This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miyagi, S.
Right arrow Articles by Okuda, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miyagi, S.
Right arrow Articles by Okuda, A.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, May 2004, p. 4207-4220, Vol. 24, No. 10
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.10.4207-4220.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

The Sox-2 Regulatory Regions Display Their Activities in Two Distinct Types of Multipotent Stem Cells

Satoru Miyagi,1,2 Tetsuichiro Saito,3 Ken-ichi Mizutani,3 Norihisa Masuyama,4 Yukiko Gotoh,4 Atsushi Iwama,5 Hiromitsu Nakauchi,5 Shinji Masui,6 Hitoshi Niwa,6 Masazumi Nishimoto,1 Masami Muramatsu,1 and Akihiko Okuda1*

Division of Developmental Biology, Research Center for Genomic Medicine, Saitama Medical School, Saitama 350-1241,1 REDS Group, Saitama Small Enterprise Promotion Corporation, Skip City, Kawaguchi, Saitama 333-0844,2 Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507,3 Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo 113-0032,4 Laboratory of Stem Cell Therapy, Center for Experimental Medicine, Institute of Medical Science, University of Tokyo, Tokyo 108-8639,5 Laboratory for Cell Pluripotency Studies, RIKEN, Center for Developmental Biology, Chuo-ku, Kobe 650-0047, Japan6

Received 4 November 2003/ Returned for modification 8 January 2004/ Accepted 18 February 2004

The Sox-2 gene is expressed in embryonic stem (ES) cells and neural stem cells. Two transcription enhancer regions, Sox-2 regulatory region 1 (SRR1) and SRR2, were described previously based on their activities in ES cells. Here, we demonstrate that these regulatory regions also exert their activities in neural stem cells. Moreover, our data reveal that, as in ES cells, both SRR1 and SRR2 show their activities rather specifically in multipotent neural stem or progenitor cells but cease to function in differentiated cells, such as postmitotic neurons. Systematic deletion and mutation analyses showed that the same or at least overlapping DNA elements of SRR2 are involved in its activity in both ES and neural stem or progenitor cells. Thus, SRR2 is the first example of an enhancer in which a single regulatory core sequence is involved in multipotent-state-specific expression in two different stem cells, i.e., ES and neural stem cells.

* Corresponding author. Mailing address: Division of Developmental Biology, Research Center for Genomic Medicine, Saitama Medical School, 1397-1 Yamane Hidaka, Saitama 350-1241, Japan. Phone: 81-429-85-7268. Fax: 81-429-85-7264. E-mail: akiokuda{at}saitama-med.ac.jp.

Molecular and Cellular Biology, May 2004, p. 4207-4220, Vol. 24, No. 10
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.10.4207-4220.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Amaral, P. P., Neyt, C., Wilkins, S. J., Askarian-Amiri, M. E., Sunkin, S. M., Perkins, A. C., Mattick, J. S. (2009). Complex architecture and regulated expression of the Sox2ot locus during vertebrate development. RNA 15: 2013-2027 [Abstract] [Full Text]  
  • Alatzoglou, K. S, Kelberman, D., Dattani, M. T (2009). The role of SOX proteins in normal pituitary development. J Endocrinol 200: 245-258 [Abstract] [Full Text]  
  • Yaragatti, M., Basilico, C., Dailey, L. (2008). Identification of active transcriptional regulatory modules by the functional assay of DNA from nucleosome-free regions. Genome Res 18: 930-938 [Abstract] [Full Text]  
  • Sakaguchi, T., Nishimoto, M., Miyagi, S., Iwama, A., Morita, Y., Iwamori, N., Nakauchi, H., Kiyonari, H., Muramatsu, M., Okuda, A. (2006). Putative "Stemness" Gene Jam-B Is Not Required for Maintenance of Stem Cell State in Embryonic, Neural, or Hematopoietic Stem Cells.. Mol. Cell. Biol. 26: 6557-6570 [Abstract] [Full Text]  
  • Miyagi, S., Nishimoto, M., Saito, T., Ninomiya, M., Sawamoto, K., Okano, H., Muramatsu, M., Oguro, H., Iwama, A., Okuda, A. (2006). The Sox2 Regulatory Region 2 Functions as a Neural Stem Cell-specific Enhancer in the Telencephalon. J. Biol. Chem. 281: 13374-13381 [Abstract] [Full Text]  
  • Nishimoto, M., Miyagi, S., Yamagishi, T., Sakaguchi, T., Niwa, H., Muramatsu, M., Okuda, A. (2005). Oct-3/4 Maintains the Proliferative Embryonic Stem Cell State via Specific Binding to a Variant Octamer Sequence in the Regulatory Region of the UTF1 Locus. Mol. Cell. Biol. 25: 5084-5094 [Abstract] [Full Text]  
  • Shirai, T., Miyagi, S., Horiuchi, D., Okuda-Katayanagi, T., Nishimoto, M., Muramatsu, M., Sakamoto, Y., Nagata, M., Hagiwara, K., Okuda, A. (2005). Identification of an Enhancer That Controls Up-regulation of Fibronectin during Differentiation of Embryonic Stem Cells into Extraembryonic Endoderm. J. Biol. Chem. 280: 7244-7252 [Abstract] [Full Text]  
  • Seo, S., Richardson, G. A., Kroll, K. L. (2005). The SWI/SNF chromatin remodeling protein Brg1 is required for vertebrate neurogenesis and mediates transactivation of Ngn and NeuroD. Development 132: 105-115 [Abstract] [Full Text]  
  • Catena, R., Tiveron, C., Ronchi, A., Porta, S., Ferri, A., Tatangelo, L., Cavallaro, M., Favaro, R., Ottolenghi, S., Reinbold, R., Scholer, H., Nicolis, S. K. (2004). Conserved POU Binding DNA Sites in the Sox2 Upstream Enhancer Regulate Gene Expression in Embryonic and Neural Stem Cells. J. Biol. Chem. 279: 41846-41857 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»