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Molecular and Cellular Biology, September 2004, p. 7483-7490, Vol. 24, No. 17
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.17.7483-7490.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SMG-1 Is a Phosphatidylinositol Kinase-Related Protein Kinase Required for Nonsense-Mediated mRNA Decay in Caenorhabditis elegans

Andrew Grimson,{dagger} Sean O'Connor,{dagger},{ddagger} Carrie Loushin Newman, and Philip Anderson*

Department of Genetics, University of Wisconsin, Madison, Wisconsin

Received 1 March 2004/ Returned for modification 9 April 2004/ Accepted 1 June 2004

Eukaryotic messenger RNAs containing premature stop codons are selectively and rapidly degraded, a phenomenon termed nonsense-mediated mRNA decay (NMD). Previous studies with both Caenohabditis elegans and mammalian cells indicate that SMG-2/human UPF1, a central regulator of NMD, is phosphorylated in an SMG-1-dependent manner. We report here that smg-1, which is required for NMD in C. elegans, encodes a protein kinase of the phosphatidylinositol kinase superfamily of protein kinases. We identify null alleles of smg-1 and demonstrate that SMG-1 kinase activity is required in vivo for NMD and in vitro for SMG-2 phosphorylation. SMG-1 and SMG-2 coimmunoprecipitate from crude extracts, and this interaction is maintained in smg-3 and smg-4 mutants, both of which are required for SMG-2 phosphorylation in vivo and in vitro. SMG-2 is located diffusely through the cytoplasm, and its location is unaltered in mutants that disrupt the cycle of SMG-2 phosphorylation. We discuss the role of SMG-2 phosphorylation in NMD.

* Corresponding author. Mailing address: Department of Genetics, University of Wisconsin, 445 Henry Mall, Madison, WI 53706. Phone: (608) 263-8429. Fax: (608) 262-2976. E-mail: andersn{at}wisc.edu.

{dagger} A.G. and S.O. contributed equally to this work.

{ddagger} Present address: Department of Blood and Marrow Transplantation, University of Texas, Houston, TX 77030.

Molecular and Cellular Biology, September 2004, p. 7483-7490, Vol. 24, No. 17
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.17.7483-7490.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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