This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Prisco, M.
Right arrow Articles by Baserga, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Prisco, M.
Right arrow Articles by Baserga, R.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, June 2004, p. 5421-5433, Vol. 24, No. 12
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.12.5421-5433.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Role of Pescadillo and Upstream Binding Factor in the Proliferation and Differentiation of Murine Myeloid Cells

Marco Prisco,1 Arianna Maiorana,1 Clara Guerzoni,1 George Calin,1 Bruno Calabretta,1 Renate Voit,2 Ingrid Grummt,2 and Renato Baserga1*

Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107,1 Department of Molecular Biology of the Cell II, German Cancer Research Center, D-69120 Heidelberg, Germany2

Received 6 January 2004/ Returned for modification 4 February 2004/ Accepted 24 March 2004

Pescadillo (PES1) and the upstream binding factor (UBF1) play a role in ribosome biogenesis, which regulates cell size, an important component of cell proliferation. We have investigated the effects of PES1 and UBF1 on the growth and differentiation of cell lines derived from 32D cells, an interleukin-3 (IL-3)-dependent murine myeloid cell line. Parental 32D cells and 32D IGF-IR cells (expressing increased levels of the type 1 insulin-like growth factor I [IGF-I] receptor [IGF-IR]) do not express insulin receptor substrate 1 (IRS-1) or IRS-2. 32D IGF-IR cells differentiate when the cells are shifted from IL-3 to IGF-I. Ectopic expression of IRS-1 inhibits differentiation and transforms 32D IGF-IR cells into a tumor-forming cell line. We found that PES1 and UBF1 increased cell size and/or altered the cell cycle distribution of 32D-derived cells but failed to make them IL-3 independent. PES1 and UBF1 also failed to inhibit the differentiation program initiated by the activation of the IGF-IR, which is blocked by IRS-1. 32D IGF-IR cells expressing PES1 or UBF1 differentiate into granulocytes like their parental cells. In contrast, PES1 and UBF1 can transform mouse embryo fibroblasts that have high levels of endogenous IRS-1 and are not prone to differentiation. Our results provide a model for one of the theories of myeloid leukemia, in which both a stimulus of proliferation and a block of differentiation are required for leukemia development.

* Corresponding author. Mailing address: Kimmel Cancer Center, Thomas Jefferson University, 233 S. 10th St., 624 BLSB, Philadelphia, PA 19107. Phone: (215) 503-4507. Fax: (215) 923-0249. E-mail: B_lupo{at}mail.jci.tju.edu.

Molecular and Cellular Biology, June 2004, p. 5421-5433, Vol. 24, No. 12
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.12.5421-5433.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lerch-Gaggl, A. F., Sun, K., Duncan, S. A. (2007). Light Chain 1 of Microtubule-associated Protein 1B Can Negatively Regulate the Action of Pes1. J. Biol. Chem. 282: 11308-11316 [Abstract] [Full Text]  
  • Sun, H., Tu, X., Baserga, R. (2006). A Mechanism for Cell Size Regulation by the Insulin and Insulin-Like Growth Factor-I Receptors. Cancer Res. 66: 11106-11109 [Abstract] [Full Text]  
  • Sikorski, E. M., Uo, T., Morrison, R. S., Agarwal, A. (2006). Pescadillo Interacts with the Cadmium Response Element of the Human Heme Oxygenase-1 Promoter in Renal Epithelial Cells. J. Biol. Chem. 281: 24423-24430 [Abstract] [Full Text]  
  • Zhao, M., Soderhall, I., Park, J. W., Ma, Y. G., Osaki, T., Ha, N.-C., Wu, C. F., Soderhall, K., Lee, B. L. (2005). A Novel 43-kDa Protein as a Negative Regulatory Component of Phenoloxidase-induced Melanin Synthesis. J. Biol. Chem. 280: 24744-24751 [Abstract] [Full Text]  
  • Wu, A., Tu, X., Prisco, M., Baserga, R. (2005). Regulation of Upstream Binding Factor 1 Activity by Insulin-like Growth Factor I Receptor Signaling. J. Biol. Chem. 280: 2863-2872 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»