This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Soustelle, C.
Right arrow Articles by Heude, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Soustelle, C.
Right arrow Articles by Heude, M.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, June 2004, p. 5130-5143, Vol. 24, No. 12
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.12.5130-5143.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

A New Saccharomyces cerevisiae Strain with a Mutant Smt3-Deconjugating Ulp1 Protein Is Affected in DNA Replication and Requires Srs2 and Homologous Recombination for Its Viability

Christine Soustelle ,1,{dagger},{ddagger} Laurence Vernis,2,{dagger} Karine Fréon,2 Anne Reynaud-Angelin,2 Roland Chanet,2 Francis Fabre,1 and Martine Heude2*

Commissariat à l'Energie Atomique, UMR 217 CNRS-CEA, DSV/DRR, 92265 Fontenay-aux-Roses,1 Institut Curie, UMR 2027/CNRS-Institut Curie, Génotoxicologie et Cycle Cellulaire, Centre Universitaire, 91405 Orsay Cedex, France2

Received 24 December 2003/ Returned for modification 25 February 2004/ Accepted 22 March 2004

The Saccharomyces cerevisiae Srs2 protein is involved in DNA repair and recombination. In order to gain better insight into the roles of Srs2, we performed a screen to identify mutations that are synthetically lethal with an srs2 deletion. One of them is a mutated allele of the ULP1 gene that encodes a protease specifically cleaving Smt3-protein conjugates. This allele, ulp1-I615N, is responsible for an accumulation of Smt3-conjugated proteins. The mutant is unable to grow at 37°C. At permissive temperatures, it still shows severe growth defects together with a strong hyperrecombination phenotype and is impaired in meiosis. Genetic interactions between ulp1 and mutations that affect different repair pathways indicated that the RAD51-dependent homologous recombination mechanism, but not excision resynthesis, translesion synthesis, or nonhomologous end-joining processes, is required for the viability of the mutant. Thus, both Srs2, believed to negatively control homologous recombination, and the process of recombination per se are essential for the viability of the ulp1 mutant. Upon replication, mutant cells accumulate single-stranded DNA interruptions. These structures are believed to generate different recombination intermediates. Some of them are fixed by recombination, and others require Srs2 to be reversed and fixed by an alternate pathway.


* Corresponding author. Mailing address: Institut Curie, UMR 2027/CNRS-Institut Curie, Génotoxicologie et Cycle Cellulaire, BÂtiment 110, Centre Universitaire, 91405 Orsay Cedex, France. Phone: (33) 1 69 86 30 56. Fax: (33) 1 69 86 94 29. E-mail: martine.heude{at}curie.u-psud.fr.

{dagger} C.S. and L.V. contributed equally to this work.

{ddagger} Present address: CNRS UPR 2167, Centre de Génétique Moléculaire, Equipe LIPM, 91198 Gif-sur-Yvette, France.


Molecular and Cellular Biology, June 2004, p. 5130-5143, Vol. 24, No. 12
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.12.5130-5143.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.




This article has been cited by other articles:

  • Nagai, S., Dubrana, K., Tsai-Pflugfelder, M., Davidson, M. B., Roberts, T. M., Brown, G. W., Varela, E., Hediger, F., Gasser, S. M., Krogan, N. J. (2008). Functional Targeting of DNA Damage to a Nuclear Pore-Associated SUMO-Dependent Ubiquitin Ligase. Science 322: 597-602 [Abstract] [Full Text]  
  • Mullen, J. R., Brill, S. J. (2008). Activation of the Slx5-Slx8 Ubiquitin Ligase by Poly-small Ubiquitin-like Modifier Conjugates. J. Biol. Chem. 283: 19912-19921 [Abstract] [Full Text]  
  • Xie, Y., Kerscher, O., Kroetz, M. B., McConchie, H. F., Sung, P., Hochstrasser, M. (2007). The Yeast Hex3{middle dot}Slx8 Heterodimer Is a Ubiquitin Ligase Stimulated by Substrate Sumoylation. J. Biol. Chem. 282: 34176-34184 [Abstract] [Full Text]  
  • Chen, X. L., Silver, H. R., Xiong, L., Belichenko, I., Adegite, C., Johnson, E. S. (2007). Topoisomerase I-Dependent Viability Loss in Saccharomyces cerevisiae Mutants Defective in Both SUMO Conjugation and DNA Repair. Genetics 177: 17-30 [Abstract] [Full Text]  
  • Palancade, B., Liu, X., Garcia-Rubio, M., Aguilera, A., Zhao, X., Doye, V. (2007). Nucleoporins Prevent DNA Damage Accumulation by Modulating Ulp1-dependent Sumoylation Processes. Mol. Biol. Cell 18: 2912-2923 [Abstract] [Full Text]  
  • Wang, Z., Jones, G. M., Prelich, G. (2006). Genetic Analysis Connects SLX5 and SLX8 to the SUMO Pathway in Saccharomyces cerevisiae. Genetics 172: 1499-1509 [Abstract] [Full Text]  
  • Motegi, A., Kuntz, K., Majeed, A., Smith, S., Myung, K. (2006). Regulation of Gross Chromosomal Rearrangements by Ubiquitin and SUMO Ligases in Saccharomyces cerevisiae. Mol. Cell. Biol. 26: 1424-1433 [Abstract] [Full Text]