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Molecular and Cellular Biology, April 2003, p. 2614-2622, Vol. 23, No. 7
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.7.2614-2622.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Disruption of Mouse CD46 Causes an Accelerated Spontaneous Acrosome Reaction in Sperm

Naokazu Inoue,1 Masahito Ikawa,2 Tomoko Nakanishi,2 Misako Matsumoto,1 Midori Nomura,1 Tsukasa Seya,1 and Masaru Okabe2*

Department of Immunology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Higashinari-ku, Osaka 537-8511,1 Genome Information Research Center, Osaka University, Suita, Osaka 565-0871, Japan2

Received 3 September 2002/ Returned for modification 1 November 2002/ Accepted 19 December 2002

Human membrane cofactor protein (MCP, CD46) is a ubiquitously expressed protein known to protect cells from complement attack. Interestingly, when we examined the expression of mouse CD46, which we recently cloned, the message was found only in testis and the protein was found on the inner acrosomal membrane of sperm. In order to elucidate the function of CD46, we produced mice carrying a null mutation in the CD46 gene by using homologous recombination. Despite the absence of CD46, the mice were healthy and both sexes were fertile. However, to our surprise, the fertilizing ability of males appeared to be facilitated by disruption of the CD46 gene, as the average number of pups born from CD46-/- males was significantly greater than that of wild-type males. It was also revealed that the incidence of the spontaneous acrosome reaction doubled in CD46-/- sperm compared to that in wild-type sperm. It was assumed that this increase caused the heightened fertilizing ability found in CD46-/- sperm. These data suggest that CD46 may have some role in regulating sperm acrosome reaction.

* Corresponding author. Mailing address: Genome Information Research Center, Osaka University, Yamadaoka 3-1, Suita, Osaka 565-087, Japan. Phone: (81)-6-6879-8374. Fax: (81)-6-6879-8376. E-mail: okabe{at}gen-info.osaka-u.ac.jp.

Molecular and Cellular Biology, April 2003, p. 2614-2622, Vol. 23, No. 7
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.7.2614-2622.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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