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Molecular and Cellular Biology, June 2004, p. 5521-5533, Vol. 24, No. 12
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.12.5521-5533.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Positive and Negative Regulation of Poly(A) Nuclease

David A. Mangus, Matthew C. Evans, Nathan S. Agrin, Mandy Smith, Preetam Gongidi,{dagger}, and Allan Jacobson*

Department of Molecular Genetics and Microbiology University of Massachusetts Medical School, Worcester, Massachusetts 01655

Received 26 September 2003/ Returned for modification 4 November 2003/ Accepted 17 March 2004

PAN, a yeast poly(A) nuclease, plays an important nuclear role in the posttranscriptional maturation of mRNA poly(A) tails. The activity of this enzyme is dependent on its Pan2p and Pan3p subunits, as well as the presence of poly(A)-binding protein (Pab1p). We have identified and characterized the associated network of factors controlling the maturation of mRNA poly(A) tails in yeast and defined its relevant protein-protein interactions. Pan3p, a positive regulator of PAN activity, interacts with Pab1p, thus providing substrate specificity for this nuclease. Pab1p also regulates poly(A) tail trimming by interacting with Pbp1p, a factor that appears to negatively regulate PAN. Pan3p and Pbp1p both interact with themselves and with the C terminus of Pab1p. However, the domains required for Pan3p and Pbp1p binding on Pab1p are distinct. Single amino acid changes that disrupt Pan3p interaction with Pab1p have been identified and define a binding pocket in helices 2 and 3 of Pab1p's carboxy terminus. The importance of these amino acids for Pab1p-Pan3p interaction, and poly(A) tail regulation, is underscored by experiments demonstrating that strains harboring substitutions in these residues accumulate mRNAs with long poly(A) tails in vivo.

* Corresponding author. Mailing address: Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655. Phone: (508) 856-2442. Fax: (508) 856-5920. E-mail: allan.jacobson{at}umassmed.edu.

{dagger} Present address: Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599.

Molecular and Cellular Biology, June 2004, p. 5521-5533, Vol. 24, No. 12
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.12.5521-5533.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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