This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Georges, S. A. Articles by Nyborg, J. K. Search for Related Content PubMed PubMed Citation Articles by Georges, S. A. Articles by Nyborg, J. K.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, May 2003, p. 3392-3404, Vol. 23, No. 10
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.10.3392-3404.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Tax Recruitment of CBP/p300, via the KIX Domain, Reveals a Potent Requirement for Acetyltransferase Activity That Is Chromatin Dependent and Histone Tail Independent

Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523-1870,¹ Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205²

Received 21 October 2002/ Returned for modification 3 December 2002/ Accepted 4 March 2003

Robust transcription of human T-cell leukemia virus type 1 (HTLV-1) genome requires the viral transactivator Tax. Although Tax has been previously shown to interact with the KIX domain of CBP/p300 in vitro, the precise functional relevance of this interaction remains unclear. Using two distinct approaches to interrupt the physical interaction between Tax and KIX, we find that Tax transactivation from chromatin templates is strongly dependent on CBP/p300 recruitment via the KIX domain. Additionally, we find that the primary functional contribution of CBP/p300 to Tax transactivation resides in the intrinsic acetyltransferase activity of the coactivators. These studies unexpectedly uncover a specific requirement for CBP/p300 acetyltransferase activity on chromatin templates assembled with nucleosomes lacking their amino-terminal tails. Together, these data indicate that the KIX domain of CBP/p300 is essential for targeting the acetyltransferase activity of the coactivator to the Tax-CREB (Tax/CREB) complex. Significantly, these observations reveal the presence of one or more CBP/p300 acetyltransferase targets that function specifically on chromatin templates, are independent of the histone tails, and are critical to Tax transactivation.

This article has been cited by other articles:

• Sharma, N., Nyborg, J. K. (2008). The coactivators CBP/p300 and the histone chaperone NAP1 promote transcription-independent nucleosome eviction at the HTLV-1 promoter. Proc. Natl. Acad. Sci. USA 105: 7959-7963 [Abstract] [Full Text]  
• Geiger, T. R., Sharma, N., Kim, Y.-M., Nyborg, J. K. (2008). The Human T-Cell Leukemia Virus Type 1 Tax Protein Confers CBP/p300 Recruitment and Transcriptional Activation Properties to Phosphorylated CREB. Mol. Cell. Biol. 28: 1383-1392 [Abstract] [Full Text]  
• Merimi, M., Klener, P., Szynal, M., Cleuter, Y., Kerkhofs, P., Burny, A., Martiat, P., Van den Broeke, A. (2007). Suppression of Viral Gene Expression in Bovine Leukemia Virus-Associated B-Cell Malignancy: Interplay of Epigenetic Modifications Leading to Chromatin with a Repressive Histone Code. J. Virol. 81: 5929-5939 [Abstract] [Full Text]  
• Wencker, M., Sausse, C., Derse, D., Gazzolo, L., Duc Dodon, M. (2007). Human T-Cell Leukemia Virus Type 1 Tax Protein Down-Regulates Pre-T-Cell Receptor Alpha Gene Transcription in Human Immature Thymocytes. J. Virol. 81: 301-308 [Abstract] [Full Text]  
• Konesky, K. L., Nyborg, J. K., Laybourn, P. J. (2006). Tax Abolishes Histone H1 Repression of p300 Acetyltransferase Activity at the Human T-Cell Leukemia Virus Type 1 Promoter. J. Virol. 80: 10542-10553 [Abstract] [Full Text]  
• Tosi, G., Pilotti, E., Mortara, L., Barbaro, A. D. L., Casoli, C., Accolla, R. S. (2006). Inhibition of human T cell leukemia virus type 2 replication by the suppressive action of class II transactivator and nuclear factor Y. Proc. Natl. Acad. Sci. USA 103: 12861-12866 [Abstract] [Full Text]  
• Lemasson, I., Polakowski, N. J., Laybourn, P. J., Nyborg, J. K. (2006). Tax-dependent Displacement of Nucleosomes during Transcriptional Activation of Human T-Cell Leukemia Virus Type 1. J. Biol. Chem. 281: 13075-13082 [Abstract] [Full Text]  
• Tabakin-Fix, Y., Azran, I., Schavinky-Khrapunsky, Y., Levy, O., Aboud, M. (2006). Functional inactivation of p53 by human T-cell leukemia virus type 1 Tax protein: mechanisms and clinical implications. Carcinogenesis 27: 673-681 [Abstract] [Full Text]  
• Younis, I., Boris-Lawrie, K., Green, P. L. (2006). Human T-Cell Leukemia Virus Open Reading Frame II Encodes a Posttranscriptional Repressor That Is Recruited at the Level of Transcription. J. Virol. 80: 181-191 [Abstract] [Full Text]  
• Lemasson, I., Polakowski, N. J., Laybourn, P. J., Nyborg, J. K. (2004). Transcription Regulatory Complexes Bind the Human T-Cell Leukemia Virus 5' and 3' Long Terminal Repeats To Control Gene Expression. Mol. Cell. Biol. 24: 6117-6126 [Abstract] [Full Text]  
• Lu, H., Pise-Masison, C. A., Linton, R., Park, H. U., Schiltz, R. L., Sartorelli, V., Brady, J. N. (2004). Tax Relieves Transcriptional Repression by Promoting Histone Deacetylase 1 Release from the Human T-Cell Leukemia Virus Type 1 Long Terminal Repeat. J. Virol. 78: 6735-6743 [Abstract] [Full Text]  
• Livengood, J. A., Nyborg, J. K. (2004). The high-affinity Sp1 binding site in the HTLV-1 promoter contributes to Tax-independent basal expression. Nucleic Acids Res 32: 2829-2837 [Abstract] [Full Text]