This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nicolas, E.
Right arrow Articles by Trouche, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nicolas, E.
Right arrow Articles by Trouche, D.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, March 2003, p. 1614-1622, Vol. 23, No. 5
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.5.1614-1622.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Balance between Acetylation and Methylation of Histone H3 Lysine 9 on the E2F-Responsive Dihydrofolate Reductase Promoter

Estelle Nicolas, Christine Roumillac, and Didier Trouche*

Laboratoire de Biologie Moléculaire Eucaryote, UMR 5099 CNRS, IFR 109, Toulouse, France,

Received 31 October 2002/ Accepted 10 December 2002

Epigenetic marks that specify silent heterochromatic domains in eucaryotic genomes include methylation of histone H3 lysine 9. Strikingly, active loci in the vicinity of silent domains are sometimes characterized by acetylation of histone H3 lysine 9, suggesting that the balance between these two competitive modifications is important for the establishment of specific chromatin structures. Some euchromatic genes, targeted by the retinoblastoma protein Rb, are also believed to be regulated by histone H3 lysine 9 methylation. Here, we study the dihydrofolate reductase promoter, which is repressed in G0 and at the beginning of G1 by p107 or p130, two Rb-related proteins. We found that these two pocket proteins share with Rb the ability to associate with the histone methyl transferase SUV39H1. SUV39H1 can be recruited to the E2F transcription factor and functions as a transcriptional corepressor. With ChIP assays followed by real-time PCR, we showed that K9 of histone H3 evolves from a hypermethylated state in G0 to a hyperacetylated state at the G1/S transition. Taken together, these results indicate that the temporal regulation of euchromatic promoters may involve controlling the balance between methylation and acetylation of histone H3 lysine 9, a feature previously described for the spatial regulation of chromatin function.

* Corresponding author. Mailing address: LBME, UMR 5099 CNRS, 118 Route de Narbonne, 31062 Toulouse Cedex, France. Phone: 33 5 61 33 59 15. Fax: 33 5 61 33 58 86. E-mail: trouche{at}ibcg.biotoul.fr.

Molecular and Cellular Biology, March 2003, p. 1614-1622, Vol. 23, No. 5
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.5.1614-1622.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Cuevas-Bennett, C., Shenk, T. (2008). Dynamic Histone H3 Acetylation and Methylation at Human Cytomegalovirus Promoters during Replication in Fibroblasts. J. Virol. 82: 9525-9536 [Abstract] [Full Text]  
  • Vandromme, M., Chailleux, C., Escaffit, F., Trouche, D. (2008). Binding of the Retinoblastoma Protein Is Not the Determinant for Stable Repression of Some E2F-Regulated Promoters in Muscle Cells. Mol Cancer Res 6: 418-425 [Abstract] [Full Text]  
  • Gunawardena, R. W., Fox, S. R., Siddiqui, H., Knudsen, E. S. (2007). SWI/SNF Activity Is Required for the Repression of Deoxyribonucleotide Triphosphate Metabolic Enzymes via the Recruitment of mSin3B. J. Biol. Chem. 282: 20116-20123 [Abstract] [Full Text]  
  • Morris, S. A., Rao, B., Garcia, B. A., Hake, S. B., Diaz, R. L., Shabanowitz, J., Hunt, D. F., Allis, C. D., Lieb, J. D., Strahl, B. D. (2007). Identification of Histone H3 Lysine 36 Acetylation as a Highly Conserved Histone Modification. J. Biol. Chem. 282: 7632-7640 [Abstract] [Full Text]  
  • Garcia, B. A., Hake, S. B., Diaz, R. L., Kauer, M., Morris, S. A., Recht, J., Shabanowitz, J., Mishra, N., Strahl, B. D., Allis, C. D., Hunt, D. F. (2007). Organismal Differences in Post-translational Modifications in Histones H3 and H4. J. Biol. Chem. 282: 7641-7655 [Abstract] [Full Text]  
  • Chen, H., Yan, Y., Davidson, T. L., Shinkai, Y., Costa, M. (2006). Hypoxic Stress Induces Dimethylated Histone H3 Lysine 9 through Histone Methyltransferase G9a in Mammalian Cells.. Cancer Res. 66: 9009-9016 [Abstract] [Full Text]  
  • Bradley, S. P., Kaminski, D. A., Peters, A. H. F. M., Jenuwein, T., Stavnezer, J. (2006). The Histone Methyltransferase Suv39h1 Increases Class Switch Recombination Specifically to IgA. J. Immunol. 177: 1179-1188 [Abstract] [Full Text]  
  • Louie, M. C., Revenko, A. S., Zou, J. X., Yao, J., Chen, H.-W. (2006). Direct Control of Cell Cycle Gene Expression by Proto-Oncogene Product ACTR, and Its Autoregulation Underlies Its Transforming Activity. Mol. Cell. Biol. 26: 3810-3823 [Abstract] [Full Text]  
  • Fu, M., Rao, M., Bouras, T., Wang, C., Wu, K., Zhang, X., Li, Z., Yao, T.-P., Pestell, R. G. (2005). Cyclin D1 Inhibits Peroxisome Proliferator-activated Receptor {gamma}-mediated Adipogenesis through Histone Deacetylase Recruitment. J. Biol. Chem. 280: 16934-16941 [Abstract] [Full Text]  
  • Liu, D. X., Nath, N., Chellappan, S. P., Greene, L. A. (2005). Regulation of neuron survival and death by p130 and associated chromatin modifiers. Genes Dev. 19: 719-732 [Abstract] [Full Text]  
  • Fu, Q., McKnight, R. A., Yu, X., Wang, L., Callaway, C. W., Lane, R. H. (2004). Uteroplacental insufficiency induces site-specific changes in histone H3 covalent modifications and affects DNA-histone H3 positioning in day 0 IUGR rat liver. Physiol. Genomics 20: 108-116 [Abstract] [Full Text]  
  • Hulit, J., Wang, C., Li, Z., Albanese, C., Rao, M., Di Vizio, D., Shah, S., Byers, S. W., Mahmood, R., Augenlicht, L. H., Russell, R., Pestell, R. G. (2004). Cyclin D1 Genetic Heterozygosity Regulates Colonic Epithelial Cell Differentiation and Tumor Number in ApcMin Mice. Mol. Cell. Biol. 24: 7598-7611 [Abstract] [Full Text]  
  • Taubert, S., Gorrini, C., Frank, S. R., Parisi, T., Fuchs, M., Chan, H.-M., Livingston, D. M., Amati, B. (2004). E2F-Dependent Histone Acetylation and Recruitment of the Tip60 Acetyltransferase Complex to Chromatin in Late G1. Mol. Cell. Biol. 24: 4546-4556 [Abstract] [Full Text]  
  • Frolov, M. V., Dyson, N. J. (2004). Molecular mechanisms of E2F-dependent activation and pRB-mediated repression. J. Cell Sci. 117: 2173-2181 [Abstract] [Full Text]  
  • Sun, J., Brand, M., Zenke, Y., Tashiro, S., Groudine, M., Igarashi, K. (2004). Heme regulates the dynamic exchange of Bach1 and NF-E2-related factors in the Maf transcription factor network. Proc. Natl. Acad. Sci. USA 101: 1461-1466 [Abstract] [Full Text]  
  • Istomina, N. E., Shushanov, S. S., Springhetti, E. M., Karpov, V. L., Krasheninnikov, I. A., Stevens, K., Zaret, K. S., Singh, P. B., Grigoryev, S. A. (2003). Insulation of the Chicken {beta}-Globin Chromosomal Domain from a Chromatin-Condensing Protein, MENT. Mol. Cell. Biol. 23: 6455-6468 [Abstract] [Full Text]  
  • Chakrabarti, S. K., Francis, J., Ziesmann, S. M., Garmey, J. C., Mirmira, R. G. (2003). Covalent Histone Modifications Underlie the Developmental Regulation of Insulin Gene Transcription in Pancreatic {beta} Cells. J. Biol. Chem. 278: 23617-23623 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»