This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hobbs, J. A. R. Articles by Hogg, N. Search for Related Content PubMed PubMed Citation Articles by Hobbs, J. A. R. Articles by Hogg, N.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, April 2003, p. 2564-2576, Vol. 23, No. 7
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.7.2564-2576.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Myeloid Cell Function in MRP-14 (S100A9) Null Mice

Josie A. R. Hobbs, Richard May, Kiki Tanousis, Eileen McNeill, Margaret Mathies, Christoffer Gebhardt, Robert Henderson, Matthew J. Robinson,^¶ and Nancy Hogg^*

Leukocyte Adhesion Laboratory, Cancer Research UK, London Research Institute, Lincoln's Inn Fields Laboratories, London WC2A 3PX, United Kingdom,

Received 3 June 2002/ Returned for modification 31 July 2002/ Accepted 7 January 2003

Myeloid-related protein 14 (MRP-14) and its heterodimeric partner, MRP-8, are cytosolic calcium-binding proteins, highly expressed in neutrophils and monocytes. To understand the function of MRP-14, we performed targeted disruption of the MRP-14 gene in mice. MRP-14^-/- mice showed no obvious phenotype and were fertile. MRP-8 mRNA but not protein is present in the myeloid cells of these mice, suggesting that the stability of MRP-8 protein is dependent on MRP-14 expression. A compensatory increase in other proteins was not detected in cells lacking MRP-8 and MRP-14. Although the morphology of MRP-14^-/- myeloid cells was not altered, they were significantly less dense. When Ca²⁺ responses were investigated, there was no change in the maximal response to the chemokine MIP-2. At lower concentrations, however, there was reduced responsiveness in MRP-14^-/- compared with MRP-14^+/+ neutrophils. This alteration in the ability to flux Ca²⁺ did not impair the ability of the MRP-14^-/- neutrophils to respond chemotactically to MIP-2. In addition, the myeloid cell functions of phagocytosis, superoxide burst, and apoptosis were unaffected in MRP-14^-/- cells. In an in vivo model of peritonitis, MRP-14^-/- mice showed no difference from wild-type mice in induced inflammatory response. The data indicate that MRP-14 and MRP-8 are dispensable for many myeloid cell functions.

Present address: Cambridge Antibody Technology, Melbourn SG8 6JJ, United Kingdom.

Present address: W. M. Keck Science Center, The Claremont Colleges, Claremont, CA 91711.

Present address: Division of Signal Transduction and Growth Control, German Cancer Research Centre, 69120 Heidelberg, Germany.

^¶ Present address: Immune Cell Biology, National Institute of Medical Research, Mill Hill, London NW7 1AA, United Kingdom

This article has been cited by other articles:

• Hernandez-Novoa, B., Bishop, L., Logun, C., Munson, P. J., Elnekave, E., Rangel, Z. G., Barb, J., Danner, R. L., Kovacs, J. A. (2008). Immune responses to Pneumocystis murina are robust in healthy mice but largely absent in CD40 ligand-deficient mice. J. Leukoc. Biol. 84: 420-430 [Abstract] [Full Text]  
• Woodfin, A., Reichel, C. A., Khandoga, A., Corada, M., Voisin, M.-B., Scheiermann, C., Haskard, D. O., Dejana, E., Krombach, F., Nourshargh, S. (2007). JAM-A mediates neutrophil transmigration in a stimulus-specific manner in vivo: evidence for sequential roles for JAM-A and PECAM-1 in neutrophil transmigration. Blood 110: 1848-1856 [Abstract] [Full Text]  
• Stygar, D., Masironi, B., Eriksson, H., Sahlin, L. (2007). Studies on estrogen receptor (ER) {alpha} and {beta} responses on gene regulation in peripheral blood leukocytes in vivo using selective ER agonists. J Endocrinol 194: 101-119 [Abstract] [Full Text]  
• Sasmono, R. T., Ehrnsperger, A., Cronau, S. L., Ravasi, T., Kandane, R., Hickey, M. J., Cook, A. D., Himes, S. R., Hamilton, J. A., Hume, D. A. (2007). Mouse neutrophilic granulocytes express mRNA encoding the macrophage colony-stimulating factor receptor (CSF-1R) as well as many other macrophage-specific transcripts and can transdifferentiate into macrophages in vitro in response to CSF-1. J. Leukoc. Biol. 82: 111-123 [Abstract] [Full Text]  
• Sroussi, H. Y., Berline, J., Palefsky, J. M. (2007). Oxidation of methionine 63 and 83 regulates the effect of S100A9 on the migration of neutrophils in vitro. J. Leukoc. Biol. 81: 818-824 [Abstract] [Full Text]  
• Lominadze, G., Rane, M. J., Merchant, M., Cai, J., Ward, R. A., McLeish, K. R. (2005). Myeloid-Related Protein-14 Is a p38 MAPK Substrate in Human Neutrophils. J. Immunol. 174: 7257-7267 [Abstract] [Full Text]  
• Hsu, K., Passey, R. J., Endoh, Y., Rahimi, F., Youssef, P., Yen, T., Geczy, C. L. (2005). Regulation of S100A8 by Glucocorticoids. J. Immunol. 174: 2318-2326 [Abstract] [Full Text]  
• Vogl, T., Ludwig, S., Goebeler, M., Strey, A., Thorey, I. S., Reichelt, R., Foell, D., Gerke, V., Manitz, M. P., Nacken, W., Werner, S., Sorg, C., Roth, J. (2004). MRP8 and MRP14 control microtubule reorganization during transendothelial migration of phagocytes. Blood 104: 4260-4268 [Abstract] [Full Text]