This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hoffman, L. M.
Right arrow Articles by Beckerle, M. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hoffman, L. M.
Right arrow Articles by Beckerle, M. C.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, January 2003, p. 70-79, Vol. 23, No. 1
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.1.70-79.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Targeted Disruption of the Murine zyxin Gene

Laura M. Hoffman,1 David A. Nix,1 Beverly Benson,1 Ray Boot-Hanford,2 Erika Gustafsson,2 Colin Jamora,3 A. Sheila Menzies,4 Keow Lin Goh,4 Christopher C. Jensen,1 Frank B. Gertler,4 Elaine Fuchs,3 Reinhard Fässler,2,1 and Mary C. Beckerle1*

Huntsman Cancer Institute and Department of Biology, University of Utah, Salt Lake City, Utah 84112,1 Department of Experimental Pathology, Lund University, 221 85 Lund, Sweden,2 Department of Molecular Genetics and Cell Biology and Howard Hughes Medical Institute, University of Chicago, Chicago, Illinois 60637,3 Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 021394

Received 8 July 2002/ Returned for modification 15 August 2002/ Accepted 27 September 2002

Zyxin is an evolutionarily conserved protein that is concentrated at sites of cell adhesion, where it associates with members of the Enabled (Ena)/vasodilator-stimulated phosphoprotein (VASP) family of cytoskeletal regulators and is postulated to play a role in cytoskeletal dynamics and signaling. Zyxin transcripts are detected throughout murine embryonic development, and the protein is widely expressed in adults. Here we used a reverse genetic approach to examine the consequences of loss of zyxin function in the mouse. Mice that lack zyxin function are viable and fertile and display no obvious histological abnormalities in any of the organs examined. Because zyxin contributes to the localization of Ena/VASP family members at certain subcellular locations, we carefully examined the zyxin-/- mice for evidence of defects that have been observed when Ena/VASP proteins are compromised in the mouse. Specifically, we evaluated blood platelet function, nervous system development, and skin architecture but did not detect any defects in these systems. Zyxin is the founding member of a family of proteins that also includes the lipoma preferred partner (LPP) and thyroid receptor-interacting protein 6 (TRIP6). These zyxin family members display patterns of expression that significantly overlap that of zyxin. Western blot analysis indicates that there is no detectable upregulation of either LPP or TRIP6 expression in tissues derived from zyxin-null mice. Because zyxin family members may have overlapping functions, a comprehensive understanding of the role of these proteins in the mouse will require the generation of compound mutations in which multiple zyxin family members are simultaneously compromised.

* Corresponding author. Mailing address: Huntsman Cancer Institute, University of Utah, 2000 Circle of Hope, Salt Lake City, UT 84112-5550. Phone: (801) 581-4485. Fax: (801) 581-2175. E-mail: mary.beckerle{at}hci.utah.edu.

{dagger} Present address: Department of Molecular Medicine, Max Planck Institute for Biochemistry, 82152 Martinsreid, Germany.

Molecular and Cellular Biology, January 2003, p. 70-79, Vol. 23, No. 1
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.1.70-79.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mori, M., Nakagami, H., Koibuchi, N., Miura, K., Takami, Y., Koriyama, H., Hayashi, H., Sabe, H., Mochizuki, N., Morishita, R., Kaneda, Y. (2009). Zyxin Mediates Actin Fiber Reorganization in Epithelial-Mesenchymal Transition and Contributes to Endocardial Morphogenesis. Mol. Biol. Cell 20: 3115-3124 [Abstract] [Full Text]  
  • Risebro, C. A., Searles, R. G., Melville, A. A. D., Ehler, E., Jina, N., Shah, S., Pallas, J., Hubank, M., Dillard, M., Harvey, N. L., Schwartz, R. J., Chien, K. R., Oliver, G., Riley, P. R. (2009). Prox1 maintains muscle structure and growth in the developing heart. Development 136: 495-505 [Abstract] [Full Text]  
  • LaFramboise, W. A., Jayaraman, R. C., Bombach, K. L., Ankrapp, D. P., Krill-Burger, J. M., Sciulli, C. M., Petrosko, P., Wiseman, R. W. (2009). Acute molecular response of mouse hindlimb muscles to chronic stimulation. Am. J. Physiol. Cell Physiol. 297: C556-C570 [Abstract] [Full Text]  
  • Takizawa, N., Smith, T. C., Nebl, T., Crowley, J. L., Palmieri, S. J., Lifshitz, L. M., Ehrhardt, A. G., Hoffman, L. M., Beckerle, M. C., Luna, E. J. (2006). Supervillin modulation of focal adhesions involving TRIP6/ZRP-1. JCB 174: 447-458 [Abstract] [Full Text]  
  • Hansen, M. D. H., Beckerle, M. C. (2006). Opposing Roles of Zyxin/LPP ACTA Repeats and the LIM Domain Region in Cell-Cell Adhesion. J. Biol. Chem. 281: 16178-16188 [Abstract] [Full Text]  
  • Brancaccio, M., Hirsch, E., Notte, A., Selvetella, G., Lembo, G., Tarone, G. (2006). Integrin signalling: The tug-of-war in heart hypertrophy. Cardiovasc Res 70: 422-433 [Abstract] [Full Text]  
  • Hoffman, L. M., Jensen, C. C., Kloeker, S., Wang, C.-L. A., Yoshigi, M., Beckerle, M. C. (2006). Genetic ablation of zyxin causes Mena/VASP mislocalization, increased motility, and deficits in actin remodeling. JCB 172: 771-782 [Abstract] [Full Text]  
  • Yoshigi, M., Hoffman, L. M., Jensen, C. C., Yost, H. J., Beckerle, M. C. (2005). Mechanical force mobilizes zyxin from focal adhesions to actin filaments and regulates cytoskeletal reinforcement. JCB 171: 209-215 [Abstract] [Full Text]  
  • Lai, Y.-J., Chen, C.-S., Lin, W.-C., Lin, F.-T. (2005). c-Src-Mediated Phosphorylation of TRIP6 Regulates Its Function in Lysophosphatidic Acid-Induced Cell Migration. Mol. Cell. Biol. 25: 5859-5868 [Abstract] [Full Text]  
  • Kisseleva, M., Feng, Y., Ward, M., Song, C., Anderson, R. A., Longmore, G. D. (2005). The LIM Protein Ajuba Regulates Phosphatidylinositol 4,5-Bisphosphate Levels in Migrating Cells through an Interaction with and Activation of PIPKI{alpha}. Mol. Cell. Biol. 25: 3956-3966 [Abstract] [Full Text]  
  • Lee, N. P.Y., Cheng, C. Y. (2004). Adaptors, Junction Dynamics, and Spermatogenesis. Biol. Reprod. 71: 392-404 [Abstract] [Full Text]  
  • Hamatani, T., Daikoku, T., Wang, H., Matsumoto, H., Carter, M. G., Ko, M. S. H., Dey, S. K. (2004). Global gene expression analysis identifies molecular pathways distinguishing blastocyst dormancy and activation. Proc. Natl. Acad. Sci. USA 101: 10326-10331 [Abstract] [Full Text]  
  • Conley, B. A., Koleva, R., Smith, J. D., Kacer, D., Zhang, D., Bernabeu, C., Vary, C. P. H. (2004). Endoglin Controls Cell Migration and Composition of Focal Adhesions: FUNCTION OF THE CYTOSOLIC DOMAIN. J. Biol. Chem. 279: 27440-27449 [Abstract] [Full Text]  
  • Stofega, M. R., Sanders, L. C., Gardiner, E. M., Bokoch, G. M. (2004). Constitutive p21-activated Kinase (PAK) Activation in Breast Cancer Cells as a Result of Mislocalization of PAK to Focal Adhesions. Mol. Biol. Cell 15: 2965-2977 [Abstract] [Full Text]  
  • Zimmermann, J., Kuhne, R., Volkmer-Engert, R., Jarchau, T., Walter, U., Oschkinat, H., Ball, L. J. (2003). Design of N-substituted Peptomer Ligands for EVH1 Domains. J. Biol. Chem. 278: 36810-36818 [Abstract] [Full Text]  
  • Nelander, S., Mostad, P., Lindahl, P. (2003). Prediction of Cell Type-Specific Gene Modules: Identification and Initial Characterization of a Core Set of Smooth Muscle-Specific Genes. Genome Res 13: 1838-1854 [Abstract] [Full Text]  
  • Garvalov, B. K., Higgins, T. E., Sutherland, J. D., Zettl, M., Scaplehorn, N., Kocher, T., Piddini, E., Griffiths, G., Way, M. (2003). The conformational state of Tes regulates its zyxin-dependent recruitment to focal adhesions. JCB 161: 33-39 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»