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Molecular and Cellular Biology, May 2003, p. 3527-3535, Vol. 23, No. 10
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.10.3527-3535.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Functional Relation among RecQ Family Helicases RecQL1, RecQL5, and BLM in Cell Growth and Sister Chromatid Exchange Formation

Wensheng Wang,1 Masayuki Seki,1* Yoshiyasu Narita,1 Takayuki Nakagawa,1 Akari Yoshimura,1 Makoto Otsuki,1 Yoh-ichi Kawabe,1 Shusuke Tada,1 Hideki Yagi,1,2 Yutaka Ishii,3 and Takemi Enomoto1

Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578,1 Cell Biology Laboratory, School of Pharmaceutical Sciences, Kinki University, Osaka 577-8502,2 Department of Medical Genetics (Radiation Biology) B4, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan3

Received 28 June 2002/ Returned for modification 15 August 2002/ Accepted 31 January 2003

Human RECQL1 and RECQL5 belong to the RecQ family that includes Bloom syndrome, Werner syndrome, and Rothmund-Thomson syndrome causative genes. Cells derived from individuals suffering from these syndromes show significant levels of genomic instability. However, neither RECQL1 nor RECQL5 has been related to a disease, and nothing is known about the functions of RecQL1 and RecQL5. We generated here RECQL1-/-, RECQL5-/-, RECQL1-/-/RECQL5-/-, RECQL1-/-/BLM-/-, and RECQL5-/-/BLM-/- cells from chicken B-lymphocyte line DT40 cells. Although BLM-/- DT40 cells showed a slow-growth phenotype, a higher sensitivity to methyl methanesulfonate than the wild type, and an ~10-fold increase in the frequency of sister chromatid exchange (SCE) compared to wild-type cells, RECQL1-/-, RECQL5-/-, and RECQL1-/-/RECQL5-/- cells showed no significant difference from the wild-type cells in growth, sensitivity to DNA-damaging agents, and the frequency of SCE. However, both RECQL1-/-/BLM-/- and RECQL5-/-/BLM-/- cells grew more slowly than BLM-/- cells because of the increase in the population of dead cells, indicating that RecQL1 and RecQL5 are somehow involved in cell viability under the BLM function-impaired condition. Surprisingly, RECQL5-/-/BLM-/- cells showed a higher frequency of SCE than BLM-/- cells, indicating that RecQL5 suppresses SCE under the BLM function-impaired condition.

* Corresponding author. Mailing address: Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba-ku, Sendai 980-8578, Japan. Phone: (81) 22-217-6875. Fax: (81) 22-217-6873. E-mail: seki{at}mail.pharm.tohoku.ac.jp.

Molecular and Cellular Biology, May 2003, p. 3527-3535, Vol. 23, No. 10
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.10.3527-3535.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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