Previous Article | Next Article 
Molecular and Cellular Biology, September 2003, p. 6150-6158, Vol. 23, No. 17
0270-7306/03/$08.00+0 DOI: 10.1128/MCB.23.17.6150-6158.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
Retention but Not Recruitment of Crb2 at Double-Strand Breaks Requires Rad1 and Rad3 Complexes
Li-Lin Du, Toru M. Nakamura, Bettina A. Moser, and Paul Russell*
Departments of Molecular Biology and Cell Biology, The Scripps Research Institute, La Jolla, California 92037
Received 30 April 2003/
Returned for modification 27 May 2003/
Accepted 5 June 2003
The fission yeast checkpoint protein Crb2, related to budding yeast Rad9 and human 53BP1 and BRCA1, has been suggested to act as an adapter protein facilitating the phosphorylation of specific substrates by Rad3-Rad26 kinase. To further understand its role in checkpoint signaling, we examined its localization in live cells by using fluorescence microscopy. In response to DNA damage, Crb2 localizes to distinct nuclear foci, which represent sites of DNA double-strand breaks (DSBs). Crb2 colocalizes with Rad22 at persistent foci, suggesting that Crb2 is retained at sites of DNA damage during repair. Damage-induced Crb2 foci still form in cells defective in Rad1, Rad3, and Rad17 complexes, but these foci do not persist as long as in wild-type cells. Our results suggest that Crb2 functions at the sites of DNA damage, and its regulated persistent localization at damage sites may be involved in facilitating DNA repair and/or maintaining the checkpoint arrest while DNA repair is under way.
* Corresponding author. Mailing address: The Scripps Research Institute, 10550 North Torrey Pines Rd., La Jolla, CA 92037. Phone: (858) 784-8273. Fax: (858) 784-2265. E-mail: prussell{at}scripps.edu.
Molecular and Cellular Biology, September 2003, p. 6150-6158, Vol. 23, No. 17
0022-538X/03/$08.00+0 DOI: 10.1128/MCB.23.17.6150-6158.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
This article has been cited by other articles:
-
Kilkenny, M. L., Dore, A. S., Roe, S. M., Nestoras, K., Ho, J. C.Y., Watts, F. Z., Pearl, L. H.
(2008). Structural and functional analysis of the Crb2-BRCT2 domain reveals distinct roles in checkpoint signaling and DNA damage repair. Genes Dev.
22: 2034-2047
[Abstract]
[Full Text]
-
Gomez, E. B., Nugent, R. L., Laria, S., Forsburg, S. L.
(2008). Schizosaccharomyces pombe Histone Acetyltransferase Mst1 (KAT5) Is an Essential Protein Required for Damage Response and Chromosome Segregation. Genetics
179: 757-771
[Abstract]
[Full Text]
-
Diaz-Cuervo, H., Bueno, A.
(2008). Cds1 Controls the Release of Cdc14-like Phosphatase Flp1 from the Nucleolus to Drive Full Activation of the Checkpoint Response to Replication Stress in Fission Yeast. Mol. Biol. Cell
19: 2488-2499
[Abstract]
[Full Text]
-
Bailis, J. M., Luche, D. D., Hunter, T., Forsburg, S. L.
(2008). Minichromosome Maintenance Proteins Interact with Checkpoint and Recombination Proteins To Promote S-Phase Genome Stability. Mol. Cell. Biol.
28: 1724-1738
[Abstract]
[Full Text]
-
Lancelot, N., Charier, G., Couprie, J., Duband-Goulet, I., Alpha-Bazin, B., Quemeneur, E., Ma, E., Marsolier-Kergoat, M.-C., Ropars, V., Charbonnier, J.-B., Miron, S., Craescu, C. T., Callebaut, I., Gilquin, B., Zinn-Justin, S.
(2007). The checkpoint Saccharomyces cerevisiae Rad9 protein contains a tandem tudor domain that recognizes DNA. Nucleic Acids Res
35: 5898-5912
[Abstract]
[Full Text]
-
Dovey, C. L., Russell, P.
(2007). Mms22 Preserves Genomic Integrity During DNA Replication in Schizosaccharomyces pombe. Genetics
177: 47-61
[Abstract]
[Full Text]
-
Kearsey, S. E., Stevenson, A. L., Toda, T., Wang, S.-W.
(2007). Fission Yeast Cut8 Is Required for the Repair of DNA Double-Strand Breaks, Ribosomal DNA Maintenance, and Cell Survival in the Absence of Rqh1 Helicase. Mol. Cell. Biol.
27: 1558-1567
[Abstract]
[Full Text]
-
Shikata, M., Ishikawa, F., Kanoh, J.
(2007). Tel2 Is Required for Activation of the Mrc1-mediated Replication Checkpoint. J. Biol. Chem.
282: 5346-5355
[Abstract]
[Full Text]
-
Grandin, N., Charbonneau, M.
(2007). Control of the yeast telomeric senescence survival pathways of recombination by the Mec1 and Mec3 DNA damage sensors and RPA. Nucleic Acids Res
35: 822-838
[Abstract]
[Full Text]
-
Noguchi, C., Noguchi, E.
(2007). Sap1 Promotes the Association of the Replication Fork Protection Complex With Chromatin and Is Involved in the Replication Checkpoint in Schizosaccharomyces pombe. Genetics
175: 553-566
[Abstract]
[Full Text]
-
Du, L.-L., Nakamura, T. M., Russell, P.
(2006). Histone modification-dependent and -independent pathways for recruitment of checkpoint protein Crb2 to double-strand breaks.. Genes Dev.
20: 1583-1596
[Abstract]
[Full Text]
-
Hope, J. C., Mense, S. M., Jalakas, M., Mitsumoto, J., Freyer, G. A.
(2006). Rqh1 blocks recombination between sister chromatids during double strand break repair, independent of its helicase activity. Proc. Natl. Acad. Sci. USA
103: 5875-5880
[Abstract]
[Full Text]
-
Coulon, S., Noguchi, E., Noguchi, C., Du, L.-L., Nakamura, T. M., Russell, P.
(2006). Rad22Rad52-dependent Repair of Ribosomal DNA Repeats Cleaved by Slx1-Slx4 Endonuclease. Mol. Biol. Cell
17: 2081-2090
[Abstract]
[Full Text]
-
Pebernard, S., Wohlschlegel, J., McDonald, W. H., Yates, J. R. III, Boddy, M. N.
(2006). The nse5-nse6 dimer mediates DNA repair roles of the smc5-smc6 complex.. Mol. Cell. Biol.
26: 1617-1630
[Abstract]
[Full Text]
-
Mochida, S., Yanagida, M.
(2006). Distinct modes of DNA damage response in S. pombe G0 and vegetative cells. GENES CELLS
11: 13-27
[Abstract]
[Full Text]
-
Matsumoto, S., Ogino, K., Noguchi, E., Russell, P., Masai, H.
(2005). Hsk1-Dfp1/Him1, the Cdc7-Dbf4 Kinase in Schizosaccharomyces pombe, Associates with Swi1, a Component of the Replication Fork Protection Complex. J. Biol. Chem.
280: 42536-42542
[Abstract]
[Full Text]
-
Nakamura, T. M., Moser, B. A., Du, L.-L., Russell, P.
(2005). Cooperative Control of Crb2 by ATM Family and Cdc2 Kinases Is Essential for the DNA Damage Checkpoint in Fission Yeast. Mol. Cell. Biol.
25: 10721-10730
[Abstract]
[Full Text]
-
You, Z., Chahwan, C., Bailis, J., Hunter, T., Russell, P.
(2005). ATM Activation and Its Recruitment to Damaged DNA Require Binding to the C Terminus of Nbs1. Mol. Cell. Biol.
25: 5363-5379
[Abstract]
[Full Text]
-
Pryde, F., Khalili, S., Robertson, K., Selfridge, J., Ritchie, A.-M., Melton, D. W., Jullien, D., Adachi, Y.
(2005). 53BP1 exchanges slowly at the sites of DNA damage and appears to require RNA for its association with chromatin. J. Cell Sci.
118: 2043-2055
[Abstract]
[Full Text]
-
Giannattasio, M., Lazzaro, F., Plevani, P., Muzi-Falconi, M.
(2005). The DNA Damage Checkpoint Response Requires Histone H2B Ubiquitination by Rad6-Bre1 and H3 Methylation by Dot1. J. Biol. Chem.
280: 9879-9886
[Abstract]
[Full Text]
-
Meister, P., Taddei, A., Vernis, L., Poidevin, M., Gasser, S. M., Baldacci, G.
(2005). Temporal separation of replication and recombination requires the intra-S checkpoint. J. Cell Biol.
168: 537-544
[Abstract]
[Full Text]
-
Jurvansuu, J., Raj, K., Stasiak, A., Beard, P.
(2005). Viral Transport of DNA Damage That Mimics a Stalled Replication Fork. J. Virol.
79: 569-580
[Abstract]
[Full Text]
-
O'Connell, M. J., Cimprich, K. A.
(2005). G2 damage checkpoints: what is the turn-on?. J. Cell Sci.
118: 1-6
[Abstract]
[Full Text]
-
Noguchi, E., Noguchi, C., McDonald, W. H., Yates, J. R. III, Russell, P.
(2004). Swi1 and Swi3 Are Components of a Replication Fork Protection Complex in Fission Yeast. Mol. Cell. Biol.
24: 8342-8355
[Abstract]
[Full Text]
-
Du, L.-L., Moser, B. A., Russell, P.
(2004). Homo-oligomerization Is the Essential Function of the Tandem BRCT Domains in the Checkpoint Protein Crb2. J. Biol. Chem.
279: 38409-38414
[Abstract]
[Full Text]
-
Nakamura, T. M., Du, L.-L., Redon, C., Russell, P.
(2004). Histone H2A Phosphorylation Controls Crb2 Recruitment at DNA Breaks, Maintains Checkpoint Arrest, and Influences DNA Repair in Fission Yeast. Mol. Cell. Biol.
24: 6215-6230
[Abstract]
[Full Text]
-
Furuya, K., Poitelea, M., Guo, L., Caspari, T., Carr, A. M.
(2004). Chk1 activation requires Rad9 S/TQ-site phosphorylation to promote association with C-terminal BRCT domains of Rad4TOPBP1. Genes Dev.
18: 1154-1164
[Abstract]
[Full Text]
-
Naiki, T., Wakayama, T., Nakada, D., Matsumoto, K., Sugimoto, K.
(2004). Association of Rad9 with Double-Strand Breaks through a Mec1-Dependent Mechanism. Mol. Cell. Biol.
24: 3277-3285
[Abstract]
[Full Text]
-
Noguchi, E., Noguchi, C., Du, L.-L., Russell, P.
(2003). Swi1 Prevents Replication Fork Collapse and Controls Checkpoint Kinase Cds1. Mol. Cell. Biol.
23: 7861-7874
[Abstract]
[Full Text]
Copyright © 2003 by the American Society for Microbiology. All rights reserved.