This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Weber, M.
Right arrow Articles by Forné, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Weber, M.
Right arrow Articles by Forné, T.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, December 2003, p. 8953-8959, Vol. 23, No. 24
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.24.8953-8959.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Genomic Imprinting Controls Matrix Attachment Regions in the Igf2 Gene

Michaël Weber,1 Hélène Hagège,1 Adele Murrell,2 Claude Brunel,1 Wolf Reik,2 Guy Cathala,1 and Thierry Forné1*

Institut de Génétique Moléculaire, UMR 5535 CNRS, Université Montpellier II, IFR 122, 34293 Montpellier Cedex 5, France,1 Laboratory of Developmental Genetics and Imprinting, Developmental Genetics Programme, The Babraham Institute, Cambridge CB2 4AT, United Kingdom2

Received 15 May 2003/ Returned for modification 24 July 2003/ Accepted 15 September 2003

Genomic imprinting at the Igf2/H19 locus originates from allele-specific DNA methylation, which modifies the affinity of some proteins for their target sequences. Here, we show that AT-rich DNA sequences located in the vicinity of previously characterized differentially methylated regions (DMRs) of the imprinted Igf2 gene are conserved between mouse and human. These sequences have all the characteristics of matrix attachment regions (MARs), which are known as versatile regulatory elements involved in chromatin structure and gene expression. Combining allele-specific nuclear matrix binding assays and real-time PCR quantification, we show that retention of two of these Igf2 MARs (MAR0 and MAR2) in the nuclear matrix fraction depends on the tissue and is specific to the paternal allele. Furthermore, on this allele, the Igf2 MAR2 is functionally linked to the neighboring DMR2 while, on the maternal allele, it is controlled by the imprinting-control region. Our work clearly demonstrates that genomic imprinting controls matrix attachment regions in the Igf2 gene.

* Corresponding author. Mailing address: IGMM, UMR5535 CNRS-UMII, IFR122, 1919, Route de Mende, 34293 Montpellier Cedex 5, France. Phone: 33 467 61 36 84. Fax: 33 467 04 02 31. E-mail: forne{at}igm.cnrs-mop.fr.

Molecular and Cellular Biology, December 2003, p. 8953-8959, Vol. 23, No. 24
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.24.8953-8959.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Berteaux, N., Aptel, N., Cathala, G., Genton, C., Coll, J., Daccache, A., Spruyt, N., Hondermarck, H., Dugimont, T., Curgy, J.-J., Forne, T., Adriaenssens, E. (2008). A Novel H19 Antisense RNA Overexpressed in Breast Cancer Contributes to Paternal IGF2 Expression. Mol. Cell. Biol. 28: 6731-6745 [Abstract] [Full Text]  
  • Braem, C., Recolin, B., Rancourt, R. C., Angiolini, C., Barthes, P., Branchu, P., Court, F., Cathala, G., Ferguson-Smith, A. C., Forne, T. (2008). Genomic Matrix Attachment Region and Chromosome Conformation Capture Quantitative Real Time PCR Assays Identify Novel Putative Regulatory Elements at the Imprinted Dlk1/Gtl2 Locus. J. Biol. Chem. 283: 18612-18620 [Abstract] [Full Text]  
  • Ling, J. Q., Li, T., Hu, J. F., Vu, T. H., Chen, H. L., Qiu, X. W., Cherry, A. M., Hoffman, A. R. (2006). CTCF mediates interchromosomal colocalization between Igf2/H19 and Wsb1/Nf1.. Science 312: 269-272 [Abstract] [Full Text]  
  • Thorvaldsen, J. L., Fedoriw, A. M., Nguyen, S., Bartolomei, M. S. (2006). Developmental Profile of H19 Differentially Methylated Domain (DMD) Deletion Alleles Reveals Multiple Roles of the DMD in Regulating Allelic Expression and DNA Methylation at the Imprinted H19/Igf2 Locus. Mol. Cell. Biol. 26: 1245-1258 [Abstract] [Full Text]  
  • Weidman, J. R., Murphy, S. K., Nolan, C. M., Dietrich, F. S., Jirtle, R. L. (2004). Phylogenetic Footprint Analysis of IGF2 in Extant Mammals. Genome Res 14: 1726-1732 [Abstract] [Full Text]  
  • Yusufzai, T. M., Felsenfeld, G. (2004). The 5'-HS4 chicken {beta}-globin insulator is a CTCF-dependent nuclear matrix-associated element. Proc. Natl. Acad. Sci. USA 101: 8620-8624 [Abstract] [Full Text]  
  • REIK, W., MURRELL, A., LEWIS, A., MITSUYA, K., UMLAUF, D., DEAN, W., HIGGINS, M., FEIL, R. (2004). Chromosome Loops, Insulators, and Histone Methylation: New Insights into Regulation of Imprinting in Clusters. Cold Spring Harb Symp Quant Biol 69: 29-38 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»