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Molecular and Cellular Biology, August 2004, p. 7151-7162, Vol. 24, No. 16
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.16.7151-7162.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Glut4 Storage Vesicles without Glut4: Transcriptional Regulation of Insulin-Dependent Vesicular Traffic

Danielle N. Gross, Stephen R. Farmer, and Paul F. Pilch*

Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118

Received 6 January 2004/ Returned for modification 23 February 2004/ Accepted 20 May 2004

Two families of transcription factors that play a major role in the development of adipocytes are the CCAAT/enhancer-binding proteins (C/EBPs) and the peroxisome proliferator-activated receptors (PPARs), in particular PPAR{gamma}. Ectopic expression of either C/EBP{alpha} or PPAR{gamma} in NIH 3T3 fibroblasts results in the conversion of these cells to adipocyte-like cells replete with fat droplets. NIH 3T3 cells ectopically expressing C/EBP{alpha} (NIH-C/EBP{alpha}) differentiate into adipocytes and exhibit insulin-stimulated glucose uptake, whereas NIH 3T3 cells ectopically expressing PPAR{gamma} (NIH-PPAR{gamma}) differentiate but do not exhibit any insulin-stimulated glucose uptake, nor do they express any C/EBP{alpha}. The reason for the lack of insulin-responsive glucose uptake in the NIH-PPAR{gamma} cells is their virtual lack of the insulin-responsive glucose transporter, Glut4. The NIH-PPAR{gamma} cells express functionally active components of the insulin receptor-signaling pathway (the insulin receptor, IRS-1, phosphatidylinositol 3-kinase, and Akt2) at levels comparable to those in responsive cell lines. They also express components of the insulin-sensitive vesicular transport machinery, namely, VAMP2, syntaxin-4, and IRAP, the last of these being the other marker of insulin-regulated vesicular traffic along with Glut4. Interestingly, the NIH-PPAR{gamma} cells show normal insulin-dependent translocation of IRAP and form an insulin-responsive vesicular compartment as assessed by cell surface biotinylation and sucrose velocity gradient analysis, respectively. Moreover, expression of a Glut4-myc construct in the NIH-PPAR{gamma} cells results in its insulin-dependent translocation to the plasma membrane as assessed by immunofluorescence and Western blot analysis. Based on these data, we conclude that major role of C/EBP{alpha} in the context of the NIH-PPAR{gamma} cells is to regulate Glut4 expression. The differentiated cells possess a large insulin-sensitive vesicular compartment with negligible Glut4, and Glut4 translocation can be reconstituted on expression of this transporter.

* Corresponding author. Mailing address: Department of Biochemistry, Boston University School of Medicine, 715 Albany St., Boston, MA 02118. Phone: (617) 638-4044. Fax: (617) 638-4208. E-mail: ppilch{at}bu.edu.

Molecular and Cellular Biology, August 2004, p. 7151-7162, Vol. 24, No. 16
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.16.7151-7162.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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