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Molecular and Cellular Biology, August 2004, p. 6907-6918, Vol. 24, No. 16
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.16.6907-6918.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Kinetochores Prevent Repair of UV Damage in Saccharomyces cerevisiae Centromeres

Christoph Capiaghi, The Vinh Ho, and Fritz Thoma*

Institut für Zellbiologie, ETH-Hönggerberg, CH-8093 Zürich, Switzerland

Received 8 April 2004/ Returned for modification 9 May 2004/ Accepted 18 May 2004

Centromeres form specialized chromatin structures termed kinetochores which are required for accurate segregation of chromosomes. DNA lesions might disrupt protein-DNA interactions, thereby compromising segregation and genome stability. We show that yeast centromeres are heavily resistant to removal of UV-induced DNA lesions by two different repair systems, photolyase and nucleotide excision repair. Repair resistance persists in G1- and G2/M-arrested cells. Efficient repair was obtained only by disruption of the kinetochore structure in a ndc10-1 mutant, but not in cse4-1 and cbf1{Delta} mutants. Moreover, UV photofootprinting and DNA repair footprinting showed that centromere proteins cover about 120 bp of the centromere elements CDEII and CDEIII, including 20 bp of flanking CDEIII. Thus, DNA lesions do not appear to disrupt protein-DNA interactions in the centromere. Maintaining a stable kinetochore structure seems to be more important for the cell than immediate removal of DNA lesions. It is conceivable that centromeres are repaired by postreplication repair pathways.

* Corresponding author. Mailing address: Institut für Zellbiologie, ETH-Hönggerberg, CH-8093 Zürich, Switzerland. Phone: 41 1 633 33 23. Fax: 41 1 633 10 69. E-mail: thoma{at}cell.biol.ethz.ch.

Molecular and Cellular Biology, August 2004, p. 6907-6918, Vol. 24, No. 16
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.16.6907-6918.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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