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Molecular and Cellular Biology, September 2004, p. 8227-8235, Vol. 24, No. 18
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.18.8227-8235.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

The Swi/Snf Chromatin Remodeling Complex Is Required for Ribosomal DNA and Telomeric Silencing in Saccharomyces cerevisiae

Vardit Dror1,2 and Fred Winston1*

Department of Genetics, Harvard Medical School, Boston, Massachusetts,1 Department of Biochemistry, Tel Aviv University, Tel Aviv, Israel2

Received 14 May 2004/ Returned for modification 17 June 2004/ Accepted 25 June 2004

The Swi/Snf chromatin remodeling complex has been previously demonstrated to be required for transcriptional activation and repression of a subset of genes in Saccharomyces cerevisiae. In this work we demonstrate that Swi/Snf is also required for repression of RNA polymerase II-dependent transcription in the ribosomal DNA (rDNA) locus (rDNA silencing). This repression appears to be independent of both Sir2 and Set1, two factors known to be required for rDNA silencing. In contrast to many other rDNA silencing mutants that have elevated levels of rDNA recombination, snf2{Delta} mutants have a significantly decreased level of rDNA recombination. Additional studies have demonstrated that Swi/Snf is also required for silencing of genes near telomeres while having no detectable effect on silencing of HML or HMR.

* Corresponding author. Mailing address: Department of Genetics, Harvard Medical School, 77 Ave. Louis Pasteur, Boston, MA 02115. Phone: (617) 432-7768. Fax: (617) 432-6506. E-mail: winston{at}genetics.med.harvard.edu.

Molecular and Cellular Biology, September 2004, p. 8227-8235, Vol. 24, No. 18
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.18.8227-8235.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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