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Molecular and Cellular Biology, May 2003, p. 3116-3125, Vol. 23, No. 9
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.9.3116-3125.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

The Tap42-Protein Phosphatase Type 2A Catalytic Subunit Complex Is Required for Cell Cycle-Dependent Distribution of Actin in Yeast

Huamin Wang and Yu Jiang*

Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213

Received 31 October 2002/ Returned for modification 11 December 2002/ Accepted 6 February 2003

In Saccharomyces cerevisiae, the Tor proteins mediate a wide spectrum of growth-related cellular processes in response to nutrients. The pleiotropic role of the Tor proteins is mediated, at least in part, by type 2A protein phosphatases (PP2A) and 2A-like protein phosphatases. Tor-mediated signaling activity promotes the interaction of phosphatase-interacting protein Tap42 with PP2A and 2A-like protein phosphatases. The distinct complexes formed between Tap42 and different phosphatases mediate various cellular events and modulate phosphorylation levels of many downstream factors in the Tor pathway in a Tor-dependent and rapamycin-sensitive manner. In this study, we demonstrate that the interaction between Tap42 and the catalytic subunits of PP2A (PP2Ac) is required for cell cycle-dependent distribution of actin. We show that mutations in PP2Ac and Tap42 that perturb the interaction cause random distribution of actin during the cell cycle and that overexpression of the Rho2 GTPase suppresses the actin defects associated with the mutants. Our findings suggest that the Tap42-PP2Ac complex regulates the actin cytoskeleton via a Rho GTPase-dependent mechanism. In addition, we provide evidence that PP2A activity plays a negative role in controlling the actin cytoskeleton and, possibly, in regulation of the G2/M transition of the cell cycle.

* Corresponding author. Mailing address: Department of Pharmacology, University of Pittsburgh School of Medicine, E1357 Biological Science Tower, 200 Lothrop St., Pittsburgh, PA 15213. Phone: (412) 648-3390. Fax: (412) 648-1945. E-mail: jiang{at}server.pharm.pitt.edu.

Molecular and Cellular Biology, May 2003, p. 3116-3125, Vol. 23, No. 9
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.9.3116-3125.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kong, M., Bui, T. V., Ditsworth, D., Gruber, J. J., Goncharov, D., Krymskaya, V. P., Lindsten, T., Thompson, C. B. (2007). The PP2A-associated Protein {alpha}4 Plays a Critical Role in the Regulation of Cell Spreading and Migration. J. Biol. Chem. 282: 29712-29720 [Abstract] [Full Text]  
  • Aronova, S., Wedaman, K., Anderson, S., Yates, J. III, Powers, T. (2007). Probing the Membrane Environment of the TOR Kinases Reveals Functional Interactions between TORC1, Actin, and Membrane Trafficking in Saccharomyces cerevisiae. Mol. Biol. Cell 18: 2779-2794 [Abstract] [Full Text]  
  • Jiang, Y. (2006). Regulation of the Cell Cycle by Protein Phosphatase 2A in Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 70: 440-449 [Abstract] [Full Text]  
  • Giannattasio, S., Liu, Z., Thornton, J., Butow, R. A. (2005). Retrograde Response to Mitochondrial Dysfunction Is Separable from TOR1/2 Regulation of Retrograde Gene Expression. J. Biol. Chem. 280: 42528-42535 [Abstract] [Full Text]  
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  • Wang, H., Wang, X., Jiang, Y. (2003). Interaction with Tap42 Is Required for the Essential Function of Sit4 and Type 2A Phosphatases. Mol. Biol. Cell 14: 4342-4351 [Abstract] [Full Text]  


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