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Molecular and Cellular Biology, April 2003, p. 2623-2632, Vol. 23, No. 8
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.8.2623-2632.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

A Yeast Homologue of Hsp70, Ssa1p, Regulates Turnover of the MFA2 Transcript through Its AU-Rich 3' Untranslated Region

Radharani Duttagupta,1 Shobha Vasudevan,1 Carol J. Wilusz,1* and Stuart W. Peltz1,2

Department of Molecular Genetics, Microbiology and Immunology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School,1 The Cancer Institute of New Jersey, Piscataway, New Jersey 088542

Received 8 November 2002/ Accepted 16 January 2003

Many eukaryotic mRNAs exhibit regulated decay in response to cellular signals. AU-rich elements (AREs) identified in the 3' untranslated region (3'-UTR) of several such mRNAs play a critical role in controlling the half-lives of these transcripts. The yeast ARE-containing mRNA, MFA2, has been studied extensively and is degraded by a deadenylation-dependent mechanism. However, the trans-acting factors that promote the rapid decay of MFA2 have not been identified. Our results suggest that the chaperone protein Hsp70, encoded by the SSA family of genes, is involved in modulating MFA2 mRNA decay. MFA2 is specifically stabilized in a strain bearing a temperature-sensitive mutation in the SSA1 gene. Furthermore, an AU-rich region within the 3'-UTR of the message is both necessary and sufficient to confer this regulation. Stabilization occurs as a result of slower deadenylation in the ssa1ts strain, suggesting that Hsp70 is required for activation of the turnover pathway.

* Corresponding author. Mailing address: Department of Molecular Genetics, Microbiology and Immunology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, 675 Hoes Ln., Piscataway, NJ 08854. Phone: (732) 235-4107. Fax: (732) 235-5223. E-mail: carol.wilusz{at}umdnj.edu.

Molecular and Cellular Biology, April 2003, p. 2623-2632, Vol. 23, No. 8
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.8.2623-2632.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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