This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Berthet, C.
Right arrow Articles by Rouault, J.-P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Berthet, C.
Right arrow Articles by Rouault, J.-P.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, July 2004, p. 5808-5820, Vol. 24, No. 13
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.13.5808-5820.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

CCR4-Associated Factor CAF1 Is an Essential Factor for Spermatogenesis

Cyril Berthet,1,{dagger} Anne-Marie Morera,2 Marie-Jeanne Asensio,2 Marie-Agnes Chauvin,2 Anne-Pierre Morel,1 Frederique Dijoud,3 Jean-Pierre Magaud,1 Philippe Durand,2 and Jean-Pierre Rouault1,2*

UMR INSERM 418-INRA 1245-Université Claude Bernard Lyon I,2 Service d'Anatomopathologie, Hopital Debrousse, F 69322 Lyon Cedex 05,3 INSERM U453, CLB, F 69373 Lyon Cedex 08, France1

Received 8 September 2003/ Returned for modification 16 December 2003/ Accepted 9 April 2004

The CCR4-associated protein CAF1 has been demonstrated to play several roles in the control of transcription and of mRNA decay. To gain further insight into its physiological function, we generated CAF1-deficient mice. They are viable, healthy, and normal in appearance; however, mCAF1–/– male mice are sterile. The crossing of mCAF1+/– mice gave a Mendelian ratio of mCAF1+/+, mCAF1+/–, and mCAF1–/– pups, indicating that haploid mCAF1-deficient germ cells differentiate normally. The onset of the defect occurs during the first wave of spermatogenesis at 19 to 20 days after birth, during progression of pachytene spermatocytes to haploid spermatids and spermatozoa. Early disruption of spermatogenesis was evidenced by Sertoli cell vacuolization and tubular disorganization. The most mature germ cells were the most severely depleted, but progressively all germ cells were affected, giving Sertoli cell-only tubes, large interstitial spaces, and small testes. This phenotype could be linked to a defect(s) in germ cells and/or to inadequate Sertoli cell function, leading to seminiferous tubule disorganization and finally to a total disappearance of germ cells. The mCAF1-deficient mouse provides a new model of failed spermatogenesis in the adult that may be relevant to some cases of human male sterility.

* Corresponding author. Mailing address: UMR INSERM 418- INRA 1245-UCBL, Hop. Debrousse, 29 rue Soeur Bouvier, F 69322 Lyon cedex 05, France. Phone: (33) (0)4.72.38.55.11. Fax: (33) (0)4.78.25.61.68. E-mail: rouault{at}lyon.inserm.fr.

{dagger} Present address: National Cancer Institute, Frederick, MD 21702-1201.

Molecular and Cellular Biology, July 2004, p. 5808-5820, Vol. 24, No. 13
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.13.5808-5820.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Aslam, A., Mittal, S., Koch, F., Andrau, J.-C., Winkler, G. S. (2009). The Ccr4-Not Deadenylase Subunits CNOT7 and CNOT8 Have Overlapping Roles and Modulate Cell Proliferation. Mol. Biol. Cell 20: 3840-3850 [Abstract] [Full Text]  
  • Horiuchi, M., Takeuchi, K., Noda, N., Muroya, N., Suzuki, T., Nakamura, T., Kawamura-Tsuzuku, J., Takahasi, K., Yamamoto, T., Inagaki, F. (2009). Structural Basis for the Antiproliferative Activity of the Tob-hCaf1 Complex. J. Biol. Chem. 284: 13244-13255 [Abstract] [Full Text]  
  • Schwede, A., Ellis, L., Luther, J., Carrington, M., Stoecklin, G., Clayton, C. (2008). A role for Caf1 in mRNA deadenylation and decay in trypanosomes and human cells. Nucleic Acids Res 36: 3374-3388 [Abstract] [Full Text]  
  • Garapaty, S., Mahajan, M. A., Samuels, H. H. (2008). Components of the CCR4-NOT Complex Function as Nuclear Hormone Receptor Coactivators via Association with the NRC-interacting Factor NIF-1. J. Biol. Chem. 283: 6806-6816 [Abstract] [Full Text]  
  • Beilharz, T. H., Preiss, T. (2007). Widespread use of poly(A) tail length control to accentuate expression of the yeast transcriptome. RNA 13: 982-997 [Abstract] [Full Text]  
  • Morita, M., Suzuki, T., Nakamura, T., Yokoyama, K., Miyasaka, T., Yamamoto, T. (2007). Depletion of Mammalian CCR4b Deadenylase Triggers Elevation of the p27Kip1 mRNA Level and Impairs Cell Growth. Mol. Cell. Biol. 27: 4980-4990 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»