This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Garnier, O.
Right arrow Articles by Meyer, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Garnier, O.
Right arrow Articles by Meyer, E.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, September 2004, p. 7370-7379, Vol. 24, No. 17
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.17.7370-7379.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

RNA-Mediated Programming of Developmental Genome Rearrangements in Paramecium tetraurelia

Olivier Garnier,{dagger} Vincent Serrano,{dagger} Sandra Duharcourt, and Eric Meyer*

Laboratoire de Génétique Moléculaire, CNRS UMR 8541, Ecole Normale Supérieure, Paris, France

Received 13 February 2004/ Returned for modification 24 March 2004/ Accepted 5 June 2004

The germ line genome of ciliates is extensively rearranged during development of the somatic macronucleus. Numerous sequences are eliminated, while others are amplified to a high ploidy level. In the Paramecium aurelia group of species, transformation of the maternal macronucleus with transgenes at high copy numbers can induce the deletion of homologous genes in sexual progeny, when a new macronucleus develops from the wild-type germ line. We show that this trans-nuclear effect correlates with homology-dependent silencing of maternal genes before autogamy and with the accumulation of ~22- to 23-nucleotide (nt) RNA molecules. The same effects are induced by feeding cells before meiosis with bacteria containing double-stranded RNA, suggesting that small interfering RNA-like molecules can target deletions. Furthermore, experimentally induced macronuclear deletions are spontaneously reproduced in subsequent sexual generations, and reintroduction of the missing gene into the variant macronucleus restores developmental amplification in sexual progeny. We discuss the possible roles of the ~22- to 23-nt RNAs in the targeting of deletions and the implications for the RNA-mediated genome-scanning process that is thought to determine developmentally regulated rearrangements in ciliates.

* Corresponding author. Mailing address: Laboratoire de Génétique Moléculaire, CNRS UMR 8541, Ecole Normale Supérieure, 46, rue d'Ulm, 75005 Paris, France. Phone: 33 1 44 32 39 48. Fax: 33 1 44 32 39 41. E-mail: emeyer{at}wotan.ens.fr.

{dagger} O.G. and V.S. contributed equally to this work.

Molecular and Cellular Biology, September 2004, p. 7370-7379, Vol. 24, No. 17
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.17.7370-7379.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Baudry, C., Malinsky, S., Restituito, M., Kapusta, A., Rosa, S., Meyer, E., Betermier, M. (2009). PiggyMac, a domesticated piggyBac transposase involved in programmed genome rearrangements in the ciliate Paramecium tetraurelia. Genes Dev. 23: 2478-2483 [Abstract] [Full Text]  
  • Lepere, G., Nowacki, M., Serrano, V., Gout, J.-F., Guglielmi, G., Duharcourt, S., Meyer, E. (2009). Silencing-associated and meiosis-specific small RNA pathways in Paramecium tetraurelia. Nucleic Acids Res 37: 903-915 [Abstract] [Full Text]  
  • Gratias, A., Lepere, G., Garnier, O., Rosa, S., Duharcourt, S., Malinsky, S., Meyer, E., Betermier, M. (2008). Developmentally programmed DNA splicing in Paramecium reveals short-distance crosstalk between DNA cleavage sites. Nucleic Acids Res 36: 3244-3251 [Abstract] [Full Text]  
  • Lepere, G., Betermier, M., Meyer, E., Duharcourt, S. (2008). Maternal noncoding transcripts antagonize the targeting of DNA elimination by scanRNAs in Paramecium tetraurelia. Genes Dev. 22: 1501-1512 [Abstract] [Full Text]  
  • Gogendeau, D., Beisson, J., de Loubresse, N. G., Le Caer, J.-P., Ruiz, F., Cohen, J., Sperling, L., Koll, F., Klotz, C. (2007). An Sfi1p-Like Centrin-Binding Protein Mediates Centrin-Based Ca2+-Dependent Contractility in Paramecium tetraurelia. Eukaryot Cell 6: 1992-2000 [Abstract] [Full Text]  
  • Robinson, T., Katz, L. A. (2007). Non-Mendelian Inheritance of Paralogs of 2 Cytoskeletal Genes in the Ciliate Chilodonella uncinata. Mol Biol Evol 24: 2495-2503 [Abstract] [Full Text]  
  • Liu, Y., Taverna, S. D., Muratore, T. L., Shabanowitz, J., Hunt, D. F., Allis, C. D. (2007). RNAi-dependent H3K27 methylation is required for heterochromatin formation and DNA elimination in Tetrahymena. Genes Dev. 21: 1530-1545 [Abstract] [Full Text]  
  • Howard-Till, R. A., Yao, M.-C. (2006). Induction of Gene Silencing by Hairpin RNA Expression in Tetrahymena thermophila Reveals a Second Small RNA Pathway. Mol. Cell. Biol. 26: 8731-8742 [Abstract] [Full Text]  
  • Matsuda, A., Forney, J. D. (2006). The SUMO Pathway Is Developmentally Regulated and Required for Programmed DNA Elimination in Paramecium tetraurelia. Eukaryot Cell 5: 806-815 [Abstract] [Full Text]  
  • Preer, J. R. Jr. (2006). Sonneborn and the cytoplasm.. Genetics 172: 1373-1377 [Full Text]  
  • Juranek, S. A., Rupprecht, S., Postberg, J., Lipps, H. J. (2005). snRNA and Heterochromatin Formation Are Involved in DNA Excision during Macronuclear Development in Stichotrichous Ciliates. Eukaryot Cell 4: 1934-1941 [Abstract] [Full Text]  
  • Chang, W.-J., Bryson, P. D., Liang, H., Shin, M. K., Landweber, L. F. (2005). The evolutionary origin of a complex scrambled gene. Proc. Natl. Acad. Sci. USA 102: 15149-15154 [Abstract] [Full Text]  
  • Malone, C. D., Anderson, A. M., Motl, J. A., Rexer, C. H., Chalker, D. L. (2005). Germ Line Transcripts Are Processed by a Dicer-Like Protein That Is Essential for Developmentally Programmed Genome Rearrangements of Tetrahymena thermophila. Mol. Cell. Biol. 25: 9151-9164 [Abstract] [Full Text]  
  • Matsuda, A., Forney, J. D. (2005). Analysis of Paramecium tetraurelia A-51 Surface Antigen Gene Mutants Reveals Positive-Feedback Mechanisms for Maintenance of Expression and Temperature-Induced Activation. Eukaryot Cell 4: 1613-1619 [Abstract] [Full Text]  
  • Bernstein, E., Allis, C. D. (2005). RNA meets chromatin. Genes Dev. 19: 1635-1655 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»