This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tomita, K.
Right arrow Articles by Ueno, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tomita, K.
Right arrow Articles by Ueno, M.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, August 2003, p. 5186-5197, Vol. 23, No. 15
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.15.5186-5197.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Competition between the Rad50 Complex and the Ku Heterodimer Reveals a Role for Exo1 in Processing Double-Strand Breaks but Not Telomeres

Kazunori Tomita,1 Akira Matsuura,2 Thomas Caspari,3 Antony M. Carr,3 Yufuko Akamatsu,4 Hiroshi Iwasaki,4 Ken-ichi Mizuno,5 Kunihiro Ohta,5 Masahiro Uritani,1 Takashi Ushimaru,6 Koichi Yoshinaga,1 and Masaru Ueno1*

Department of Chemistry,1 Department of Biology, Shizuoka University, Shizuoka 422-8529,6 Department of Geriatric Research, National Institute for Longevity Science, Morioka, Obu, Aichi 474-8522,2 Graduate School of Integrated Science, Yokohama City University, Tsurumi-ku, Yokohama, Kanagawa 230-0045,4 Genetic Dynamics Research Unit Laboratory, RIKEN Institute, Wako, Saitama 351-0198, Japan,5 Genome Damage and Stability Centre, University of Sussex, Brighton BN19RQ, United Kingdom3

Received 30 September 2002/ Returned for modification 16 November 2002/ Accepted 8 May 2003

The Mre11-Rad50-Nbs1(Xrs2) complex and the Ku70-Ku80 heterodimer are thought to compete with each other for binding to DNA ends. To investigate the mechanism underlying this competition, we analyzed both DNA damage sensitivity and telomere overhangs in Schizosaccharomyces pombe rad50-d, rad50-d pku70-d, rad50-d exo1-d, and pku70-d rad50-d exo1-d cells. We found that rad50 exo1 double mutants are more methyl methanesulfonate (MMS) sensitive than the respective single mutants. The MMS sensitivity of rad50-d cells was suppressed by concomitant deletion of pku70+. However, the MMS sensitivity of the rad50 exo1 double mutant was not suppressed by the deletion of pku70+. The G-rich overhang at telomere ends in taz1-d cells disappeared upon deletion of rad50+, but the overhang reappeared following concomitant deletion of pku70+. Our data suggest that the Rad50 complex can process DSB ends and telomere ends in the presence of the Ku heterodimer. However, the Ku heterodimer inhibits processing of DSB ends and telomere ends by alternative nucleases in the absence of the Rad50-Rad32 protein complex. While we have identified Exo1 as the alternative nuclease targeting DNA break sites, the identity of the nuclease acting on the telomere ends remains elusive.

* Corresponding author. Mailing address: Department of Chemistry, Shizuoka University, 836 Oya, Shizuoka 422-8529, Japan. Phone: 81-54-238-4762. Fax: 81-54-237-3384. E-mail: scmueno{at}ipc.shizuoka.ac.jp.

Molecular and Cellular Biology, August 2003, p. 5186-5197, Vol. 23, No. 15
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.15.5186-5197.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Niu, H., Raynard, S., Sung, P. (2009). Multiplicity of DNA end resection machineries in chromosome break repair. Genes Dev. 23: 1481-1486 [Abstract] [Full Text]  
  • Farah, J. A., Cromie, G. A., Smith, G. R. (2009). Ctp1 and Exonuclease 1, alternative nucleases regulated by the MRN complex, are required for efficient meiotic recombination. Proc. Natl. Acad. Sci. USA 106: 9356-9361 [Abstract] [Full Text]  
  • Hartsuiker, E., Mizuno, K., Molnar, M., Kohli, J., Ohta, K., Carr, A. M. (2009). Ctp1CtIP and Rad32Mre11 Nuclease Activity Are Required for Rec12Spo11 Removal, but Rec12Spo11 Removal Is Dispensable for Other MRN-Dependent Meiotic Functions. Mol. Cell. Biol. 29: 1671-1681 [Abstract] [Full Text]  
  • Porter-Goff, M. E., Rhind, N. (2009). The Role of MRN in the S-Phase DNA Damage Checkpoint Is Independent of Its Ctp1-dependent Roles in Double-Strand Break Repair and Checkpoint Signaling. Mol. Biol. Cell 20: 2096-2107 [Abstract] [Full Text]  
  • Bechard, L. H., Butuner, B. D., Peterson, G. J., McRae, W., Topcu, Z., McEachern, M. J. (2009). Mutant Telomeric Repeats in Yeast Can Disrupt the Negative Regulation of Recombination-Mediated Telomere Maintenance and Create an Alternative Lengthening of Telomeres-Like Phenotype. Mol. Cell. Biol. 29: 626-639 [Abstract] [Full Text]  
  • Tomita, K., Cooper, J. P. (2008). Fission yeast Ccq1 is telomerase recruiter and local checkpoint controller. Genes Dev. 22: 3461-3474 [Abstract] [Full Text]  
  • Subramanian, L., Moser, B. A., Nakamura, T. M. (2008). Recombination-Based Telomere Maintenance Is Dependent on Tel1-MRN and Rap1 and Inhibited by Telomerase, Taz1, and Ku in Fission Yeast. Mol. Cell. Biol. 28: 1443-1455 [Abstract] [Full Text]  
  • Cullen, J. K., Hussey, S. P., Walker, C., Prudden, J., Wee, B.-Y., Dave, A., Findlay, J. S., Savory, A. P., Humphrey, T. C. (2007). Break-Induced Loss of Heterozygosity in Fission Yeast: Dual Roles for Homologous Recombination in Promoting Translocations and Preventing De Novo Telomere Addition. Mol. Cell. Biol. 27: 7745-7757 [Abstract] [Full Text]  
  • Heacock, M. L., Idol, R. A., Friesner, J. D., Britt, A. B., Shippen, D. E. (2007). Telomere dynamics and fusion of critically shortened telomeres in plants lacking DNA ligase IV. Nucleic Acids Res 35: 6490-6500 [Abstract] [Full Text]  
  • Martin, V., Du, L.-L., Rozenzhak, S., Russell, P. (2007). Protection of telomeres by a conserved Stn1 Ten1 complex. Proc. Natl. Acad. Sci. USA 104: 14038-14043 [Abstract] [Full Text]  
  • Ueno, K., Uno, J., Nakayama, H., Sasamoto, K., Mikami, Y., Chibana, H. (2007). Development of a Highly Efficient Gene Targeting System Induced by Transient Repression of YKU80 Expression in Candida glabrata. Eukaryot Cell 6: 1239-1247 [Abstract] [Full Text]  
  • Decottignies, A. (2007). Microhomology-Mediated End Joining in Fission Yeast Is Repressed by Pku70 and Relies on Genes Involved in Homologous Recombination. Genetics 176: 1403-1415 [Abstract] [Full Text]  
  • Kibe, T., Ono, Y., Sato, K., Ueno, M. (2007). Fission Yeast Taz1 and RPA Are Synergistically Required to Prevent Rapid Telomere Loss. Mol. Biol. Cell 18: 2378-2387 [Abstract] [Full Text]  
  • Lee, K. M., Nizza, S., Hayes, T., Bass, K. L., Irmisch, A., Murray, J. M., O'Connell, M. J. (2007). Brc1-Mediated Rescue of Smc5/6 Deficiency: Requirement for Multiple Nucleases and a Novel Rad18 Function. Genetics 175: 1585-1595 [Abstract] [Full Text]  
  • Sterling, C. H., Sweasy, J. B. (2006). DNA Polymerase 4 of Saccharomyces cerevisiae Is Important for Accurate Repair of Methyl-Methanesulfonate-Induced DNA Damage. Genetics 172: 89-98 [Abstract] [Full Text]  
  • Decottignies, A. (2005). Capture of Extranuclear DNA at Fission Yeast Double-Strand Breaks. Genetics 171: 1535-1548 [Abstract] [Full Text]  
  • Krogh, B. O., Llorente, B., Lam, A., Symington, L. S. (2005). Mutations in Mre11 Phosphoesterase Motif I That Impair Saccharomyces cerevisiae Mre11-Rad50-Xrs2 Complex Stability in Addition to Nuclease Activity. Genetics 171: 1561-1570 [Abstract] [Full Text]  
  • Kikuchi, K., Taniguchi, Y., Hatanaka, A., Sonoda, E., Hochegger, H., Adachi, N., Matsuzaki, Y., Koyama, H., van Gent, D. C., Jasin, M., Takeda, S. (2005). Fen-1 Facilitates Homologous Recombination by Removing Divergent Sequences at DNA Break Ends. Mol. Cell. Biol. 25: 6948-6955 [Abstract] [Full Text]  
  • Morikawa, H., Morishita, T., Kawane, S., Iwasaki, H., Carr, A. M., Shinagawa, H. (2004). Rad62 Protein Functionally and Physically Associates with the Smc5/Smc6 Protein Complex and Is Required for Chromosome Integrity and Recombination Repair in Fission Yeast. Mol. Cell. Biol. 24: 9401-9413 [Abstract] [Full Text]  
  • Tomita, K., Kibe, T., Kang, H.-Y., Seo, Y.-S., Uritani, M., Ushimaru, T., Ueno, M. (2004). Fission Yeast Dna2 Is Required for Generation of the Telomeric Single-Strand Overhang. Mol. Cell. Biol. 24: 9557-9567 [Abstract] [Full Text]  
  • Ferreira, M. G., Cooper, J. P. (2004). Two modes of DNA double-strand break repair are reciprocally regulated through the fission yeast cell cycle. Genes Dev. 18: 2249-2254 [Abstract] [Full Text]  
  • Zubko, M. K., Guillard, S., Lydall, D. (2004). Exo1 and Rad24 Differentially Regulate Generation of ssDNA at Telomeres of Saccharomyces cerevisiae cdc13-1 Mutants. Genetics 168: 103-115 [Abstract] [Full Text]  
  • Larrivee, M., LeBel, C., Wellinger, R. J. (2004). The generation of proper constitutive G-tails on yeast telomeres is dependent on the MRX complex. Genes Dev. 18: 1391-1396 [Abstract] [Full Text]  
  • Ono, Y., Tomita, K., Matsuura, A., Nakagawa, T., Masukata, H., Uritani, M., Ushimaru, T., Ueno, M. (2003). A novel allele of fission yeast rad11 that causes defects in DNA repair and telomere length regulation. Nucleic Acids Res 31: 7141-7149 [Abstract] [Full Text]  
  • Ueno, M., Nakazaki, T., Akamatsu, Y., Watanabe, K., Tomita, K., Lindsay, H. D., Shinagawa, H., Iwasaki, H. (2003). Molecular Characterization of the Schizosaccharomyces pombe nbs1+ Gene Involved in DNA Repair and Telomere Maintenance. Mol. Cell. Biol. 23: 6553-6563 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»