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Molecular and Cellular Biology, August 2003, p. 5502-5515, Vol. 23, No. 16
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.16.5502-5515.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Degradation of Normal mRNA in the Nucleus of Saccharomyces cerevisiae

Biswadip Das,1 J. Scott Butler,2 and Fred Sherman1*

Department of Biochemistry and Biophysics,1 Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York 146422

Received 10 December 2002/ Returned for modification 31 January 2003/ Accepted 20 May 2003

A nuclear mRNA degradation (DRN) system was identified from analysis of mRNA turnover rates in nup116-{Delta} strains of Saccharomyces cerevisiae lacking the ability to export all RNAs, including poly(A) mRNAs, at the restrictive temperature. Northern blotting, in situ hybridization, and blocking transcription with thiolutin in nup116-{Delta} strains revealed a rapid degradation of mRNAs in the nucleus that was suppressed by the rrp6-{Delta}, rai1-{Delta}, and cbc1-{Delta} deletions, but not by the upf1-{Delta} deletion, suggesting that DRN requires Rrp6p, a 3'-to-5' nuclear exonuclease, the Rat1p, a 5'-to-3' nuclear exonuclease, and Cbc1p, a component of CBC, the nuclear cap binding complex, which may direct the mRNAs to the site of degradation. We propose that certain normal mRNAs retained in the nucleus are degraded by the DRN system, similar to degradation of transcripts with 3' end formation defects in certain mutants.

* Corresponding author. Mailing address: Department of Biochemistry and Biophysics, Box 712, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642. Phone: (585) 275-6647. Fax: (585) 275-6007. E-mail: Fred_Sherman{at}urmc.rochester.edu.

Molecular and Cellular Biology, August 2003, p. 5502-5515, Vol. 23, No. 16
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.16.5502-5515.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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