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Molecular and Cellular Biology, December 2003, p. 9283-9292, Vol. 23, No. 24
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.24.9283-9292.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Novel Methyltransferase for Modified Uridine Residues at the Wobble Position of tRNA

Hamid R. Kalhor and Steven Clarke*

Department of Chemistry and Biochemistry and Molecular Biology Institute, University of California, Los Angeles, California 90095

Received 6 March 2003/ Returned for modification 5 May 2003/ Accepted 5 September 2003

We have identified a novel tRNA methyltransferase in Saccharomyces cerevisiae that we designate Trm9. This enzyme, the product of theYML014w gene, catalyzes the esterification of modified uridine nucleotides, resulting in the formation of 5-methylcarbonylmethyluridine in tRNAArg3 and 5-methylcarbonylmethyl-2-thiouridine in tRNAGlu. In intact yeast cells, disruption of the TRM9 gene results in the complete loss of these modified wobble bases and increased sensitivity at 37°C to paromomycin, a translational inhibitor. These results suggest a role for this potentially reversible methyl esterification reaction when cells are under stress.

* Corresponding author. Mailing address: UCLA Molecular Biology Institute, 611 Charles E. Young Dr. East, Los Angeles, CA 90095-1570. Phone: (310) 825-8754. Fax: (310) 825-1968. E-mail: clarke{at}mbi.ucla.edu.

Molecular and Cellular Biology, December 2003, p. 9283-9292, Vol. 23, No. 24
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.24.9283-9292.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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