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Molecular and Cellular Biology, January 2004, p. 537-549, Vol. 24, No. 2
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.2.537-549.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

BASP1 Is a Transcriptional Cosuppressor for the Wilms' Tumor Suppressor Protein WT1

Brian Carpenter,1,{dagger} Kathryn J. Hill,1,{dagger} Marika Charalambous,2 Kate J. Wagner,1 Diya Lahiri,2 Dominic I. James,1 Jens S. Andersen,3 Valérie Schumacher,4 Brigitte Royer-Pokora,4 Matthias Mann,3 Andrew Ward,2 and Stefan G. E. Roberts1*

School of Biological Sciences, University of Manchester, Manchester M13 9PT,1 Developmental Biology Program and Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, United Kingdom,2 Protein Interaction Laboratory, University of Southern Denmark-Odense, DK-5230 Odense, Denmark,3 Institute of Human Genetics and Anthropology, University of Duesseldorf, D40001 Düsseldorf, Germany4

Received 25 July 2003/ Returned for modification 18 September 2003/ Accepted 20 October 2003

The Wilms' tumor suppressor protein WT1 is a transcriptional regulator that plays a key role in the development of the kidneys. The transcriptional activation domain of WT1 is subject to regulation by a suppression region within the N terminus of WT1. Using a functional assay, we provide direct evidence that this requires a transcriptional cosuppressor, which we identify as brain acid soluble protein 1 (BASP1). WT1 and BASP1 associate within the nuclei of cells that naturally express both proteins. BASP1 can confer WT1 cosuppressor activity in transfection assays, and elimination of endogenous BASP1 expression augments transcriptional activation by WT1. BASP1 is present in the developing nephron structures of the embryonic kidney and, coincident with that of WT1, its expression is restricted to the highly specialized podocyte cells of the adult kidney. Taken together, our results show that BASP1 is a WT1-associated factor that can regulate WT1 transcriptional activity.

* Corresponding author. Mailing address: School of Biological Sciences, G.186 Stopford Building, University of Manchester, Oxford Rd., Manchester M13 9PT, United Kingdom. Phone: 0161 275 5758. Fax: 0161 275 5600. E-mail: stefan.roberts{at}man.ac.uk.

{dagger} B.C. and K.J.H contributed equally to this paper.

Molecular and Cellular Biology, January 2004, p. 537-549, Vol. 24, No. 2
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.2.537-549.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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