This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Skryabin, B. V.
Right arrow Articles by Brosius, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Skryabin, B. V.
Right arrow Articles by Brosius, J.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, September 2003, p. 6435-6441, Vol. 23, No. 18
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.18.6435-6441.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Neuronal Untranslated BC1 RNA: Targeted Gene Elimination in Mice

Boris V. Skryabin,1 Valentina Sukonina,1,{dagger} Ursula Jordan,1 Lars Lewejohann,2 Norbert Sachser,2 Ilham Muslimov,3 Henri Tiedge,3 and Jürgen Brosius1*

Institute of Experimental Pathology,1 Department of Behavioral Biology, University of Münster, D-48149 Münster, Germany,2 Department of Physiology and Pharmacology, State University of New York Health Science Center at Brooklyn, Brooklyn, New York 112033

Received 21 February 2003/ Returned for modification 25 April 2003/ Accepted 16 June 2003

Despite the potentially important roles of untranslated RNAs in cellular form or function, genes encoding such RNAs have until now received surprisingly little attention. One such gene encodes BC1 RNA, a small non-mRNA that is delivered to dendritic microdomains in neurons. We have now eliminated the BC1 RNA gene in mice. Three independent founder lines were established from separate embryonic stem cells. The mutant mice appeared to be healthy and showed no anatomical or neurological abnormalities. The gross brain morphology was unaltered in such mice, as were the subcellular distributions of two prototypical dendritic mRNAs (encoding MAP2 and CaMKII{alpha}). Due to the relatively recent evolutionary origin of the gene, we expected molecular and behavioral consequences to be subtle. Behavioral analyses, to be reported separately, indicate that the lack of BC1 RNA appears to reduce exploratory activity.

* Corresponding author. Mailing address: Institute of Experimental Pathology (ZMBE), University of Münster, Von-Esmarch Str. 56, D-48149 Münster, Germany. Phone: 49-251-835 8511. Fax: 49-251-835 8512. E-mail: RNA.world{at}uni-muenster.de.

{dagger} Present address: Department of Medical Biosciences, University of Umea, 90187 Umea, Sweden.

Molecular and Cellular Biology, September 2003, p. 6435-6441, Vol. 23, No. 18
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.18.6435-6441.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Iacoangeli, A., Rozhdestvensky, T. S., Dolzhanskaya, N., Tournier, B., Schutt, J., Brosius, J., Denman, R. B., Khandjian, E. W., Kindler, S., Tiedge, H. (2008). On BC1 RNA and the fragile X mental retardation protein. Proc. Natl. Acad. Sci. USA 105: 734-739 [Abstract] [Full Text]  
  • Centonze, D., Rossi, S., Napoli, I., Mercaldo, V., Lacoux, C., Ferrari, F., Ciotti, M. T., De Chiara, V., Prosperetti, C., Maccarrone, M., Fezza, F., Calabresi, P., Bernardi, G., Bagni, C. (2007). The Brain Cytoplasmic RNA BC1 Regulates Dopamine D2 Receptor-Mediated Transmission in the Striatum. J. Neurosci. 27: 8885-8892 [Abstract] [Full Text]  
  • Khanam, T., Rozhdestvensky, T. S., Bundman, M., Galiveti, C. R., Handel, S., Sukonina, V., Jordan, U., Brosius, J., Skryabin, B. V. (2007). Two primate-specific small non-protein-coding RNAs in transgenic mice: neuronal expression, subcellular localization and binding partners. Nucleic Acids Res 35: 529-539 [Abstract] [Full Text]  
  • Wang, H., Iacoangeli, A., Lin, D., Williams, K., Denman, R. B., Hellen, C. U.T., Tiedge, H. (2005). Dendritic BC1 RNA in translational control mechanisms. JCB 171: 811-821 [Abstract] [Full Text]  
  • Reeder, J., Giegerich, R. (2005). Consensus shapes: an alternative to the Sankoff algorithm for RNA consensus structure prediction. Bioinformatics 21: 3516-3523 [Abstract] [Full Text]  
  • Smith, R. (2004). Moving Molecules: mRNA Trafficking in Mammalian Oligodendrocytes and Neurons. Neuroscientist 10: 495-500 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»