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Molecular and Cellular Biology, January 2004, p. 135-143, Vol. 24, No. 1
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.1.135-143.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Spt10-Dependent Transcriptional Activation in Saccharomyces cerevisiae Requires both the Spt10 Acetyltransferase Domain and Spt21

David Hess,1 Bingsheng Liu,2 Nadia R. Roan,1 Rolf Sternglanz,2 and Fred Winston1*

Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115,1 Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, New York 117942

Received 9 July 2003/ Returned for modification 11 September 2003/ Accepted 2 October 2003

Histone levels are a key factor in several nuclear processes, including transcription and chromosome segregation. Previous studies have demonstrated that Spt10 and Spt21 are required for the normal transcription of a subset of the histone genes in Saccharomyces cerevisiae, and sequence analysis has suggested that Spt10 is an acetyltransferase. We have now characterized several aspects of transcriptional activation of histone genes by Spt10 in vivo. Our results show that activation by Spt10 is dependent on its acetyltransferase domain. At HTA2-HTB2, the histone locus whose transcription is most strongly dependent on Spt10, Spt10 is physically recruited to the promoter in an Spt21-dependent and a cell cycle-dependent manner. Furthermore, Spt10 and Spt21 directly interact. These results, taken together with the identification of spt10 mutations that suppress an spt21{Delta} mutation, suggest a model for transcriptional activation by Spt10 and Spt21.

* Corresponding author. Mailing address: Department of Genetics, Harvard Medical School, 77 Ave. Louis Pasteur, Boston, MA 02115. Phone: (617) 432-7768. Fax: (617) 432-6506. E-mail: winston{at}genetics.med.harvard.edu.

Molecular and Cellular Biology, January 2004, p. 135-143, Vol. 24, No. 1
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.1.135-143.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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