This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kimura, T.
Right arrow Articles by Nakano, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kimura, T.
Right arrow Articles by Nakano, T.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, February 2003, p. 1304-1315, Vol. 23, No. 4
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.4.1304-1315.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Mouse Germ Cell-Less as an Essential Component for Nuclear Integrity

Tohru Kimura,1 Chizuru Ito,2 Shoko Watanabe,1 Tohru Takahashi,3 Masahito Ikawa,4 Kentaro Yomogida,5 Yukiko Fujita,1 Megumi Ikeuchi,1 Noriko Asada,1 Kiyomi Matsumiya,3 Akihiko Okuyama,3 Masaru Okabe,4 Kiyotaka Toshimori,2 and Toru Nakano1*

Department of Molecular Cell Biology,1 Department of Laboratory Sciences for Animal Experimentation, Research Institute for Microbial Diseases, Osaka University, Suita-shi, Osaka 565-0871,5 Department of Anatomy and Reproductive Cell Biology, Miyazaki Medical College, Kiyotake, Miyazaki 889-1692,2 Department of Urology, Osaka University Medical School, Suita-shi, Osaka 565-0871,3 Genome Information Research Center, Osaka University, Suita-shi, Osaka 565-0871, Japan4

Received 19 September 2002/ Returned for modification 16 October 2002/ Accepted 18 November 2002

A mouse homologue of the Drosophila melanogaster germ cell-less (mgcl-1) gene is expressed ubiquitously, and its gene product is localized to the nuclear envelope based on its binding to LAP2ß (lamina-associated polypeptide 2ß). To elucidate the role of mgcl-1, we analyzed two mutant mouse lines that lacked mgcl-1 gene expression. Abnormal nuclear morphologies that were probably due to impaired nuclear envelope integrity were observed in the liver, exocrine pancreas, and testis. In particular, functional abnormalities were observed in testis in which the highest expression of mgcl-1 was detected. Fertility was significantly impaired in mgcl-1-null male mice, probably as a result of severe morphological abnormalities in the sperm. Electron microscopic observations showed insufficient chromatin condensation and abnormal acrosome structures in mgcl-1-null sperm. In addition, the expression patterns of transition proteins and protamines, both of which are essential for chromatin remodeling during spermatogenesis, were aberrant. Considering that the first abnormality during the process of spermatogenesis was abnormal nuclear envelope structure in spermatocytes, the mgcl-1 gene product appears to be essential for appropriate nuclear-lamina organization, which in turn is essential for normal sperm morphogenesis and chromatin remodeling.

* Corresponding author. Mailing address: Department of Molecular Cell Biology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan. Phone: 81-6-6879-8361. Fax: 81-6-6879-8362. E-mail: tnakano{at}biken.osaka-u.ac.jp.

Molecular and Cellular Biology, February 2003, p. 1304-1315, Vol. 23, No. 4
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.4.1304-1315.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nakamura, T., Inoue, K., Ogawa, S., Umehara, H., Ogonuki, N., Miki, H., Kimura, T., Ogura, A., Nakano, T. (2008). Effects of Akt signaling on nuclear reprogramming. GENES CELLS 13: 1269-1277 [Abstract] [Full Text]  
  • Murayama, E., Katoh, M., Kanebayashi, A., Kaneko, T., Shibata, Y., Inai, T., Iida, H. (2007). Germ cell-less like-2 protein is a new component of outer dense fibers in rat sperm flagella. Reproduction 134: 749-756 [Abstract] [Full Text]  
  • Umehara, H., Kimura, T., Ohtsuka, S., Nakamura, T., Kitajima, K., Ikawa, M., Okabe, M., Niwa, H., Nakano, T. (2007). Efficient Derivation of Embryonic Stem Cells by Inhibition of Glycogen Synthase Kinase-3. Stem Cells 25: 2705-2711 [Abstract] [Full Text]  
  • Nagamori, I., Yomogida, K., Ikawa, M., Okabe, M., Yabuta, N., Nojima, H. (2006). The testes-specific bZip type transcription factor Tisp40 plays a role in ER stress responses and chromatin packaging during spermiogenesis.. GENES CELLS 11: 1161-1171 [Abstract] [Full Text]  
  • Ellis, P. J.I., Clemente, E. J., Ball, P., Toure, A., Ferguson, L., Turner, J. M.A., Loveland, K. L., Affara, N. A., Burgoyne, P. S. (2005). Deletions on mouse Yq lead to upregulation of multiple X- and Y-linked transcripts in spermatids. Hum Mol Genet 14: 2705-2715 [Abstract] [Full Text]  
  • Somech, R., Shaklai, S., Geller, O., Amariglio, N., Simon, A. J., Rechavi, G., Gal-Yam, E. N. (2005). The nuclear-envelope protein and transcriptional repressor LAP2{beta} interacts with HDAC3 at the nuclear periphery, and induces histone H4 deacetylation. J. Cell Sci. 118: 4017-4025 [Abstract] [Full Text]  
  • Tanaka, H., Iguchi, N., Isotani, A., Kitamura, K., Toyama, Y., Matsuoka, Y., Onishi, M., Masai, K., Maekawa, M., Toshimori, K., Okabe, M., Nishimune, Y. (2005). HANP1/H1T2, a Novel Histone H1-Like Protein Involved in Nuclear Formation and Sperm Fertility. Mol. Cell. Biol. 25: 7107-7119 [Abstract] [Full Text]  
  • Juneja, S. C., van Deursen, J. M. (2005). A mouse model of familial oligoasthenoteratozoospermia. Hum Reprod 20: 881-893 [Abstract] [Full Text]  
  • Kuramochi-Miyagawa, S., Kimura, T., Ijiri, T. W., Isobe, T., Asada, N., Fujita, Y., Ikawa, M., Iwai, N., Okabe, M., Deng, W., Lin, H., Matsuda, Y., Nakano, T. (2004). Mili, a mammalian member of piwi family gene, is essential for spermatogenesis. Development 131: 839-849 [Abstract] [Full Text]  
  • Kleiman, S. E., Yogev, L., Gal-Yam, E. N., Hauser, R., Gamzu, R., Botchan, A., Paz, G., Yavetz, H., Maymon, B. B.-S., Schreiber, L., Barzilai, S., Amariglio, N., Rechavi, G., Simon, A. J. (2003). Reduced Human Germ Cell-Less (HGCL) Expression in Azoospermic Men With Severe Germinal Cell Impairment. J Androl 24: 670-675 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»