This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wiggin, G. R. Articles by Arthur, J. S. C. Search for Related Content PubMed PubMed Citation Articles by Wiggin, G. R. Articles by Arthur, J. S. C.

 Previous Article

Molecular and Cellular Biology, April 2002, p. 2871-2881, Vol. 22, No. 8
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.8.2871-2881.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

MSK1 and MSK2 Are Required for the Mitogen- and Stress-Induced Phosphorylation of CREB and ATF1 in Fibroblasts

MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland

Received 17 October 2001/ Returned for modification 20 November 2001/ Accepted 7 January 2002

Using mouse knockouts for mitogen- and stress-activated protein kinase 1 (MSK1) and MSK2 and a double knockout of both MSK1 and MSK2, we show that these protein kinases are required for the stress-induced phosphorylation of transcription factors CREB and ATF1 in primary embryonic fibroblasts. In contrast mitogen-induced phosphorylation of CREB and ATF1 is greatly reduced but not totally abolished. The mitogen- and stress-induced phosphorylation of CREB at Ser133 has been linked to the transcription of several immediate early genes, including c-fos, junB, and egr1. The knockout of both MSK1 and MSK2 resulted in a 50% reduction in c-fos and junB gene transcription in response to anisomycin or UV-C radiation but only a small reduction in response to tetradecanoyl phorbol acetate or epidermal growth factor in fibroblasts. The transcription of egr1 in response to both mitogenic and stress stimuli, as well as stress-induced apoptosis, was unaffected in the MSK1/MSK2 double knockout.

This article has been cited by other articles:

• Ozgen, N., Obreztchikova, M., Guo, J., Elouardighi, H., Dorn, G. W. II, Wilson, B. A., Steinberg, S. F. (2008). Protein Kinase D Links Gq-coupled Receptors to cAMP Response Element-binding Protein (CREB)-Ser133 Phosphorylation in the Heart. J. Biol. Chem. 283: 17009-17019 [Abstract] [Full Text]  
• Kim, H.-G., Lee, K. W., Cho, Y.-Y., Kang, N. J., Oh, S.-M., Bode, A. M., Dong, Z. (2008). Mitogen- and Stress-Activated Kinase 1-Mediated Histone H3 Phosphorylation Is Crucial for Cell Transformation. Cancer Res. 68: 2538-2547 [Abstract] [Full Text]  
• Goplen, N., Gorska, M. M., Stafford, S. J., Rozario, S., Guo, L., Liang, Q., Alam, R. (2008). A Phosphosite Screen Identifies Autocrine TGF-{beta}-Driven Activation of Protein Kinase R as a Survival-Limiting Factor for Eosinophils. J. Immunol. 180: 4256-4264 [Abstract] [Full Text]  
• Nwachukwu, J. C., Li, W., Pineda-Torra, I., Huang, H. Y., Ruoff, R., Shapiro, E., Taneja, S. S., Logan, S. K., Garabedian, M. J. (2007). Transcriptional Regulation of the Androgen Receptor Cofactor Androgen Receptor Trapped Clone-27. Mol. Endocrinol. 21: 2864-2876 [Abstract] [Full Text]  
• Chwang, W. B., Arthur, J. S., Schumacher, A., Sweatt, J. D. (2007). The Nuclear Kinase Mitogen- and Stress-Activated Protein Kinase 1 Regulates Hippocampal Chromatin Remodeling in Memory Formation. J. Neurosci. 27: 12732-12742 [Abstract] [Full Text]  
• Schindler, J.F., Monahan, J.B., Smith, W.G. (2007). p38 Pathway Kinases as Anti-inflammatory Drug Targets. J. Dent. Res. 86: 800-811 [Abstract] [Full Text]  
• Keller, M. J., Wu, A. W., Andrews, J. I., McGonagill, P. W., Tibesar, E. E., Meier, J. L. (2007). Reversal of Human Cytomegalovirus Major Immediate-Early Enhancer/Promoter Silencing in Quiescently Infected Cells via the Cyclic AMP Signaling Pathway. J. Virol. 81: 6669-6681 [Abstract] [Full Text]  
• Shanware, N. P., Trinh, A. T., Williams, L. M., Tibbetts, R. S. (2007). Coregulated Ataxia Telangiectasia-mutated and Casein Kinase Sites Modulate cAMP-response Element-binding Protein-Coactivator Interactions in Response to DNA Damage. J. Biol. Chem. 282: 6283-6291 [Abstract] [Full Text]  
• Shi, G.-X., Rehmann, H., Andres, D. A. (2006). A Novel Cyclic AMP-Dependent Epac-Rit Signaling Pathway Contributes to PACAP38-Mediated Neuronal Differentiation. Mol. Cell. Biol. 26: 9136-9147 [Abstract] [Full Text]  
• Kannan-Thulasiraman, P., Katsoulidis, E., Tallman, M. S., Arthur, J. S. C., Platanias, L. C. (2006). Activation of the Mitogen- and Stress-activated Kinase 1 by Arsenic Trioxide. J. Biol. Chem. 281: 22446-22452 [Abstract] [Full Text]  
• Hauge, C., Frodin, M. (2006). RSK and MSK in MAP kinase signalling.. J. Cell Sci. 119: 3021-3023 [Full Text]  
• Das, S., Tosaki, A., Bagchi, D., Maulik, N., Das, D. K. (2006). Potentiation of a Survival Signal in the Ischemic Heart by Resveratrol through p38 Mitogen-Activated Protein Kinase/Mitogen- and Stress-Activated Protein Kinase 1/cAMP Response Element-Binding Protein Signaling. J. Pharmacol. Exp. Ther. 317: 980-988 [Abstract] [Full Text]  
• Vanden Berghe, W., Dijsselbloem, N., Vermeulen, L., Ndlovu, M. N., Boone, E., Haegeman, G. (2006). Attenuation of Mitogen- and Stress-Activated Protein Kinase-1-Driven Nuclear Factor-{kappa}B Gene Expression by Soy Isoflavones Does Not Require Estrogenic Activity.. Cancer Res. 66: 4852-4862 [Abstract] [Full Text]  
• Brami-Cherrier, K., Valjent, E., Herve, D., Darragh, J., Corvol, J.-C., Pages, C., Simon, A. J., Girault, J.-A., Caboche, J. (2005). Parsing Molecular and Behavioral Effects of Cocaine in Mitogen- and Stress-Activated Protein Kinase-1-Deficient Mice. J. Neurosci. 25: 11444-11454 [Abstract] [Full Text]  
• Beardmore, V. A., Hinton, H. J., Eftychi, C., Apostolaki, M., Armaka, M., Darragh, J., McIlrath, J., Carr, J. M., Armit, L. J., Clacher, C., Malone, L., Kollias, G., Arthur, J. S. C. (2005). Generation and Characterization of p38{beta} (MAPK11) Gene-Targeted Mice. Mol. Cell. Biol. 25: 10454-10464 [Abstract] [Full Text]  
• Hokari, R., Lee, H., Crawley, S. C., Yang, S. C., Gum, J. R. Jr, Miura, S., Kim, Y. S. (2005). Vasoactive intestinal peptide upregulates MUC2 intestinal mucin via CREB/ATF1. Am. J. Physiol. Gastrointest. Liver Physiol. 289: G949-G959 [Abstract] [Full Text]  
• Wang, J.-M., Tseng, J. T., Chang, W.-C. (2005). Induction of Human NF-IL6{beta} by Epidermal Growth Factor Is Mediated through the p38 Signaling Pathway and cAMP Response Element-binding Protein Activation in A431 Cells. Mol. Biol. Cell 16: 3365-3376 [Abstract] [Full Text]  
• Butcher, G. Q., Lee, B., Cheng, H.-Y. M., Obrietan, K. (2005). Light Stimulates MSK1 Activation in the Suprachiasmatic Nucleus via a PACAP-ERK/MAP Kinase-Dependent Mechanism. J. Neurosci. 25: 5305-5313 [Abstract] [Full Text]  
• Dyson, M. H., Thomson, S., Inagaki, M., Goto, H., Arthur, S. J., Nightingale, K., Iborra, F. J., Mahadevan, L. C. (2005). MAP kinase-mediated phosphorylation of distinct pools of histone H3 at S10 or S28 via mitogen- and stress-activated kinase 1/2. J. Cell Sci. 118: 2247-2259 [Abstract] [Full Text]  
• Bode, A. M., Dong, Z. (2005). Inducible Covalent Posttranslational Modification of Histone H3. Sci Signal 2005: re4-re4 [Abstract] [Full Text]  
• Rakhit, S., Clark, C. J., O'Shaughnessy, C. T., Morris, B. J. (2005). N-Methyl-D-aspartate and Brain-Derived Neurotrophic Factor Induce Distinct Profiles of Extracellular Signal-Regulated Kinase, Mitogen- and Stress-Activated Kinase, and Ribosomal S6 Kinase Phosphorylation in Cortical Neurons. Mol. Pharmacol. 67: 1158-1165 [Abstract] [Full Text]  
• Togo, T. (2004). Long-term Potentiation of Wound-induced Exocytosis and Plasma Membrane Repair Is Dependant on cAMP-response Element-mediated Transcription via a Protein Kinase C- and p38 MAPK-dependent Pathway. J. Biol. Chem. 279: 44996-45003 [Abstract] [Full Text]  
• Roux, P. P., Blenis, J. (2004). ERK and p38 MAPK-Activated Protein Kinases: a Family of Protein Kinases with Diverse Biological Functions. Microbiol. Mol. Biol. Rev. 68: 320-344 [Abstract] [Full Text]  
• Simon, J., Arthur, C., Fong, A. L., Dwyer, J. M., Davare, M., Reese, E., Obrietan, K., Impey, S. (2004). Mitogen- and Stress-Activated Protein Kinase 1 Mediates cAMP Response Element-Binding Protein Phosphorylation and Activation by Neurotrophins. J. Neurosci. 24: 4324-4332 [Abstract] [Full Text]  
• Sawka-Verhelle, D., Escoubet-Lozach, L., Fong, A. L., Hester, K. D., Herzig, S., Lebrun, P., Glass, C. K. (2004). PE-1/METS, an Antiproliferative Ets Repressor Factor, Is Induced by CREB-1/CREM-1 during Macrophage Differentiation. J. Biol. Chem. 279: 17772-17784 [Abstract] [Full Text]  
• Smith, J. L., Collins, I., Chandramouli, G. V. R., Butscher, W. G., Zaitseva, E., Freebern, W. J., Haggerty, C. M., Doseeva, V., Gardner, K. (2003). Targeting Combinatorial Transcriptional Complex Assembly at Specific Modules within the Interleukin-2 Promoter by the Immunosuppressant SB203580. J. Biol. Chem. 278: 41034-41046 [Abstract] [Full Text]  
• Song, K. S., Seong, J.-K., Chung, K. C., Lee, W.-J., Kim, C.-H., Cho, K. N., Kang, C.-D., Koo, J. S., Yoon, J.-H. (2003). Induction of MUC8 Gene Expression by Interleukin-1{beta} Is Mediated by a Sequential ERK MAPK/RSK1/CREB Cascade Pathway in Human Airway Epithelial Cells. J. Biol. Chem. 278: 34890-34896 [Abstract] [Full Text]  
• Mora-Garcia, P., Cheng, J., Crans-Vargas, H. N., Countouriotis, A., Shankar, D., Sakamoto, K. M. (2003). Transcriptional Regulators and Myelopoiesis: The Role of Serum Response Factor and CREB as Targets of Cytokine Signaling. Stem Cells 21: 123-130 [Abstract] [Full Text]  
• Gupta, P., Prywes, R. (2002). ATF1 Phosphorylation by the ERK MAPK Pathway Is Required for Epidermal Growth Factor-induced c-jun Expression. J. Biol. Chem. 277: 50550-50556 [Abstract] [Full Text]  
• Hamm, J., Alessi, D. R., Biondi, R. M. (2002). Bi-functional, Substrate Mimicking RNA Inhibits MSK1-mediated cAMP-response Element-binding Protein Phosphorylation and Reveals Magnesium Ion-dependent Conformational Changes of the Kinase. J. Biol. Chem. 277: 45793-45802 [Abstract] [Full Text]