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Molecular and Cellular Biology, May 2004, p. 4221-4228, Vol. 24, No. 10
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.10.4221-4228.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Normal Embryonic and Germ Cell Development in Mice Lacking {alpha}1,3-Fucosyltransferase IX (Fut9) Which Show Disappearance of Stage-Specific Embryonic Antigen 1

Takashi Kudo,1,2,3,{dagger} Mika Kaneko,3,4 Hiroko Iwasaki,1,3,5 Akira Togayachi,1,2,3 Shoko Nishihara,3 Kuniya Abe,6 and Hisashi Narimatsu1,3*

Glycogene Function Team, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology, Open Space Laboratory, Tsukuba, Ibaraki 305-8568,1 New Energy and Industrial Technology Development Organization (NEDO), Toshima-ku, Tokyo 170-6028,2 Division of Cell Biology, Institute of Life Science, Soka University, Hachioji, Tokyo 192-8577,3 Department of Forensic Medicine, Yamagata University School of Medicine, Yamagata 990-9585,4 Amersham Biosciences KK, Shinjuku-ku, Tokyo 169-0073,5 Technology and Development Team for Mammalian Cellular Dynamics, RIKEN Tsukuba Institute, Bioresource Center, Tsukuba, Ibaraki 305-0074, Japan6

Received 13 August 2003/ Returned for modification 24 October 2003/ Accepted 16 February 2004

Stage-specific embryonic antigen 1 (SSEA-1), an antigenic epitope defined as a Lewis x carbohydrate structure, is expressed during the 8-cell to blastocyst stages in mouse embryos and in primordial germ cells, undifferentiated embryonic stem cells, and embryonic carcinoma cells. For many years, SSEA-1 has been implicated in the development of mouse embryos as a functional carbohydrate epitope in cell-to-cell interaction during morula compaction. In a previous study, {alpha}1,3-fucosyltransferase IX (Fut9) exhibited very strong activity for the synthesis of Lewis x compared to other {alpha}1,3-fucosyltransferases in an in vitro substrate specificity assay. Fut4 and Fut9 transcripts were expressed in mouse embryos. The Fut9 transcript was detected in embryonic-day-13.5 gonads containing primordial germ cells, but the Fut4 transcript was not. In order to identify the role of SSEA-1 and determine the key enzyme for SSEA-1 synthesis in vivo, we have generated Fut9-deficient (Fut9–/–) mice. Fut9–/– mice develop normally, with no gross phenotypic abnormalities, and are fertile. Immunohistochemical analysis revealed an absence of SSEA-1 expression in early embryos and primordial germ cells of Fut9–/– mice. Therefore, we conclude that expression of the SSEA-1 epitope in the developing mouse embryo is not essential for embryogenesis in vivo.

* Corresponding author. Mailing address: Glycogene Function Team, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Central-2, Open Space Laboratory, 1-1-1 Umezono, Tsukuba, Ibaraki 305-8568, Japan. Phone: 81-29-861-3200. Fax: 81-29-861-3201. E-mail: h.narimatsu{at}aist.go.jp.

{dagger} Present address: Departments of Anatomy and Embryology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan.

Molecular and Cellular Biology, May 2004, p. 4221-4228, Vol. 24, No. 10
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.10.4221-4228.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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