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Molecular and Cellular Biology, July 2003, p. 5064-5077, Vol. 23, No. 14
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.14.5064-5077.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

G1 Transcription Factors Are Differentially Regulated in Saccharomyces cerevisiae by the Swi6-Binding Protein Stb1

Michael Costanzo, Oliver Schub, and Brenda Andrews*

Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8

Received 10 February 2003/ Returned for modification 14 March 2003/ Accepted 10 April 2003

Stage-specific transcriptional programs are an integral feature of cell cycle regulation. In the budding yeast Saccharomyces cerevisiae, over 120 genes are coordinately induced in late G1 phase by two heterodimeric transcription factors called SBF and MBF. Activation of SBF and MBF is an upstream initiator of key cell cycle events, including budding and DNA replication. SBF and MBF regulation is complex and genetically redundant, and the precise mechanism of G1 transcriptional activation is unclear. Assays using SBF- and MBF-specific reporter genes revealed that the STB1 gene specifically affected MBF-dependent transcription. STB1 encodes a known Swi6-binding protein, but an MBF-specific function had not been previously suspected. Consistent with a specific role in regulating MBF, a STB1 deletion strain requires SBF for viability and microarray studies show a decrease in MBF-regulated transcripts in a swi4{Delta} mutant following depletion of Stb1. Chromatin immunoprecipitation experiments confirm that Stb1 localizes to promoters of MBF-regulated genes. Our data indicate that, contrary to previous models, MBF and SBF have unique components and might be distinctly regulated.

* Corresponding author. Mailing address: Department of Medical Genetics & Microbiology, University of Toronto, Rm. 4287, Medical Sciences Building, 1 King's College Cir., Toronto, ON M5S 1A8, Canada. Phone: 416-978-8562. Fax: 416-971-2494. E-mail: brenda.andrews{at}utoronto.ca.

Molecular and Cellular Biology, July 2003, p. 5064-5077, Vol. 23, No. 14
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.14.5064-5077.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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