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Molecular and Cellular Biology, May 2003, p. 3527-3535, Vol. 23, No. 10
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.10.3527-3535.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Functional Relation among RecQ Family Helicases RecQL1, RecQL5, and BLM in Cell Growth and Sister Chromatid Exchange Formation

Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578,¹ Cell Biology Laboratory, School of Pharmaceutical Sciences, Kinki University, Osaka 577-8502,² Department of Medical Genetics (Radiation Biology) B4, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan³

Received 28 June 2002/ Returned for modification 15 August 2002/ Accepted 31 January 2003

Human RECQL1 and RECQL5 belong to the RecQ family that includes Bloom syndrome, Werner syndrome, and Rothmund-Thomson syndrome causative genes. Cells derived from individuals suffering from these syndromes show significant levels of genomic instability. However, neither RECQL1 nor RECQL5 has been related to a disease, and nothing is known about the functions of RecQL1 and RecQL5. We generated here RECQL1^-/-, RECQL5^-/-, RECQL1^-/-/RECQL5^-/-, RECQL1^-/-/BLM^-/-, and RECQL5^-/-/BLM^-/- cells from chicken B-lymphocyte line DT40 cells. Although BLM^-/- DT40 cells showed a slow-growth phenotype, a higher sensitivity to methyl methanesulfonate than the wild type, and an 10-fold increase in the frequency of sister chromatid exchange (SCE) compared to wild-type cells, RECQL1^-/-, RECQL5^-/-, and RECQL1^-/-/RECQL5^-/- cells showed no significant difference from the wild-type cells in growth, sensitivity to DNA-damaging agents, and the frequency of SCE. However, both RECQL1^-/-/BLM^-/- and RECQL5^-/-/BLM^-/- cells grew more slowly than BLM^-/- cells because of the increase in the population of dead cells, indicating that RecQL1 and RecQL5 are somehow involved in cell viability under the BLM function-impaired condition. Surprisingly, RECQL5^-/-/BLM^-/- cells showed a higher frequency of SCE than BLM^-/- cells, indicating that RecQL5 suppresses SCE under the BLM function-impaired condition.

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