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Molecular and Cellular Biology, September 2003, p. 6327-6337, Vol. 23, No. 17
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.17.6327-6337.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Specific Inhibition of Elm1 Kinase Activity Reveals Functions Required for Early G1 Events

Aparna Sreenivasan,1 Anthony C. Bishop,2 Kevan M. Shokat,2 and Douglas R. Kellogg1*

Sinsheimer Laboratories, Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Cruz, California 95064,1 Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California 94143-04502

Received 10 March 2003/ Returned for modification 16 April 2003/ Accepted 10 June 2003

In budding yeast, the Elm1 kinase is required for coordination of cell growth and cell division at G2/M. Elm1 is also required for efficient cytokinesis and for regulation of Swe1, the budding yeast homolog of the Wee1 kinase. To further characterize Elm1 function, we engineered an ELM1 allele that can be rapidly and selectively inhibited in vivo. We found that inhibition of Elm1 kinase activity during G2 results in a phenotype similar to the phenotype caused by deletion of the ELM1 gene, as expected. However, inhibition of Elm1 kinase activity earlier in the cell cycle results in a prolonged G1 delay. The G1 requirement for Elm1 kinase activity occurs before bud emergence, polarization of the septins, and synthesis of G1 cyclins. Inhibition of Elm1 kinase activity during early G1 also causes defects in the organization of septins, and inhibition of Elm1 kinase activity in a strain lacking the redundant G1 cyclins CLN1 and CLN2 is lethal. These results demonstrate that the Elm1 kinase plays an important role in G1 events required for bud emergence and septin organization.

* Corresponding author. Mailing address: Sinsheimer Laboratories, Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Cruz, CA 95064. Phone: (831) 459-5659. Fax: (831) 459-3139. E-mail: kellogg{at}darwin.ucsc.edu.

Molecular and Cellular Biology, September 2003, p. 6327-6337, Vol. 23, No. 17
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.17.6327-6337.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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