This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schwerk, C.
Right arrow Articles by Reinberg, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schwerk, C.
Right arrow Articles by Reinberg, D.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, April 2003, p. 2981-2990, Vol. 23, No. 8
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.8.2981-2990.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

ASAP, a Novel Protein Complex Involved in RNA Processing and Apoptosis

Christian Schwerk,1,2 Jayendra Prasad,3 Kurt Degenhardt,1,4 Hediye Erdjument-Bromage,5 Eileen White,1,4 Paul Tempst,5 Vincent J. Kidd,6 James L. Manley,3 Jill M. Lahti,6 and Danny Reinberg1,2*

Howard Hughes Medical Institute,1 and Division of Nucleic Acids Enzymology, Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School,2 Center for Advanced Biotechnology and Medicine, Rutgers University, Piscataway, New Jersey 08854,4 Department of Biological Sciences, Columbia University, New York, New York 10027,3 Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021,5 Department of Genetics and Tumor Cell Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 381056

Received 11 November 2002/ Returned for modification 30 December 2002/ Accepted 23 January 2003

Different isoforms of a protein complex termed the apoptosis- and splicing-associated protein (ASAP) were isolated from HeLa cell extract. ASAP complexes are composed of the polypeptides SAP18 and RNPS1 and different isoforms of the Acinus protein. While Acinus had previously been implicated in apoptosis and was recently identified as a component of the spliceosome, RNPS1 has been described as a general activator of RNA processing. Addition of ASAP isoforms to in vitro splicing reactions inhibits RNA processing mediated by ASF/SF2, by SC35, or by RNPS1. Additionally, microinjection of ASAP complexes into mammalian cells resulted in acceleration of cell death. Importantly, after induction of apoptosis the ASAP complex disassembles. Taken together, our results suggest an important role for the ASAP complexes in linking RNA processing and apoptosis.

* Corresponding author. Mailing address: Howard Hughes Medical Institute, Division of Nucleic Acids Enzymology, Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, NJ 08854. Phone: (732) 235-4195. Fax: (732) 235-5294. E-mail: reinbedf{at}UMDNJ.edu.

Molecular and Cellular Biology, April 2003, p. 2981-2990, Vol. 23, No. 8
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.8.2981-2990.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Viiri, K. M., Janis, J., Siggers, T., Heinonen, T. Y. K., Valjakka, J., Bulyk, M. L., Maki, M., Lohi, O. (2009). DNA-Binding and -Bending Activities of SAP30L and SAP30 Are Mediated by a Zinc-Dependent Module and Monophosphoinositides. Mol. Cell. Biol. 29: 342-356 [Abstract] [Full Text]  
  • Jang, S.-W., Yang, S.-j., Ehlen, A., Dong, S., Khoury, H., Chen, J., Persson, J. L., Ye, K. (2008). Serine/Arginine Protein-Specific Kinase 2 Promotes Leukemia Cell Proliferation by Phosphorylating Acinus and Regulating Cyclin A1. Cancer Res. 68: 4559-4570 [Abstract] [Full Text]  
  • Vucetic, Z., Zhang, Z., Zhao, J., Wang, F., Soprano, K. J., Soprano, D. R. (2008). Acinus-S' Represses Retinoic Acid Receptor (RAR)-Regulated Gene Expression through Interaction with the B Domains of RARs. Mol. Cell. Biol. 28: 2549-2558 [Abstract] [Full Text]  
  • Li, X., Niu, T., Manley, J. L. (2007). The RNA binding protein RNPS1 alleviates ASF/SF2 depletion-induced genomic instability. RNA 13: 2108-2115 [Abstract] [Full Text]  
  • Ying, S., Fischer, S. F., Pettengill, M., Conte, D., Paschen, S. A., Ojcius, D. M., Hacker, G. (2006). Characterization of Host Cell Death Induced by Chlamydia trachomatis. Infect. Immun. 74: 6057-6066 [Abstract] [Full Text]  
  • Degenhardt, K., White, E. (2006). A mouse model system to genetically dissect the molecular mechanisms regulating tumorigenesis.. Clin. Cancer Res. 12: 5296-5304 [Abstract] [Full Text]  
  • Joselin, A. P., Schulze-Osthoff, K., Schwerk, C. (2006). Loss of Acinus Inhibits Oligonucleosomal DNA Fragmentation but Not Chromatin Condensation during Apoptosis. J. Biol. Chem. 281: 12475-12484 [Abstract] [Full Text]  
  • TANGE, T. O., SHIBUYA, T., JURICA, M. S., MOORE, M. J. (2005). Biochemical analysis of the EJC reveals two new factors and a stable tetrameric protein core. RNA 11: 1869-1883 [Abstract] [Full Text]  
  • Canudas, S., Perez, S., Fanti, L., Pimpinelli, S., Singh, N., Hanes, S. D., Azorin, F., Espinas, M. L. (2005). dSAP18 and dHDAC1 contribute to the functional regulation of the Drosophila Fab-7 element. Nucleic Acids Res 33: 4857-4864 [Abstract] [Full Text]  
  • Trembley, J. H., Tatsumi, S., Sakashita, E., Loyer, P., Slaughter, C. A., Suzuki, H., Endo, H., Kidd, V. J., Mayeda, A. (2005). Activation of Pre-mRNA Splicing by Human RNPS1 Is Regulated by CK2 Phosphorylation. Mol. Cell. Biol. 25: 1446-1457 [Abstract] [Full Text]  
  • Saitoh, N., Spahr, C. S., Patterson, S. D., Bubulya, P., Neuwald, A. F., Spector, D. L. (2004). Proteomic Analysis of Interchromatin Granule Clusters. Mol. Biol. Cell 15: 3876-3890 [Abstract] [Full Text]  
  • Vermeulen, M., Carrozza, M. J., Lasonder, E., Workman, J. L., Logie, C., Stunnenberg, H. G. (2004). In Vitro Targeting Reveals Intrinsic Histone Tail Specificity of the Sin3/Histone Deacetylase and N-CoR/SMRT Corepressor Complexes. Mol. Cell. Biol. 24: 2364-2372 [Abstract] [Full Text]  
  • Sakashita, E., Tatsumi, S., Werner, D., Endo, H., Mayeda, A. (2004). Human RNPS1 and Its Associated Factors: a Versatile Alternative Pre-mRNA Splicing Regulator In Vivo. Mol. Cell. Biol. 24: 1174-1187 [Abstract] [Full Text]  
  • Li, C., Lin, R.-I., Lai, M.-C., Ouyang, P., Tarn, W.-Y. (2003). Nuclear Pnn/DRS Protein Binds to Spliced mRNPs and Participates in mRNA Processing and Export via Interaction with RNPS1. Mol. Cell. Biol. 23: 7363-7376 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»