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Molecular and Cellular Biology, May 2003, p. 3274-3286, Vol. 23, No. 9
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.9.3274-3286.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Transcription Factor IIB (TFIIB)-Related Protein (pBrp), a Plant-Specific Member of the TFIIB-Related Protein Family

Thierry Lagrange,1* Mohamed-Ali Hakimi,1,{dagger} Dominique Pontier,1,2 Florence Courtois,1 Jean Pierre Alcaraz,1 Didier Grunwald,3 Eric Lam,2 and Silva Lerbs-Mache1

Laboratoire de Génétique Moléculaire des Plantes, UMR5575, 38041 Grenoble Cedex 9,1 Laboratoire Canaux Ioniques et Signalisation, INSERM E9931, DBMS, CEA, 38054 Grenoble Cedex 9, France,3 Biotech Center, Rutgers University, New Brunswick, New Jersey 089032

Received 25 November 2002/ Accepted 4 February 2003

Although it is now well documented that metazoans have evolved general transcription factor (GTF) variants to regulate their complex patterns of gene expression, there is so far no information regarding the existence of specific GTFs in plants. Here we report the characterization of a ubiquitously expressed gene that encodes a bona fide novel transcription factor IIB (TFIIB)-related protein in Arabidopsis thaliana. We have shown that this protein is the founding member of a plant-specific TFIIB-related protein family named pBrp (for plant-specific TFIIB-related protein). Surprisingly, in contrast to common GTFs that are localized in the nucleus, the bulk of pBrp proteins are bound to the cytoplasmic face of the plastid envelope, suggesting an organelle-specific function for this novel class of TFIIB-related protein. We show that pBrp proteins harbor conditional proteolytic signals that can target these proteins for rapid turnover by the proteasome-mediated protein degradation pathway. Interestingly, under conditions of proteasome inhibition, pBrp proteins accumulate in the nucleus. Together, our results suggest a possible involvement of these proteins in an intracellular signaling pathway between plastids and the nucleus. Our data provide the first evidence for an organelle-related evolution of the eukaryotic general transcription machinery.

* Corresponding author. Present address: Laboratoire Génome et Développement des Plantes, UMR 5096, Université de Perpignan et Centre National de la Recherche Scientifique, 52 Ave. de Villenueve, F-66860 Perpignan Cedex, France. Phone: 33 468 66 21 17. Fax: 33 468 66 84 99. E-mail: lagrange{at}univ-perp.fr.

{dagger} Present address: Laboratoire Adaptation et Pathogénie des Micro-organismes, FRE 2383, Centre National de la Recherche Scientifique, 38041, Grenoble Cedex 9, France.

Molecular and Cellular Biology, May 2003, p. 3274-3286, Vol. 23, No. 9
0022-538X/03/$08.00+0     DOI: 10.1128/MCB.23.9.3274-3286.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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