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Molecular and Cellular Biology, August 2003, p. 5928-5938, Vol. 23, No. 16
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.16.5928-5938.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Support for a Meiotic Recombination Initiation Complex: Interactions among Rec102p, Rec104p, and Spo11p

Kai Jiao,^1, Laura Salem,^2, and Robert Malone^1,2*

Department of Biological Sciences,¹ Program in Genetics, University of Iowa, Iowa City, Iowa 52242²

Received 12 September 2002/ Returned for modification 25 October 2002/ Accepted 13 May 2003

Initiation of meiotic recombination in the yeast Saccharomyces cerevisiae requires at least 10 gene products. The initiation event creates double-strand breaks, which are then processed by other recombination enzymes. A variety of classical observations, such as the existence of recombination nodules, have suggested that the proteins catalyzing recombination form a complex. A variety of lines of evidence indicate that Rad50p, Mre11p, and Xrs2p interact, and genetic data suggesting interactions between Rec102p and Rec104p have been reported. It has recently been shown that Spo11p coimmunoprecipitates with Rec102p in meiosis as well. In this paper, we provide genetic and biochemical evidence that the meiosis-specific proteins Rec102p, Rec104p, and Spo11p all interact with each other in meiosis. Furthermore, we demonstrate that the interaction between Rec102p and Spo11p does not require Rec104p. Likewise, the interaction between Rec104p and Rec102p does not require Spo11p, although Spo11p may stabilize that association. The interactions suggest that Spo11p, Rec102p, and Rec104p may form a trimeric complex during the initiation of recombination.

Present address: Pediatric Cardiology, Vanderbilt University, Nashville, TN 37232.

Present address: School of Biological Sciences, University of Missouri—Kansas City, Kansas City, MO 64110.

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