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Molecular and Cellular Biology, June 2003, p. 3965-3973, Vol. 23, No. 11
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.11.3965-3973.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Cohesins Determine the Attachment Manner of Kinetochores to Spindle Microtubules at Meiosis I in Fission Yeast

Shihori Yokobayashi,1 Masayuki Yamamoto,1 and Yoshinori Watanabe1,2*

Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo,1 SORST, Japan Science and Technology Corporation, Hongo, Tokyo 113-0033, Japan2

Received 13 December 2002/ Returned for modification 13 February 2003/ Accepted 10 March 2003

During mitosis, sister kinetochores attach to microtubules that extend to opposite spindle poles (bipolar attachment) and pull the chromatids apart at anaphase (equational segregation). A multisubunit complex called cohesin, including Rad21/Scc1, plays a crucial role in sister chromatid cohesion and equational segregation at mitosis. Meiosis I differs from mitosis in having a reductional pattern of chromosome segregation, in which sister kinetochores are attached to the same spindle (monopolar attachment). During meiosis, Rad21/Scc1 is largely replaced by its meiotic counterpart, Rec8. If Rec8 is inactivated in fission yeast, meiosis I is shifted from reductional to equational division. However, the reason rec8{Delta} cells undergo equational rather than random division has not been clarified; therefore, it has been unclear whether equational segregation is due to a loss of cohesin in general or to a loss of a specific requirement for Rec8. We report here that the equational segregation at meiosis I depends on substitutive Rad21, which relocates to the centromeres if Rec8 is absent. Moreover, we demonstrate that even if sufficient amounts of Rad21 are transferred to the centromeres at meiosis I, thereby establishing cohesion at the centromeres, rec8{Delta} cells never recover monopolar attachment but instead secure bipolar attachment. Thus, Rec8 and Rad21 define monopolar and bipolar attachment, respectively, at meiosis I. We conclude that cohesin is a crucial determinant of the attachment manner of kinetochores to the spindle microtubules at meiosis I in fission yeast.

* Corresponding author. Mailing address: Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan. Phone: 81-3-5841-4387. Fax: 81-3-5802-2042. E-mail: ywatanab{at}ims.u-tokyo.ac.jp.

Molecular and Cellular Biology, June 2003, p. 3965-3973, Vol. 23, No. 11
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.11.3965-3973.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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