This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Asahara, H. Articles by Montminy, M. Search for Related Content PubMed PubMed Citation Articles by Asahara, H. Articles by Montminy, M.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, May 2002, p. 2974-2983, Vol. 22, No. 9
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.9.2974-2983.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Dual Roles of p300 in Chromatin Assembly and Transcriptional Activation in Cooperation with Nucleosome Assembly Protein 1 In Vitro

Hiroshi Asahara,^1,2 Sophie Tartare-Deckert,^1,3, Takeya Nakagawa,⁴ Tsuyoshi Ikehara,⁴ Fumiko Hirose,⁵ Tony Hunter,³ Takashi Ito,^4* and Marc Montminy^1*

Peptide Biology Laboratory,¹ Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037,³ Division of Gene Structure and Function, Saitama Medical School Research Center for Genomic Medicine, Morohongo Moroyama, Iruma, Saitama 350-0495,⁴ Laboratory of Cell Biology, Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya 464-8681,⁵ PRESTO, Japan Science and Technology Corporation, Kawaguchi 332-0012, Japan²

Received 2 July 2001/ Returned for modification 26 October 2001/ Accepted 29 January 2002

In a yeast two-hybrid screen to identify proteins that bind to the KIX domain of the coactivator p300, we obtained cDNAs encoding nucleosome assembly protein 1 (NAP-1), a 60-kDa histone H2A-H2B shuttling protein that promotes histone deposition. p300 associates preferentially with the H2A-H2B-bound form of NAP-1 rather than with the unbound form of NAP-1. Formation of NAP-1-p300 complexes was found to increase during S phase, suggesting a potential role for p300 in chromatin assembly. In micrococcal nuclease and supercoiling assays, addition of p300 promoted efficient chromatin assembly in vitro in conjunction with NAP-1 and ATP-utilizing chromatin assembly and remodeling factor; this effect was dependent in part on the intrinsic histone acetyltransferase activity of p300. Surprisingly, NAP-1 potently inhibited acetylation of core histones by p300, suggesting that efficient assembly requires acetylation of either NAP-1 or p300 itself. As p300 acted cooperatively with NAP-1 in stimulating transcription from a chromatin template in vitro, our results suggest a dual role of NAP-1-p300 complexes in promoting chromatin assembly and transcriptional activation.

* Corresponding author. Mailing address for Marc Montminy: Salk Institute, 10010 N. Torrey Pines Rd., La Jolla, CA 92037. Phone: (858) 453-4100, ext. 1394. Fax: (858) 625-9045. E-mail: montminy{at}salk.edu.

Present address: INSERM Unité 145, IFR 50, Faculté de Médecine, Nice 06107, France.

This article has been cited by other articles:

• Sharma, N., Nyborg, J. K. (2008). The coactivators CBP/p300 and the histone chaperone NAP1 promote transcription-independent nucleosome eviction at the HTLV-1 promoter. Proc. Natl. Acad. Sci. USA 105: 7959-7963 [Abstract] [Full Text]  
• Tachiwana, H., Osakabe, A., Kimura, H., Kurumizaka, H. (2008). Nucleosome formation with the testis-specific histone H3 variant, H3t, by human nucleosome assembly proteins in vitro. Nucleic Acids Res 36: 2208-2218 [Abstract] [Full Text]  
• Clark, J., Alvarez, D. F., Alexeyev, M., King, J. A. C., Huang, L., Yoder, M. C., Stevens, T. (2008). Regulatory role for nucleosome assembly protein-1 in the proliferative and vasculogenic phenotype of pulmonary endothelium. Am. J. Physiol. Lung Cell. Mol. Physiol. 294: L431-L439 [Abstract] [Full Text]  
• Attia, M., Rachez, C., De Pauw, A., Avner, P., Rogner, U. C. (2007). Nap1l2 Promotes Histone Acetylation Activity during Neuronal Differentiation. Mol. Cell. Biol. 27: 6093-6102 [Abstract] [Full Text]  
• Eckey, M., Hong, W., Papaioannou, M., Baniahmad, A. (2007). The Nucleosome Assembly Activity of NAP1 Is Enhanced by Alien. Mol. Cell. Biol. 27: 3557-3568 [Abstract] [Full Text]  
• Zlatanova, J., Seebart, C., Tomschik, M. (2007). Nap1: taking a closer look at a juggler protein of extraordinary skills. FASEB J. 21: 1294-1310 [Abstract] [Full Text]  
• Galichet, A., Gruissem, W. (2006). Developmentally Controlled Farnesylation Modulates AtNAP1;1 Function in Cell Proliferation and Cell Expansion during Arabidopsis Leaf Development. Plant Physiol. 142: 1412-1426 [Abstract] [Full Text]  
• Kasper, L. H., Fukuyama, T., Biesen, M. A., Boussouar, F., Tong, C., de Pauw, A., Murray, P. J., van Deursen, J. M. A., Brindle, P. K. (2006). Conditional Knockout Mice Reveal Distinct Functions for the Global Transcriptional Coactivators CBP and p300 in T-Cell Development. Mol. Cell. Biol. 26: 789-809 [Abstract] [Full Text]  
• Furumatsu, T., Tsuda, M., Yoshida, K., Taniguchi, N., Ito, T., Hashimoto, M., Ito, T., Asahara, H. (2005). Sox9 and p300 Cooperatively Regulate Chromatin-mediated Transcription. J. Biol. Chem. 280: 35203-35208 [Abstract] [Full Text]  
• Abu-Daya, A., Steer, W. M., Trollope, A. F., Friedeberg, C. E., Patient, R. K., Thorne, A. W., Guille, M. J. (2005). Zygotic nucleosome assembly protein-like 1 has a specific, non-cell autonomous role in hematopoiesis. Blood 106: 514-520 [Abstract] [Full Text]  
• Dong, A., Liu, Z., Zhu, Y., Yu, F., Li, Z., Cao, K., Shen, W.-H. (2005). Interacting Proteins and Differences in Nuclear Transport Reveal Specific Functions for the NAP1 Family Proteins in Plants. Plant Physiol. 138: 1446-1456 [Abstract] [Full Text]  
• Toth, K. F., Mazurkiewicz, J., Rippe, K. (2005). Association States of Nucleosome Assembly Protein 1 and Its Complexes with Histones. J. Biol. Chem. 280: 15690-15699 [Abstract] [Full Text]  
• Furumatsu, T., Tsuda, M., Taniguchi, N., Tajima, Y., Asahara, H. (2005). Smad3 Induces Chondrogenesis through the Activation of SOX9 via CREB-binding Protein/p300 Recruitment. J. Biol. Chem. 280: 8343-8350 [Abstract] [Full Text]  
• Park, Y.-J., Chodaparambil, J. V., Bao, Y., McBryant, S. J., Luger, K. (2005). Nucleosome Assembly Protein 1 Exchanges Histone H2A-H2B Dimers and Assists Nucleosome Sliding. J. Biol. Chem. 280: 1817-1825 [Abstract] [Full Text]  
• Rehtanz, M., Schmidt, H.-M., Warthorst, U., Steger, G. (2004). Direct Interaction between Nucleosome Assembly Protein 1 and the Papillomavirus E2 Proteins Involved in Activation of Transcription. Mol. Cell. Biol. 24: 2153-2168 [Abstract] [Full Text]  
• McBryant, S. J., Park, Y.-J., Abernathy, S. M., Laybourn, P. J., Nyborg, J. K., Luger, K. (2003). Preferential Binding of the Histone (H3-H4)2 Tetramer by NAP1 Is Mediated by the Amino-terminal Histone Tails. J. Biol. Chem. 278: 44574-44583 [Abstract] [Full Text]  
• Tsuda, M., Takahashi, S., Takahashi, Y., Asahara, H. (2003). Transcriptional Co-activators CREB-binding Protein and p300 Regulate Chondrocyte-specific Gene Expression via Association with Sox9. J. Biol. Chem. 278: 27224-27229 [Abstract] [Full Text]  
• Georges, S. A., Giebler, H. A., Cole, P. A., Luger, K., Laybourn, P. J., Nyborg, J. K. (2003). Tax Recruitment of CBP/p300, via the KIX Domain, Reveals a Potent Requirement for Acetyltransferase Activity That Is Chromatin Dependent and Histone Tail Independent. Mol. Cell. Biol. 23: 3392-3404 [Abstract] [Full Text]  
• Smith, R. J., Dean, W., Konfortova, G., Kelsey, G. (2003). Identification of Novel Imprinted Genes in a Genome-Wide Screen for Maternal Methylation. Genome Res 13: 558-569 [Abstract] [Full Text]  
• Tenbrock, K., Juang, Y.-T., Tolnay, M., Tsokos, G. C. (2003). The Cyclic Adenosine 5'-Monophosphate Response Element Modulator Suppresses IL-2 Production in Stimulated T Cells by a Chromatin-Dependent Mechanism. J. Immunol. 170: 2971-2976 [Abstract] [Full Text]