This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Duina, A. A. Articles by Winston, F. Search for Related Content PubMed PubMed Citation Articles by Duina, A. A. Articles by Winston, F.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, January 2004, p. 561-572, Vol. 24, No. 2
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.2.561-572.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Analysis of a Mutant Histone H3 That Perturbs the Association of Swi/Snf with Chromatin

Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115

Received 23 July 2003/ Returned for modification 23 September 2002/ Accepted 14 October 2003

We have isolated new histone H3 mutants in Saccharomyces cerevisiae that confer phenotypes indicative of transcriptional defects. Here we describe the characterization of one such mutant, encoded by the hht2-11 allele, which contains the single amino acid change L61W in the globular domain of H3. Whole-genome expression analyses show that the hht2-11 mutation confers pleiotropic transcriptional defects and that many of the genes it affects are normally controlled by the Swi/Snf chromatin remodeling complex. Furthermore, we show that Swi/Snf occupancy at two promoters, PHO84 and SER3, is reduced in hht2-11 mutants. Detailed studies of the PHO84 promoter suggest that the hht2-11 mutation impairs Swi/Snf association with chromatin in a direct fashion. Taken together, our results strongly suggest that the integrity of the globular domain of histone H3 is an important determinant in the ability of Swi/Snf to associate with chromatin.

This article has been cited by other articles:

• He, Q., Yu, C., Morse, R. H. (2008). Dispersed Mutations in Histone H3 That Affect Transcriptional Repression and Chromatin Structure of the CHA1 Promoter in Saccharomyces cerevisiae. Eukaryot Cell 7: 1649-1660 [Abstract] [Full Text]  
• Reynolds, T. B., Jansen, A., Peng, X., Fink, G. R. (2008). Mat Formation in Saccharomyces cerevisiae Requires Nutrient and pH Gradients. Eukaryot Cell 7: 122-130 [Abstract] [Full Text]  
• Duina, A. A., Rufiange, A., Bracey, J., Hall, J., Nourani, A., Winston, F. (2007). Evidence that the Localization of the Elongation Factor Spt16 Across Transcribed Genes Is Dependent Upon Histone H3 Integrity in Saccharomyces cerevisiae. Genetics 177: 101-112 [Abstract] [Full Text]  
• Matsubara, K., Sano, N., Umehara, T., Horikoshi, M. (2007). Global analysis of functional surfaces of core histones with comprehensive point mutants.. GENES CELLS 12: 13-33 [Abstract] [Full Text]  
• Fry, C. J., Norris, A., Cosgrove, M., Boeke, J. D., Peterson, C. L. (2006). The LRS and SIN Domains: Two Structurally Equivalent but Functionally Distinct Nucleosomal Surfaces Required for Transcriptional Silencing. Mol. Cell. Biol. 26: 9045-9059 [Abstract] [Full Text]  
• Martin, D. G. E., Baetz, K., Shi, X., Walter, K. L., MacDonald, V. E., Wlodarski, M. J., Gozani, O., Hieter, P., Howe, L. (2006). The Yng1p Plant Homeodomain Finger Is a Methyl-Histone Binding Module That Recognizes Lysine 4-Methylated Histone H3. Mol. Cell. Biol. 26: 7871-7879 [Abstract] [Full Text]  
• Martin, D. G. E., Grimes, D. E., Baetz, K., Howe, L. (2006). Methylation of Histone H3 Mediates the Association of the NuA3 Histone Acetyltransferase with Chromatin. Mol. Cell. Biol. 26: 3018-3028 [Abstract] [Full Text]  
• Hyland, E. M., Cosgrove, M. S., Molina, H., Wang, D., Pandey, A., Cottee, R. J., Boeke, J. D. (2005). Insights into the Role of Histone H3 and Histone H4 Core Modifiable Residues in Saccharomyces cerevisiae. Mol. Cell. Biol. 25: 10060-10070 [Abstract] [Full Text]  
• Martens, J. A., Wu, P.-Y. J., Winston, F. (2005). Regulation of an intergenic transcript controls adjacent gene transcription in Saccharomyces cerevisiae. Genes Dev. 19: 2695-2704 [Abstract] [Full Text]  
• Ozdemir, A., Spicuglia, S., Lasonder, E., Vermeulen, M., Campsteijn, C., Stunnenberg, H. G., Logie, C. (2005). Characterization of Lysine 56 of Histone H3 as an Acetylation Site in Saccharomyces cerevisiae. J. Biol. Chem. 280: 25949-25952 [Abstract] [Full Text]