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Molecular and Cellular Biology, July 2004, p. 5978-5988, Vol. 24, No. 13
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.13.5978-5988.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Characterization of Mice Lacking the Tetraspanin Superfamily Member CD151

Mark D. Wright,1 Sean M. Geary,2 Stephen Fitter,3,{dagger} Gregory W. Moseley,1 Lai-Man Lau,1 Kuo-Ching Sheng,1 Vasso Apostolopoulos,1 Edouard G. Stanley,3 Denise E. Jackson,1 and Leonie K. Ashman2*

Austin Research Institute,1 Institute of Reproduction and Development, Monash University, Melbourne,3 School of Biomedical Sciences, University of Newcastle, and Hunter Medical Research Institute, Newcastle, and Institute of Medical and Veterinary Science, Adelaide, Australia2

Received 23 December 2003/ Returned for modification 24 January 2004/ Accepted 28 March 2004

The tetraspanin membrane protein CD151 is a broadly expressed molecule noted for its strong molecular associations with integrins, especially {alpha}3ß1, {alpha}6ß1, {alpha}7ß1, and {alpha}6ß4. In vitro functional studies have pointed to a role for CD151 in cell-cell adhesion, cell migration, platelet aggregation, and angiogenesis. It has also been implicated in epithelial tumor progression and metastasis. Here we describe the generation and initial characterization of CD151-null mice. The mice are viable, healthy, and fertile and show normal Mendelian inheritance. They have essentially normal blood and bone marrow cell counts and grossly normal tissue morphology, including hemidesmosomes in skin, and expression of {alpha}3 and {alpha}6 integrins. However, the CD151-null mice do show phenotypes in several different tissue types. An absence of CD151 leads to a minor abnormality in hemostasis, with CD151-null mice showing longer average bleeding times, greater average blood loss, and an increased incidence of rebleeding occurrences. CD151-null keratinocytes migrate poorly in skin explant cultures. Finally, CD151-null T lymphocytes are hyperproliferative in response to in vitro mitogenic stimulation.

* Corresponding author. Mailing address: School of Biomedical Sciences, University of Newcastle, Callaghan, NSW 2308, Australia. Phone: 61 2 4921 7947. Fax: 61 2 4921 6903. E-mail: leonie.ashman{at}newcastle.edu.au.

{dagger} Present address: Department of Gastroenterology, Flinders University, Adelaide, Australia.

Molecular and Cellular Biology, July 2004, p. 5978-5988, Vol. 24, No. 13
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.13.5978-5988.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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