechanism regulating E2F1-dependent transcription and apoptosis.

* Corresponding author. Mailing address: Medizinische Klinik mit Schwerpunkt Kardiologie, Universitätsklinikum Charité, Humboldt-Universität zu Berlin, Augustenburger Platz 1, 13353 Berlin, Germany. Phone: 49-30-450 5537 47. Fax: 49-30-450 5539 77. E-mail: rharsdo{at}mdc-berlin.de.

Molecular and Cellular Biology, April 2002, p. 2147-2158, Vol. 22, No. 7
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.7.2147-2158.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. J. Virol. Eukaryot. Cell
Microbiol. Mol. Biol. Rev. Clin. Vaccine Immunol. All ASM Journals

Copyright © 2002 by the American Society for Microbiology. All rights reserved.