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Molecular and Cellular Biology, April 2002, p. 2761-2768, Vol. 22, No. 8
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.8.2761-2768.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Reduced Sperm Count and Normal Fertility in Male Mice with Targeted Disruption of the ADP-Ribosylation Factor-Like 4 (Arl4) Gene

A. Schürmann,1* S. Koling,1 S. Jacobs,1 P. Saftig,2 S. Krauß,1 G. Wennemuth,3 R. Kluge,4 and H.-G. Joost1

Institute of Pharmacology,1 Institute of Animal Research, Medical Faculty, Technical University of Aachen, D-52057 Aachen,4 Department of Biochemistry, University of Kiel, D-24118 Kiel,2 Department of Anatomy and Cell Biology, University of Marburg, D-35037 Marburg, Germany3

Received 14 November 2001/ Returned for modification 18 December 2001/ Accepted 15 January 2002

The ADP-ribosylation factor-like protein 4 (ARL4) is a 22-kDa GTP-binding protein which is abundant in testes of pubertal and adult rodents but absent in testes from prepubertal animals. During testis development, ARL4 expression starts at day 16 when the spermatogenesis proceeds to the late pachytene. In the adult testis, the ARL4 protein was detected in pre- and postmeiotic cells, spermatocytes, and spermatides, but not in spermatogonia and mature spermatozoa. Mouse Arl4-null mutants generated by targeted disruption of the Arl4 gene were viable and grew normally; male as well as female Arl4-/- mice were fertile. However, inactivation of the Arl4 gene resulted in a significant reduction of testis weight and sperm count by 30 and 60%, respectively, without reduction of litter size or frequency. It is suggested that the disruption of Arl4 produces a moderate retardation of germ cell development, possibly at the stage of meiosis.

* Corresponding author. Mailing address: Institute of Pharmacology, Medical Faculty, Technical University of Aachen, D-52057 Aachen, Germany. Phone: 49-241-8089137. Fax: 49-241-8082433. E-mail: aschuermann{at}ukaachen.de.

Molecular and Cellular Biology, April 2002, p. 2761-2768, Vol. 22, No. 8
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.8.2761-2768.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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