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Molecular and Cellular Biology, February 2003, p. 1403-1417, Vol. 23, No. 4
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.4.1403-1417.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Molecular Dissection of Mitotic Recombination in the Yeast Saccharomyces cerevisiae

Yael Aylon, Batia Liefshitz, Gili Bitan-Banin, and Martin Kupiec*

Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv 69978, Israel

Received 28 August 2002/ Returned for modification 10 October 2002/ Accepted 18 November 2002

Recombination plays a central role in the repair of broken chromosomes in all eukaryotes. We carried out a systematic study of mitotic recombination. Using several assays, we established the chronological sequence of events necessary to repair a single double-strand break. Once a chromosome is broken, yeast cells become immediately committed to recombinational repair. Recombination is completed within an hour and exhibits two kinetic gaps. By using this kinetic framework we also characterized the role played by several proteins in the recombinational process. In the absence of Rad52, the broken chromosome ends, both 5' and 3', are rapidly degraded. This is not due to the inability to recombine, since the 3' single-stranded DNA ends are stable in a strain lacking donor sequences. Rad57 is required for two consecutive strand exchange reactions. Surprisingly, we found that the Srs2 helicase also plays an early positive role in the recombination process.

* Corresponding author. Mailing address: Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv 69978, Israel. Phone: 972-3-640-9031. Fax: 972-3-640-9407. E-mail: martin{at}post.tau.ac.il.

Molecular and Cellular Biology, February 2003, p. 1403-1417, Vol. 23, No. 4
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.4.1403-1417.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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