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Molecular and Cellular Biology, May 2003, p. 3681-3691, Vol. 23, No. 10
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.10.3681-3691.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Telomeric Position Effect Variegation in Saccharomyces cerevisiae by Caenorhabditis elegans Linker Histones Suggests a Mechanistic Connection between Germ Line and Telomeric Silencing

Monika A. Jedrusik and Ekkehard Schulze*

Georg-August University of Göttingen, Third Department of Zoology-Developmental Biology, 37073 Göttingen, Germany

Received 20 December 2002/ Accepted 25 February 2003

Linker histones are nonessential for the life of single-celled eukaryotes. Linker histones, however, can be important components of specific developmental programs in multicellular animals and plants. For Caenorhabditis elegans a single linker histone variant (H1.1) is essential in a chromatin silencing process which is crucial for the proliferation and differentiation of the hermaphrodite germ line. In this study we analyzed the whole linker histone complement of C. elegans by telomeric position effect variegation in budding yeast. In this assay an indicator gene (URA3) placed close to the repressive telomeric chromatin structure is subject to epigenetically inherited gene inactivation. Just one out of seven C. elegans linker histones (H1.1) was able to enhance the telomeric position effect in budding yeast. Since these results reflect the biological function of H1.1 in C. elegans, we suggest that chromatin silencing in C. elegans is governed by molecular mechanisms related to the telomere-dependent silencing in budding yeast. We confirmed this hypothesis by testing C. elegans homologs of three yeast genes which are established modifiers of the yeast telomeric chromatin structure (SIR2, SET1, and RAD17) for their influence on repeat-dependent transgene silencing for C. elegans.

* Corresponding author. Mailing address: Third Dept. of Zoology-Developmental Biology, University of Göttingen, Humboldtallee 34A, D-37073 Göttingen, Germany. Phone: (49) 551 395426. Fax: (49) 551 395416. E-mail: eschulz{at}gwdg.de.

Molecular and Cellular Biology, May 2003, p. 3681-3691, Vol. 23, No. 10
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.10.3681-3691.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Wirth, M., Paap, F., Fischle, W., Wenzel, D., Agafonov, D. E., Samatov, T. R., Wisniewski, J. R., Jedrusik-Bode, M. (2009). HIS-24 Linker Histone and SIR-2.1 Deacetylase Induce H3K27me3 in the Caenorhabditis elegans Germ Line. Mol. Cell. Biol. 29: 3700-3709 [Abstract] [Full Text]  
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