This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shulga, N.
Right arrow Articles by Goldfarb, D. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shulga, N.
Right arrow Articles by Goldfarb, D. S.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, January 2003, p. 534-542, Vol. 23, No. 2
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.2.534-542.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Binding Dynamics of Structural Nucleoporins Govern Nuclear Pore Complex Permeability and May Mediate Channel Gating

Nataliya Shulga and David S. Goldfarb*

Department of Biology, University of Rochester, Rochester, New York 14627

Received 7 August 2002/ Returned for modification 12 September 2002/ Accepted 4 October 2002

The nuclear pore complex (NPC) is a permeable sieve that can dilate to facilitate the bidirectional translocation of a wide size range of receptor-cargo complexes. The binding of receptors to FG nucleoporin docking sites triggers channel gating by an unknown mechanism. Previously, we used deoxyglucose and chilling treatments to implicate Nup170p and Nup188p in the control of NPC sieving in Saccharomyces cerevisiae. Here, we report that aliphatic alcohols increase the permeability of wild-type and nup170{Delta} NPCs. In conjunction with increases in permeability, aliphatic alcohols, deoxyglucose, and chilling trigger the reversible dissociation of several nucleoporins from nup170{Delta} NPCs. These results are consistent with the hypothesis that NPC gating occurs when molecular latches composed of FG repeats and structural nucleoporins dissociate.

* Corresponding author. Mailing address: Department of Biology, University of Rochester, Rochester, NY 14627. Phone: (585) 275-3890. Fax: (585) 275-2070. E-mail: dasg{at}mail.rochester.edu.

Molecular and Cellular Biology, January 2003, p. 534-542, Vol. 23, No. 2
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.2.534-542.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sun, C., Yang, W., Tu, L.-C., Musser, S. M. (2008). Single-molecule measurements of importin {alpha}/cargo complex dissociation at the nuclear pore. Proc. Natl. Acad. Sci. USA 105: 8613-8618 [Abstract] [Full Text]  
  • Patel, S. S., Rexach, M. F. (2008). Discovering Novel Interactions at the Nuclear Pore Complex Using Bead Halo: A Rapid Method for Detecting Molecular Interactions of High and Low Affinity at Equilibrium. Mol. Cell. Proteomics 7: 121-131 [Abstract] [Full Text]  
  • Terry, L. J., Shows, E. B., Wente, S. R. (2007). Crossing the Nuclear Envelope: Hierarchical Regulation of Nucleocytoplasmic Transport. Science 318: 1412-1416 [Abstract] [Full Text]  
  • Lim, R. Y. H., Fahrenkrog, B., Koser, J., Schwarz-Herion, K., Deng, J., Aebi, U. (2007). Nanomechanical Basis of Selective Gating by the Nuclear Pore Complex. Science 318: 640-643 [Abstract] [Full Text]  
  • Sistla, S., Pang, J. V., Wang, C. X., Balasundaram, D. (2007). Multiple Conserved Domains of the Nucleoporin Nup124p and Its Orthologs Nup1p and Nup153 Are Critical for Nuclear Import and Activity of the Fission Yeast Tf1 Retrotransposon. Mol. Biol. Cell 18: 3692-3708 [Abstract] [Full Text]  
  • Makhnevych, T., Ptak, C., Lusk, C. P., Aitchison, J. D., Wozniak, R. W. (2007). The role of karyopherins in the regulated sumoylation of septins. JCB 177: 39-49 [Abstract] [Full Text]  
  • Grimaldi, M. R., Cozzolino, L., Malva, C., Graziani, F., Gigliotti, S. (2007). nup154 Genetically Interacts With cup and Plays a Cell-Type-Specific Function During Drosophila melanogaster Egg-Chamber Development. Genetics 175: 1751-1759 [Abstract] [Full Text]  
  • Lim, R. Y. H., Huang, N.-P., Koser, J., Deng, J., Lau, K. H. A., Schwarz-Herion, K., Fahrenkrog, B., Aebi, U. (2006). Flexible phenylalanine-glycine nucleoporins as entropic barriers to nucleocytoplasmic transport. Proc. Natl. Acad. Sci. USA 103: 9512-9517 [Abstract] [Full Text]  
  • Shahin, V., Ludwig, Y., Schafer, C., Nikova, D., Oberleithner, H. (2005). Glucocorticoids remodel nuclear envelope structure and permeability. J. Cell Sci. 118: 2881-2889 [Abstract] [Full Text]  
  • Menon, B. B., Sarma, N. J., Pasula, S., Deminoff, S. J., Willis, K. A., Barbara, K. E., Andrews, B., Santangelo, G. M. (2005). Reverse recruitment: The Nup84 nuclear pore subcomplex mediates Rap1/Gcr1/Gcr2 transcriptional activation. Proc. Natl. Acad. Sci. USA 102: 5749-5754 [Abstract] [Full Text]  
  • Izawa, S., Takemura, R., Inoue, Y. (2004). Gle2p Is Essential to Induce Adaptation of the Export of Bulk Poly(A)+ mRNA to Heat Shock in Saccharomyces cerevisiae. J. Biol. Chem. 279: 35469-35478 [Abstract] [Full Text]  
  • Takemura, R., Inoue, Y., Izawa, S. (2004). Stress response in yeast mRNA export factor: reversible changes in Rat8p localization are caused by ethanol stress but not heat shock. J. Cell Sci. 117: 4189-4197 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»