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Molecular and Cellular Biology, May 2002, p. 3078-3088, Vol. 22, No. 9
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.9.3078-3088.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

The Mnd1 Protein Forms a Complex with Hop2 To Promote Homologous Chromosome Pairing and Meiotic Double-Strand Break Repair

Hideo Tsubouchi1 and G. Shirleen Roeder1,2*

Howard Hughes Medical Institute and Department of Molecular, Cellular and Developmental Biology,1 Department of Genetics, Yale University, New Haven, Connecticut 06520-81032

Received 27 November 2001/ Returned for modification 21 December 2001/ Accepted 30 January 2002

The hop2 mutant of Saccharomyces cerevisiae arrests in meiosis with extensive synaptonemal complex (SC) formation between nonhomologous chromosomes. A screen for multicopy suppressors of a hop2-ts allele identified the MND1 gene. The mnd1-null mutant arrests in meiotic prophase, with most double-strand breaks (DSBs) unrepaired. A low level of mature recombinants is produced, and the Rad51 protein accumulates at numerous foci along chromosomes. SC formation is incomplete, and homolog pairing is severely reduced. The Mnd1 protein localizes to chromatin throughout meiotic prophase, and this localization requires Hop2. Unlike recombination enzymes such as Rad51, Mnd1 localizes to chromosomes even in mutants that fail to initiate meiotic recombination. The Hop2 and Mnd1 proteins coimmunoprecipitate from meiotic cell extracts. These results suggest that Hop2 and Mnd1 work as a complex to promote meiotic chromosome pairing and DSB repair. The identification of Hop2 and Mnd1 homologs in other organisms suggests that the function of this complex is conserved among eukaryotes.

* Corresponding author. Mailing address: Howard Hughes Medical Institute, Department of Molecular, Cellular, and Developmental Biology, Yale University, P.O. Box 208103, New Haven, CT 06520-8103. Phone: (203) 432-3501. Fax: (203) 432-3263. E-mail: shirleen.roeder{at}yale.edu.

Molecular and Cellular Biology, May 2002, p. 3078-3088, Vol. 22, No. 9
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.9.3078-3088.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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