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Molecular and Cellular Biology, January 2004, p. 561-572, Vol. 24, No. 2
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.2.561-572.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Analysis of a Mutant Histone H3 That Perturbs the Association of Swi/Snf with Chromatin

Andrea A. Duina and Fred Winston*

Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115

Received 23 July 2003/ Returned for modification 23 September 2002/ Accepted 14 October 2003

We have isolated new histone H3 mutants in Saccharomyces cerevisiae that confer phenotypes indicative of transcriptional defects. Here we describe the characterization of one such mutant, encoded by the hht2-11 allele, which contains the single amino acid change L61W in the globular domain of H3. Whole-genome expression analyses show that the hht2-11 mutation confers pleiotropic transcriptional defects and that many of the genes it affects are normally controlled by the Swi/Snf chromatin remodeling complex. Furthermore, we show that Swi/Snf occupancy at two promoters, PHO84 and SER3, is reduced in hht2-11 mutants. Detailed studies of the PHO84 promoter suggest that the hht2-11 mutation impairs Swi/Snf association with chromatin in a direct fashion. Taken together, our results strongly suggest that the integrity of the globular domain of histone H3 is an important determinant in the ability of Swi/Snf to associate with chromatin.


* Corresponding author. Mailing address: Department of Genetics, Harvard Medical School, 77 Ave. Louis Pasteur, Boston, MA 02115. Phone: (617) 432-7768. Fax: (617) 432-6506. E-mail: winston{at}genetics.med.harvard.edu.


Molecular and Cellular Biology, January 2004, p. 561-572, Vol. 24, No. 2
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.2.561-572.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.




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