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Molecular and Cellular Biology, November 2003, p. 7937-7946, Vol. 23, No. 22
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.22.7937-7946.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Replication-Independent Assembly of Nucleosome Arrays in a Novel Yeast Chromatin Reconstitution System Involves Antisilencing Factor Asf1p and Chromodomain Protein Chd1p

Karen M. Robinson and Michael C. Schultz*

Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7

Received 24 June 2003/ Returned for modification 21 July 2003/ Accepted 11 August 2003

Chromatin assembly in a crude DEAE (CD) fraction from budding yeast is ATP dependent and generates arrays of physiologically spaced nucleosomes which significantly protect constituent DNA from restriction endonuclease digestion. The CD fractions from mutants harboring deletions of the genes encoding histone-binding factors (NAP1, ASF1, and a subunit of CAF-I) and SNF2-like DEAD/H ATPases (SNF2, ISW1, ISW2, CHD1, SWR1, YFR038w, and SPT20) were screened for activity in this replication-independent system. ASF1 deletion substantially inhibits assembly, a finding consistent with published evidence that Asf1p is a chromatin assembly factor. Surprisingly, a strong assembly defect is also associated with deletion of CHD1, suggesting that like other SNF2-related groups of nucleic acid-stimulated ATPases, the chromodomain (CHD) group may contain a member involved in chromatin reconstitution. In contrast to the effects of disrupting ASF1 and CHD1, deletion of SNF2 is associated with increased resistance of chromatin to digestion by micrococcal nuclease. We discuss the possible implications of these findings for current understanding of the diversity of mechanisms by which chromatin reconstitution and remodeling can be achieved in vivo.

* Corresponding author. Mailing address: Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada. Phone: (780) 492-9144. Fax: (780) 492-9556. E-mail for Karen M. Robinson: karen.robinson{at}ualberta.ca. E-mail for Michael C. Schultz: michael.schultz{at}ualberta.ca.

Molecular and Cellular Biology, November 2003, p. 7937-7946, Vol. 23, No. 22
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.22.7937-7946.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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