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Molecular and Cellular Biology, April 2002, p. 2598-2606, Vol. 22, No. 8
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.8.2598-2606.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Scaffold/Matrix Attachment Region Elements Interact with a p300-Scaffold Attachment Factor A Complex and Are Bound by Acetylated Nucleosomes

Joost H. A. Martens, Matty Verlaan, Eric Kalkhoven, Josephine C. Dorsman, and Alt Zantema*

Molecular Cell Biology, Centre for Biomedical Genetics, Leiden University Medical Center, Leiden, The Netherlands

Received 26 October 2001/ Returned for modification 17 December 2001/ Accepted 30 January 2002

The transcriptional coactivator p300 regulates transcription by binding to proteins involved in transcription and by acetylating histones and other proteins. These transcriptional effects are mainly at promoter and enhancer elements. Regulation of transcription also occurs through scaffold/matrix attachment regions (S/MARs), the chromatin regions that bind the nuclear matrix. Here we show that p300 binds to the S/MAR binding protein scaffold attachment factor A (SAF-A), a major constituent of the nuclear matrix. Using chromatin immunoprecipitations, we established that both p300 and SAF-A bind to S/MAR elements in the transiently silent topoisomerase I gene prior to its activation at G1 during cell cycle. This binding is accompanied by local acetylation of nucleosomes, suggesting that p300-SAF-A interactions at S/MAR elements of nontranscribed genes might poise these genes for transcription.

* Corresponding author. Mailing address: MCB, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands. Phone: 31(0)71-5276113. Fax: 31(0)71-5276284. E-mail:zantema{at}lumc.nl.

Molecular and Cellular Biology, April 2002, p. 2598-2606, Vol. 22, No. 8
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.8.2598-2606.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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