This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mattern, K. A.
Right arrow Articles by Zijlmans, J. M. J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mattern, K. A.
Right arrow Articles by Zijlmans, J. M. J. M.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, June 2004, p. 5587-5594, Vol. 24, No. 12
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.12.5587-5594.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Dynamics of Protein Binding to Telomeres in Living Cells: Implications for Telomere Structure and Function

Karin A. Mattern,1,{ddagger} Susan J. J. Swiggers,1 Alex L. Nigg,2 Bob Löwenberg,1 Adriaan B. Houtsmuller,2,{dagger} and J. Mark J. M. Zijlmans1,{dagger}*

Department of Hematology,1 Department of Pathology, Josephine Nefkens Institute, Erasmus MC, 3000DR Rotterdam, The Netherlands2

Received 14 October 2003/ Returned for modification 7 December 2003/ Accepted 23 February 2004

Telomeric proteins have an essential role in the regulation of the length of the telomeric DNA tract and in protection against end-to-end chromosome fusion. Telomere organization and how individual proteins are involved in different telomere functions in living cells is largely unknown. By using green fluorescent protein tagging and photobleaching, we investigated in vivo interactions of human telomeric DNA-binding proteins with telomeric DNA. Our results show that telomeric proteins interact with telomeres in a complex dynamic fashion: TRF2, which has a dual role in chromosome end protection and telomere length homeostasis, resides at telomeres in two distinct pools. One fraction (~73%) has binding dynamics similar to TRF1 (residence time of ~44 s). Interestingly, the other fraction of TRF2 binds with similar dynamics as the putative end-protecting factor hPOT1 (residence time of ~11 min). Our data support a dynamic model of telomeres in which chromosome end-protection and telomere length homeostasis are governed by differential binding of telomeric proteins to telomeric DNA.

* Corresponding author. Mailing address: Erasmus MC, Institute of Hematology, P.O. Box 1738, 3000 DR Rotterdam, The Netherlands. Phone: 31-10-4087876. Fax: 31-10-4089470. E-mail: j.zijlmans{at}erasmusmc.nl.

{ddagger} A.B.H. and J.M.J.M.Z. contributed equally to this study.

{dagger} Present address: Department of Pathology, Erasmus MC, Rotterdam.

Molecular and Cellular Biology, June 2004, p. 5587-5594, Vol. 24, No. 12
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.12.5587-5594.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Oganesian, L., Karlseder, J. (2009). Telomeric armor: the layers of end protection. J. Cell Sci. 122: 4013-4025 [Abstract] [Full Text]  
  • Rizzo, A., Salvati, E., Porru, M., D'Angelo, C., Stevens, M. F., D'Incalci, M., Leonetti, C., Gilson, E., Zupi, G., Biroccio, A. (2009). Stabilization of quadruplex DNA perturbs telomere replication leading to the activation of an ATR-dependent ATM signaling pathway. Nucleic Acids Res 37: 5353-5364 [Abstract] [Full Text]  
  • Zhu, Q., Meng, L., Hsu, J. K., Lin, T., Teishima, J., Tsai, R. Y.L. (2009). GNL3L stabilizes the TRF1 complex and promotes mitotic transition. JCB 185: 827-839 [Abstract] [Full Text]  
  • Medhurst, A. L., Warmerdam, D. O., Akerman, I., Verwayen, E. H., Kanaar, R., Smits, V. A. J., Lakin, N. D. (2008). ATR and Rad17 collaborate in modulating Rad9 localisation at sites of DNA damage. J. Cell Sci. 121: 3933-3940 [Abstract] [Full Text]  
  • Aubert, G., Lansdorp, P. M. (2008). Telomeres and Aging. Physiol. Rev. 88: 557-579 [Abstract] [Full Text]  
  • Rossignol, P., Collier, S., Bush, M., Shaw, P., Doonan, J. H. (2007). Arabidopsis POT1A interacts with TERT-V(I8), an N-terminal splicing variant of telomerase. J. Cell Sci. 120: 3678-3687 [Abstract] [Full Text]  
  • O'Connor, M. S., Safari, A., Xin, H., Liu, D., Songyang, Z. (2006). A critical role for TPP1 and TIN2 interaction in high-order telomeric complex assembly. Proc. Natl. Acad. Sci. USA 103: 11874-11879 [Abstract] [Full Text]  
  • Anversa, P., Kajstura, J., Leri, A., Bolli, R. (2006). Life and Death of Cardiac Stem Cells: A Paradigm Shift in Cardiac Biology. Circulation 113: 1451-1463 [Full Text]  
  • Fang, Y., Spector, D. L. (2005). Centromere Positioning and Dynamics in Living Arabidopsis Plants. Mol. Biol. Cell 16: 5710-5718 [Abstract] [Full Text]  
  • Essers, J., Theil, A. F., Baldeyron, C., van Cappellen, W. A., Houtsmuller, A. B., Kanaar, R., Vermeulen, W. (2005). Nuclear Dynamics of PCNA in DNA Replication and Repair. Mol. Cell. Biol. 25: 9350-9359 [Abstract] [Full Text]  
  • Opresko, P. L., Mason, P. A., Podell, E. R., Lei, M., Hickson, I. D., Cech, T. R., Bohr, V. A. (2005). POT1 Stimulates RecQ Helicases WRN and BLM to Unwind Telomeric DNA Substrates. J. Biol. Chem. 280: 32069-32080 [Abstract] [Full Text]  
  • de Lange, T. (2005). Shelterin: the protein complex that shapes and safeguards human telomeres. Genes Dev. 19: 2100-2110 [Abstract] [Full Text]  
  • Yang, Q., Zheng, Y.-L., Harris, C. C. (2005). POT1 and TRF2 Cooperate To Maintain Telomeric Integrity. Mol. Cell. Biol. 25: 1070-1080 [Abstract] [Full Text]  
  • Ye, J. Z.-S., Donigian, J. R., van Overbeek, M., Loayza, D., Luo, Y., Krutchinsky, A. N., Chait, B. T., de Lange, T. (2004). TIN2 Binds TRF1 and TRF2 Simultaneously and Stabilizes the TRF2 Complex on Telomeres. J. Biol. Chem. 279: 47264-47271 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»